Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HF313_RS04650 Genome accession   NZ_CP051166
Coordinates   1024419..1025891 (+) Length   490 a.a.
NCBI ID   WP_126077622.1    Uniprot ID   A0A430HD69
Organism   Massilia atriviolacea strain SOD     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1019419..1030891
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF313_RS04625 (HF313_04645) - 1020827..1021849 (+) 1023 WP_164558072.1 KpsF/GutQ family sugar-phosphate isomerase -
  HF313_RS04630 (HF313_04650) - 1021851..1022393 (+) 543 WP_229427347.1 HAD family hydrolase -
  HF313_RS04635 (HF313_04655) lptC 1022415..1023038 (+) 624 WP_126077619.1 LPS export ABC transporter periplasmic protein LptC -
  HF313_RS04640 (HF313_04660) lptA 1023035..1023661 (+) 627 WP_126077620.1 lipopolysaccharide transport periplasmic protein LptA -
  HF313_RS04645 (HF313_04665) lptB 1023661..1024416 (+) 756 WP_126077621.1 LPS export ABC transporter ATP-binding protein -
  HF313_RS04650 (HF313_04670) rpoN/rpoN1 1024419..1025891 (+) 1473 WP_126077622.1 RNA polymerase factor sigma-54 Machinery gene
  HF313_RS04655 (HF313_04675) - 1026184..1027521 (-) 1338 WP_126077623.1 hypothetical protein -
  HF313_RS04660 (HF313_04680) hpf 1027909..1028274 (+) 366 WP_126077624.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  HF313_RS04665 (HF313_04685) - 1028728..1029450 (+) 723 Protein_908 reverse transcriptase domain-containing protein -
  HF313_RS04670 (HF313_04690) - 1029559..1030644 (+) 1086 WP_126076186.1 enoyl-CoA hydratase/isomerase family protein -

Sequence

Protein


Download         Length: 490 a.a.        Molecular weight: 54395.85 Da        Isoelectric Point: 4.8244

>NTDB_id=436474 HF313_RS04650 WP_126077622.1 1024419..1025891(+) (rpoN/rpoN1) [Massilia atriviolacea strain SOD]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELEQLLSDNPLLERLDDPLDHSVRLLGDGAISSTSTPTEAAPEPG
AQDAPAPSEGEGFEGGEADSPASNDNDGEWGDLGRAKTPDDEDSRPQLEASGITLREHLMEQMRVTVLEARDRALVELIV
DAIDDNGYLTESLEEIHERMPPELDIEIDELRTALALLQSFDPVGVGARNASECLALQIRRMPGVALVTRRMALTIVENH
LTWFAQREFNKLKKALDCDDEDLREAQTVIRQCNPHPGAEFASDVSDYVVPDVIVRKAKNGWSVMLNNDVMPRLRVNSMY
AALLKQGKGEGQMGAQLQEAKWLIKNMRQRFDTILRVAEAIVERQRNFFSHGAVAMRPLVLREIADTLGLHESTISRVTT
QKYMMTPHGMFELKYFFGSHVATEAGGEASSTAIRALIVQLTSAEDPKSPLSDSKIADMLGEQGMVIARRTVAKYREALK
IPPVALRKCL

Nucleotide


Download         Length: 1473 bp        

>NTDB_id=436474 HF313_RS04650 WP_126077622.1 1024419..1025891(+) (rpoN/rpoN1) [Massilia atriviolacea strain SOD]
ATGAAACAGTCCCTTCAACTACGCACCTCCCAGCACCTTGCACTGACGCCCCAGCTGCAGCAATCGATCCGGCTGCTGCA
GTTGTCGACCCTGGAACTGCACCAGGAACTGGAACAGCTGTTGAGCGACAACCCGCTGCTCGAACGGCTCGACGATCCGC
TCGATCATTCGGTGCGGCTGCTGGGCGATGGCGCCATCAGCAGCACCTCGACCCCGACCGAAGCGGCGCCCGAGCCGGGT
GCGCAGGACGCCCCGGCCCCAAGCGAAGGCGAAGGCTTCGAAGGCGGCGAGGCGGACAGCCCCGCCAGCAACGACAACGA
CGGCGAGTGGGGCGACCTCGGTCGCGCCAAGACGCCCGACGACGAGGATTCGCGTCCGCAGCTCGAAGCGTCCGGGATCA
CCCTGCGCGAGCACCTGATGGAGCAGATGCGCGTGACCGTGCTCGAAGCGCGCGACCGTGCCCTGGTCGAACTGATCGTC
GACGCCATCGACGACAACGGCTACCTGACCGAATCGCTGGAAGAGATCCACGAGCGCATGCCGCCCGAGCTGGACATCGA
GATCGACGAACTACGCACCGCGCTGGCGCTGCTGCAAAGCTTCGACCCGGTCGGGGTGGGTGCGCGCAACGCGTCCGAAT
GCCTGGCGCTGCAGATCCGGCGCATGCCGGGCGTGGCGCTGGTCACGCGGCGCATGGCGCTGACCATCGTCGAAAACCAC
CTGACCTGGTTTGCCCAGCGCGAATTCAACAAGCTGAAAAAAGCGCTCGACTGCGACGATGAAGATTTGCGCGAGGCGCA
GACCGTGATCCGCCAGTGCAATCCGCATCCGGGCGCCGAGTTCGCGTCCGACGTATCGGACTACGTGGTGCCGGACGTAA
TCGTGCGCAAGGCGAAGAATGGATGGTCGGTGATGCTCAATAACGACGTCATGCCGCGCCTGCGCGTAAACAGCATGTAC
GCGGCGCTGCTCAAGCAGGGCAAGGGCGAAGGGCAGATGGGCGCGCAGCTGCAGGAAGCCAAATGGCTGATCAAGAACAT
GCGCCAGCGCTTCGACACCATCCTGCGGGTAGCCGAAGCGATCGTCGAACGCCAGCGCAATTTCTTCTCGCACGGCGCGG
TGGCGATGCGTCCGCTGGTGCTGCGCGAAATCGCCGACACGCTCGGCCTGCACGAAAGTACGATATCGCGCGTGACCACG
CAAAAGTACATGATGACGCCGCACGGCATGTTCGAGCTGAAGTATTTTTTCGGCAGCCACGTGGCCACCGAAGCGGGCGG
CGAAGCCTCGTCGACCGCGATCCGCGCGCTGATCGTGCAGCTGACCAGCGCCGAAGACCCGAAGAGTCCTCTGTCGGACA
GCAAAATCGCCGACATGCTGGGCGAGCAGGGCATGGTGATTGCGCGCCGCACGGTCGCCAAATACCGCGAAGCGCTCAAG
ATCCCGCCGGTCGCCCTGCGCAAGTGCCTCTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-320)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(335-488)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A430HD69

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

57.654

100

0.592