Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HCG48_RS24695 Genome accession   NZ_CP051167
Coordinates   6174850..6176862 (-) Length   670 a.a.
NCBI ID   WP_168571551.1    Uniprot ID   -
Organism   Oxynema aestuarii AP17     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6169850..6181862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HCG48_RS24660 (HCG48_24660) - 6170214..6170423 (+) 210 WP_168571545.1 DUF2997 domain-containing protein -
  HCG48_RS24665 (HCG48_24665) - 6170476..6170862 (+) 387 WP_168571546.1 DUF1257 domain-containing protein -
  HCG48_RS24670 (HCG48_24670) - 6170862..6171326 (+) 465 WP_168571547.1 ferredoxin -
  HCG48_RS24675 (HCG48_24675) - 6171476..6172066 (-) 591 WP_168571548.1 hypothetical protein -
  HCG48_RS24680 (HCG48_24680) - 6172131..6173252 (-) 1122 WP_210437127.1 type II secretion system F family protein -
  HCG48_RS24685 (HCG48_24685) - 6173530..6174627 (-) 1098 WP_168571549.1 type IV pilus twitching motility protein PilT -
  HCG48_RS24690 (HCG48_24690) - 6174669..6174833 (-) 165 WP_168571550.1 hypothetical protein -
  HCG48_RS24695 (HCG48_24695) pilB/pilB1 6174850..6176862 (-) 2013 WP_168571551.1 GspE/PulE family protein Machinery gene
  HCG48_RS24700 (HCG48_24700) - 6176942..6177184 (-) 243 WP_168571552.1 hypothetical protein -
  HCG48_RS24705 (HCG48_24705) gmk 6177492..6178067 (-) 576 WP_168571553.1 guanylate kinase -
  HCG48_RS24710 (HCG48_24710) remA 6178193..6178453 (-) 261 WP_168572067.1 extracellular matrix/biofilm regulator RemA -
  HCG48_RS26975 - 6178631..6178804 (-) 174 Protein_4940 extracellular matrix/biofilm biosynthesis regulator RemA family protein -
  HCG48_RS24720 (HCG48_24720) - 6178997..6180757 (+) 1761 WP_168571554.1 NFACT family protein -
  HCG48_RS24725 (HCG48_24725) dndE 6181010..6181429 (-) 420 WP_168571555.1 DNA sulfur modification protein DndE -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74283.57 Da        Isoelectric Point: 5.0227

>NTDB_id=436512 HCG48_RS24695 WP_168571551.1 6174850..6176862(-) (pilB/pilB1) [Oxynema aestuarii AP17]
MTNFSSQRRALVVQSNFSPFGNKLIQAGYADRDQIQRAQIESRKSGRPLTEVLESITGKQLPPELLRQYKKQQLFELKIL
YGVEAVDPEINEISNEQLAELMENVIALDVCRRHRVIPLSRNDTQPPSLSVAMVDPDKLDALDEINQILRRQGLALRKQV
IAPEDYEQLLKQYSDEQAQKGAAAAAQANKLDLSLNADAFETVDEELDEADDDGDEMNLDAALRGAQEAPIVNTVNKILA
KALSDGVSDIHVEPQEEFLRIRMRKDGVLQEFARLPKKIVPAITSRFKIISDLDIAERRNAQDGRIRRIFQGRTVDFRVN
CLPSRYGEKLCLRILDNSSTQLGLDKLISEPEALAKMREFASRPFGLILVTGPTGSGKSTSLYSVLAERNSPEVNISTAE
DPIEYALPGITQCQVIRAKGLDFEALLRAFMRQDPDIILVGETRDRETAKAAIEAALTGHLVLTTLHTNDAAGAIARLDE
MGVEPFMVAGALIGVSAQRLMRRVCSECREPYHPDKEELARYGLSAAQETNIDFYKARVVSAGERKAAASSGNLCPKCGG
SGYKGRVGVYEILKNTEAVAELITQGAPTERIKEKAVEEGMVSLLPYSINLVRQGMTTFEEVDRVTYTDTGLEAELRAKR
KSALTCKNCGAGLQQEWLDCPYCLTPRFQD

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=436512 HCG48_RS24695 WP_168571551.1 6174850..6176862(-) (pilB/pilB1) [Oxynema aestuarii AP17]
ATGACTAATTTCTCATCGCAGCGACGTGCGCTAGTCGTTCAGAGTAACTTTTCCCCTTTCGGCAACAAACTCATCCAAGC
TGGATATGCCGATCGCGACCAAATCCAGCGAGCGCAAATAGAAAGCCGCAAGTCAGGTCGCCCACTTACAGAAGTTCTAG
AAAGTATCACGGGCAAACAACTTCCACCGGAATTGTTGCGCCAGTACAAAAAACAACAATTATTCGAACTCAAAATCCTT
TACGGCGTCGAAGCCGTCGATCCGGAAATCAACGAGATTTCCAACGAACAGCTCGCCGAGCTGATGGAAAACGTCATCGC
CCTCGACGTTTGCCGTCGTCACCGGGTCATCCCCTTGAGCCGCAACGACACCCAACCCCCATCCCTGTCGGTGGCCATGG
TCGATCCCGACAAACTCGACGCCCTCGACGAAATTAACCAAATCTTGCGGCGGCAAGGACTCGCCCTCAGAAAGCAAGTT
ATCGCCCCGGAAGACTACGAACAACTCCTCAAACAATATTCCGACGAACAAGCTCAAAAAGGCGCGGCAGCCGCCGCTCA
AGCCAACAAGCTCGACCTGTCTCTCAATGCAGACGCCTTTGAAACCGTAGACGAAGAACTCGACGAAGCCGACGACGACG
GCGACGAGATGAACTTGGACGCAGCCTTGCGCGGCGCCCAAGAAGCACCGATCGTCAATACGGTCAACAAAATTCTCGCC
AAAGCCCTTTCCGACGGGGTATCGGACATTCACGTCGAACCGCAAGAAGAATTCCTGCGGATTCGGATGCGCAAAGACGG
GGTACTGCAAGAATTTGCCCGCTTACCGAAGAAAATCGTTCCGGCAATTACGTCTCGATTCAAAATTATCTCGGATTTAG
ATATTGCCGAACGCCGCAATGCCCAAGACGGTCGGATTCGGCGGATTTTCCAAGGACGCACCGTAGACTTTCGGGTCAAC
TGCCTGCCGAGTCGCTACGGCGAAAAACTTTGTTTGCGGATTTTAGACAACTCCAGCACCCAACTCGGTCTCGATAAGTT
GATTAGCGAACCGGAAGCCCTGGCAAAAATGCGCGAGTTTGCCAGTCGTCCCTTCGGACTGATTCTGGTGACCGGGCCGA
CGGGTTCGGGGAAATCGACCTCCCTATACTCGGTGTTGGCGGAACGCAACAGCCCGGAAGTGAATATCAGTACTGCCGAA
GACCCGATCGAATATGCCTTGCCCGGGATTACCCAATGTCAGGTGATTCGGGCGAAAGGATTGGACTTTGAAGCGCTGTT
GCGGGCGTTCATGCGGCAAGACCCGGATATCATTCTGGTGGGTGAGACTCGCGACCGAGAAACGGCCAAAGCGGCGATCG
AGGCAGCCCTGACGGGTCACTTGGTGCTGACGACTTTGCACACGAACGATGCTGCCGGGGCGATCGCCCGTTTGGACGAA
ATGGGCGTCGAACCGTTCATGGTCGCCGGGGCATTGATCGGAGTGTCGGCACAACGCTTGATGCGTCGGGTCTGTAGCGA
ATGCCGCGAACCCTACCACCCGGACAAAGAGGAACTGGCCCGTTATGGCTTGAGTGCCGCTCAAGAAACGAATATCGACT
TTTACAAAGCCCGCGTCGTTTCTGCGGGAGAACGCAAAGCCGCAGCCAGTAGCGGAAACCTCTGTCCTAAATGCGGCGGC
AGTGGTTATAAAGGACGGGTTGGGGTTTACGAAATCCTCAAGAATACCGAAGCGGTTGCCGAGTTGATTACCCAAGGGGC
GCCGACCGAACGGATTAAGGAAAAAGCGGTGGAAGAAGGCATGGTTTCCCTCCTGCCGTACAGTATTAACTTAGTCCGTC
AAGGGATGACTACCTTTGAAGAAGTCGATCGCGTCACCTACACCGACACGGGTCTCGAAGCCGAATTGAGGGCGAAGCGC
AAGAGCGCTTTAACCTGTAAAAATTGTGGAGCGGGACTACAGCAAGAATGGCTCGACTGTCCTTATTGTTTGACCCCGCG
TTTCCAAGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(237-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

64.91

99.104

0.643
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.486

92.985

0.367