Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HF909_RS15925 Genome accession   NZ_CP051169
Coordinates   3297300..3298799 (+) Length   499 a.a.
NCBI ID   WP_193026583.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN2474     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3292300..3303799
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF909_RS15900 (HF909_16000) - 3293499..3294482 (+) 984 WP_193026578.1 KpsF/GutQ family sugar-phosphate isomerase -
  HF909_RS15905 (HF909_16005) - 3294514..3295101 (+) 588 WP_193026579.1 KdsC family phosphatase -
  HF909_RS15910 (HF909_16010) lptC 3295103..3295735 (+) 633 WP_193026580.1 LPS export ABC transporter periplasmic protein LptC -
  HF909_RS15915 (HF909_16015) lptA 3295805..3296380 (+) 576 WP_193026581.1 lipopolysaccharide transport periplasmic protein LptA -
  HF909_RS15920 (HF909_16020) lptB 3296414..3297202 (+) 789 WP_193026582.1 LPS export ABC transporter ATP-binding protein -
  HF909_RS15925 (HF909_16025) rpoN/rpoN1 3297300..3298799 (+) 1500 WP_193026583.1 RNA polymerase factor sigma-54 Machinery gene
  HF909_RS15930 (HF909_16030) hpf 3298851..3299204 (+) 354 WP_193026584.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  HF909_RS15935 (HF909_16035) ptsN 3299553..3300008 (+) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  HF909_RS15940 (HF909_16040) hprK 3300079..3301053 (+) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  HF909_RS15945 (HF909_16045) - 3301242..3301577 (+) 336 WP_193028556.1 PsiF family protein -
  HF909_RS15950 (HF909_16050) rapZ 3301671..3302561 (+) 891 WP_193026585.1 RNase adapter RapZ -
  HF909_RS15955 (HF909_16055) - 3302639..3303289 (+) 651 WP_193026586.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55184.55 Da        Isoelectric Point: 4.8441

>NTDB_id=436547 HF909_RS15925 WP_193026583.1 3297300..3298799(+) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain RUN2474]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVSALQSSSATPMPTE
SPPPTANGADHPDTAEASYENGSDFDSDYGTDRSDWSLDDFARKPQSDEDDRAPMQLRDAEPTQREFLMEQLAPLKLSPR
DKGLVIFLIESLDDEGYLTGSLDEILADLPPELEVELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
AIVNQHLELLAARDYTRLKKALSVDDGALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=436547 HF909_RS15925 WP_193026583.1 3297300..3298799(+) (rpoN/rpoN1) [Ralstonia pseudosolanacearum strain RUN2474]
ATGAAACAGTCGCTCCAGCTCCGCCTTTCCCAGCATCTGGCGCTCACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAACTGCAACAGGAAGTCGAGCAGGCGCTGACTGAGAACCCGCTGCTTGAGCGCGAGAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCCGACGGCTCGGTCAGCGCCCTGCAGAGCAGCAGTGCCACCCCGATGCCGACCGAA
TCGCCGCCGCCCACCGCCAACGGCGCGGACCATCCCGACACGGCCGAGGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTACGGCACCGATCGGTCCGACTGGAGCCTCGACGACTTCGCGCGCAAACCCCAGTCCGACGAAGACGACCGCGCGC
CGATGCAGCTGCGCGATGCCGAACCCACGCAGCGCGAATTCCTGATGGAACAGCTCGCGCCGCTCAAGCTGTCCCCGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGATCTGCCGCCCGAACTCGAGGTCGAGCTCGATGAATTGCAAGCCGCGCTGCGGATGCTGCAGAGCTTCGATCCGGCCG
GCATCGGTGCGCGCTCGGCGGCCGAATGCCTGACGCTGCAGCTGCAGCGCCTGGACTCCCCAGCCAAGCCGCTGGCGCTG
GCCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGGCTGAAGAAAGCGCTGTCCGTCGATGATGG
CGCGCTGAAGGCCGCGCATGAGCTGATCCGCTCGCTGGCGCCCTTCCCGGGCCACGCCTTCGGGCGGGCCGAGGCCGACT
TCGTCGTGCCCGACGTGGTGGTGCGCAAGACCGCCGCGGGCTGGATGGCACAGCTGAACCCCGACGTGATGCCACGCCTG
CGCATCAACGACATGTATGCGCAGATCCTGCGCAGCAGCCGTGGCGAATCGGGCGCCGCCAACCTGCAGCAGAAGCTGCA
AGAAGCGCGCTGGCTGATCAAAAATATCCAGCAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCCATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAATACATGGCAACCCCCATGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACCGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAGATCCCAGCAGTGAATCTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

98.196

100

0.982