Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HF500_RS02665 Genome accession   NZ_CP051162
Coordinates   552381..553754 (+) Length   457 a.a.
NCBI ID   WP_063167015.1    Uniprot ID   -
Organism   Geobacillus subterraneus strain CPW16     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 547381..558754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF500_RS02645 (HF500_02645) ctsR 547755..548216 (+) 462 WP_063167019.1 transcriptional regulator CtsR -
  HF500_RS02650 (HF500_02650) - 548231..548779 (+) 549 WP_063167018.1 UvrB/UvrC motif-containing protein -
  HF500_RS02655 (HF500_02655) - 548784..549875 (+) 1092 WP_063167017.1 protein arginine kinase -
  HF500_RS02660 (HF500_02660) clpC 549872..552307 (+) 2436 WP_063167016.1 ATP-dependent protease ATP-binding subunit ClpC -
  HF500_RS02665 (HF500_02665) radA 552381..553754 (+) 1374 WP_063167015.1 DNA repair protein RadA Machinery gene
  HF500_RS02670 (HF500_02670) - 553962..555056 (+) 1095 WP_063167014.1 PIN/TRAM domain-containing protein -
  HF500_RS02675 (HF500_02675) ispD 555078..555764 (+) 687 WP_063167013.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  HF500_RS02680 (HF500_02680) ispF 555779..556261 (+) 483 WP_063167012.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  HF500_RS02685 (HF500_02685) gltX 556489..557958 (+) 1470 WP_168368003.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49260.76 Da        Isoelectric Point: 7.7341

>NTDB_id=436398 HF500_RS02665 WP_063167015.1 552381..553754(+) (radA) [Geobacillus subterraneus strain CPW16]
MVKKKTKFVCQECGYESAKWLGRCPGCQTWNSFVEEIERAKPAARGAFLHSEPSGPAKPVPITAVTAAQEPRIETNSAEL
NRVLGGGIVKGSLVLIGGDPGIGKSTLLLQTSARLAAAGHTVLYISGEESVKQVKLRAGRLHAESNQLYVLAEADLEYIV
AAIETIQPACVIVDSIQTVYRTDITSAPGSVAQVRECTAELMKIAKTKGIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYF
EGERHHTYRILRAVKNRFGSTNEIGIFEMRDIGLREVENPSEVFLEERSRGAAGSTVVAAMEGTRPVLVEIQALVSPTSF
GNPRRMATGLDHSRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDRPTNPADVIIGEVGLT
GEVRRVSRIEQRVQEAVKLGFSRVIVPNNNLTGWQPPAGVNVIGVSHVAEALEHTML

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=436398 HF500_RS02665 WP_063167015.1 552381..553754(+) (radA) [Geobacillus subterraneus strain CPW16]
ATGGTGAAAAAGAAAACGAAATTTGTTTGCCAAGAATGTGGGTATGAATCAGCCAAATGGCTCGGCCGCTGTCCGGGCTG
CCAAACGTGGAATTCGTTCGTTGAAGAAATTGAGCGAGCGAAACCGGCTGCGCGCGGGGCGTTTCTTCATTCTGAGCCAT
CGGGGCCGGCCAAACCGGTTCCGATTACAGCCGTTACGGCGGCGCAAGAGCCGCGCATTGAAACGAACAGTGCGGAGCTC
AATCGCGTGCTCGGCGGGGGAATCGTCAAAGGCTCGCTTGTCTTAATTGGCGGCGATCCCGGCATCGGCAAGTCGACGTT
ACTGCTGCAAACATCAGCGCGGCTCGCCGCGGCCGGACATACGGTGTTGTATATATCCGGTGAGGAATCGGTGAAGCAAG
TGAAGCTGCGCGCCGGGCGCCTCCATGCCGAGTCCAATCAACTGTATGTGTTAGCGGAGGCGGATTTGGAATATATTGTA
GCAGCGATTGAAACGATTCAGCCTGCCTGTGTGATTGTCGATTCCATTCAGACGGTGTACCGGACGGATATTACGTCGGC
GCCGGGCAGCGTCGCCCAAGTGCGCGAGTGCACCGCTGAGCTGATGAAAATCGCCAAGACGAAAGGCATCGCGATTTTCA
TTGTCGGCCATGTGACGAAAGAAGGGGCGATCGCCGGGCCGCGTCTGCTCGAGCATATGGTCGATACGGTTCTTTATTTT
GAAGGGGAACGGCATCATACGTACCGCATTTTGCGGGCGGTGAAAAACCGCTTCGGTTCGACGAATGAAATCGGCATTTT
TGAAATGCGTGACATCGGGCTGCGAGAGGTGGAAAACCCGTCGGAAGTGTTTTTGGAAGAGCGGTCGCGCGGGGCGGCTG
GCTCGACGGTTGTCGCGGCGATGGAAGGGACGCGCCCGGTGCTCGTTGAAATTCAGGCGCTCGTTTCGCCGACGAGTTTC
GGCAATCCAAGGCGGATGGCGACCGGCCTCGACCATAGCCGCGTATCGCTGCTCATGGCCGTGCTTGAAAAGCGGGTCGG
TCTGCTGTTGCAAAACCAGGACGCCTATTTGAAGGTGGCTGGCGGGGTAAAGCTTGACGAACCGGCCATCGATTTGGCGG
TCGCTGTCAGCATCGCCTCGAGTTTCCGCGATCGGCCAACCAACCCGGCCGATGTCATTATCGGTGAGGTCGGCTTGACC
GGGGAGGTGCGCCGTGTTTCCCGCATTGAACAACGCGTGCAAGAGGCTGTCAAATTAGGGTTTTCCCGCGTCATTGTGCC
GAACAACAATTTGACTGGCTGGCAGCCGCCTGCAGGCGTCAACGTGATCGGTGTTTCGCATGTGGCCGAGGCGCTCGAGC
ATACGATGTTATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(59-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.179

100

0.742
  radA Streptococcus mitis SK321

64.459

99.125

0.639
  radA Streptococcus mitis NCTC 12261

64.097

99.344

0.637
  radA Streptococcus pneumoniae Rx1

64.238

99.125

0.637
  radA Streptococcus pneumoniae D39

64.238

99.125

0.637
  radA Streptococcus pneumoniae R6

64.238

99.125

0.637
  radA Streptococcus pneumoniae TIGR4

64.238

99.125

0.637