Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HEQ85_RS06295 Genome accession   NZ_CP051168
Coordinates   1424689..1426716 (-) Length   675 a.a.
NCBI ID   WP_199248772.1    Uniprot ID   -
Organism   [Phormidium] sp. ETS-05     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1419689..1431716
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HEQ85_RS06275 - 1420121..1421236 (-) 1116 WP_199248768.1 tol-pal system YbgF family protein -
  HEQ85_RS06280 - 1421391..1421993 (-) 603 WP_199248769.1 hypothetical protein -
  HEQ85_RS06285 - 1422119..1423240 (-) 1122 WP_199248770.1 type II secretion system F family protein -
  HEQ85_RS06290 - 1423374..1424483 (-) 1110 WP_199248771.1 type IV pilus twitching motility protein PilT -
  HEQ85_RS06295 pilB/pilB1 1424689..1426716 (-) 2028 WP_199248772.1 GspE/PulE family protein Machinery gene
  HEQ85_RS06300 - 1426736..1426918 (-) 183 WP_199248773.1 hypothetical protein -
  HEQ85_RS28745 - 1427059..1427190 (+) 132 WP_255552827.1 hypothetical protein -
  HEQ85_RS06305 grpE 1427388..1428155 (+) 768 WP_233258588.1 nucleotide exchange factor GrpE -
  HEQ85_RS06310 dnaK 1428294..1430201 (+) 1908 WP_199248774.1 molecular chaperone DnaK -
  HEQ85_RS06315 dnaJ 1430285..1431409 (+) 1125 WP_199248775.1 molecular chaperone DnaJ -
  HEQ85_RS06320 - 1431409..1431666 (+) 258 WP_199248776.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 675 a.a.        Molecular weight: 74929.83 Da        Isoelectric Point: 5.4682

>NTDB_id=436516 HEQ85_RS06295 WP_199248772.1 1424689..1426716(-) (pilB/pilB1) [[Phormidium] sp. ETS-05]
MTNSSISPKQALVTTSGKFSPFENKLIKAGYANPIQIGEANQQSRKEGKPFTEALESLVQKPIPPELLREYKLHQLFEIK
ILYGVEAFDPEIREVSLETLSELIDSVISIEMCRLHRLLPLEKSVGEPPSVLVGMVDPENITALDDLKRILQPKGWGLRR
RALAPEDYRKLLDQYLEGKAEEEAQRKKKEEEKDKQVDLTVDIDAFANVDIGEAEAEGNDDMDINKSLQGAESAPIVKTV
NQILAKALTDAVSDIHVEPQEEFLRVRMRKDGVLQEIYKLPKKIVNPLVARFKIIADLDIAERRNAQDGRIRRVFQGRTV
DFRVNVVPSRFGEKICMRILDNSSTQLGLDSLITDQEALAKVREFASRPFGLILVTGPTGSGKSTSLYSVLAERNEVGVN
ISTAEDPIEYALPGITQCQVIRAKGLDFTALLRAFMRQDPDIILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAI
ARLDEMGVEPFMVAGSLIGISAQRLMRRVCGTCRQAYSPTPDELARYGLSASRESGATFYKANFIAPNDRKAADEAGSLC
PKCRGLGYKGRVGVYEVLKNSDQISALITQRASTAMIKERAEEEGMVSLLPYSINLVREGHTTFEEVDRVTFTDTGLDAE
LKRRRKIGLTCKACGAGLQQEWMDCPYCLTPRFQE

Nucleotide


Download         Length: 2028 bp        

>NTDB_id=436516 HEQ85_RS06295 WP_199248772.1 1424689..1426716(-) (pilB/pilB1) [[Phormidium] sp. ETS-05]
ATGACCAACTCGTCCATCTCACCAAAACAAGCTCTCGTTACCACCAGTGGCAAGTTTTCTCCTTTTGAAAATAAACTGAT
TAAAGCTGGTTATGCCAACCCTATACAAATAGGAGAAGCGAATCAGCAAAGCCGGAAGGAGGGCAAGCCATTTACTGAGG
CGCTCGAAAGTCTGGTGCAAAAGCCGATCCCTCCAGAATTGCTGCGGGAATACAAGCTGCATCAGTTGTTTGAAATCAAA
ATCCTCTACGGCGTCGAGGCTTTCGACCCAGAGATAAGAGAAGTTTCTCTGGAGACGCTATCGGAACTGATAGACTCGGT
TATCAGTATCGAAATGTGTCGTCTGCATCGTCTGCTACCTCTGGAAAAGAGTGTGGGGGAGCCGCCTTCGGTGTTGGTGG
GGATGGTAGATCCGGAAAATATCACGGCTTTGGATGATTTAAAGCGGATTTTACAACCCAAGGGATGGGGGTTGCGGCGG
CGAGCCCTAGCGCCGGAGGACTATCGCAAGCTGCTAGACCAATACCTGGAAGGTAAAGCCGAAGAGGAAGCACAGCGCAA
GAAGAAAGAAGAAGAGAAAGACAAGCAGGTGGATTTGACTGTCGATATCGACGCTTTCGCTAATGTGGATATTGGGGAGG
CGGAAGCTGAGGGCAATGACGATATGGACATCAATAAGTCCCTACAAGGGGCTGAATCGGCGCCGATCGTCAAAACGGTC
AACCAAATCCTGGCCAAAGCATTGACGGACGCGGTATCGGACATCCACGTGGAACCCCAAGAAGAGTTTCTCCGGGTGCG
GATGCGGAAAGATGGGGTACTCCAAGAAATCTACAAGTTGCCGAAAAAGATTGTTAATCCTTTGGTGGCGCGCTTCAAAA
TTATCGCAGATTTGGACATCGCCGAACGCCGGAATGCCCAAGATGGTCGCATCCGCCGGGTGTTTCAGGGGCGCACGGTG
GATTTTCGGGTGAATGTGGTTCCTAGCCGCTTCGGTGAGAAAATCTGTATGCGGATTTTGGATAACTCTTCGACGCAGCT
AGGTTTGGACTCGTTGATTACTGACCAGGAGGCGCTGGCGAAGGTGCGGGAGTTCGCCAGCCGTCCGTTTGGCCTGATTT
TGGTGACGGGACCGACGGGTTCGGGTAAGTCAACATCTTTGTATTCGGTGTTGGCGGAACGCAATGAGGTGGGGGTAAAT
ATCAGTACGGCGGAAGACCCCATCGAATATGCTCTACCGGGGATTACCCAGTGTCAGGTGATTCGGGCGAAGGGTCTGGA
TTTTACGGCCCTGCTGCGGGCGTTTATGCGTCAAGACCCGGATATTATTCTGGTGGGAGAAACGCGGGATAAGGAAACGG
CGAAAACGGCGATCGAGGCGGCGCTCACGGGTCACTTGGTGCTGACTACTCTGCACACTAACGACGCGGCGGGGGCGATC
GCTCGTCTGGATGAAATGGGCGTGGAGCCGTTCATGGTCGCCGGTTCTCTCATCGGTATTTCTGCCCAACGTTTAATGCG
CCGAGTTTGTGGCACCTGTCGCCAAGCCTATAGCCCCACGCCGGACGAACTGGCCCGCTATGGTCTCTCTGCGTCTCGGG
AATCAGGAGCCACGTTCTATAAAGCTAATTTCATCGCTCCGAACGATCGTAAAGCCGCAGATGAAGCTGGTTCCCTCTGT
CCCAAGTGTCGCGGCTTAGGCTACAAAGGTCGCGTGGGCGTGTATGAAGTCCTGAAAAACAGTGATCAAATCTCCGCCCT
CATTACCCAACGCGCTTCAACGGCCATGATTAAAGAACGCGCCGAGGAAGAGGGCATGGTGTCTCTGCTCCCGTACAGCA
TCAACCTGGTGCGGGAAGGTCACACTACCTTTGAAGAGGTCGATCGTGTCACCTTCACTGACACCGGTTTAGACGCGGAA
CTGAAGCGGCGGCGCAAAATTGGCCTCACCTGTAAGGCTTGTGGCGCCGGACTGCAGCAGGAATGGATGGACTGCCCTTA
CTGCTTGACCCCGCGTTTCCAAGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(75-180)

Type II/IV secretion system protein (T2SSE)

(242-508)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

59.911

100

0.6