Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HF520_RS13605 Genome accession   NZ_CP051144
Coordinates   2807789..2809162 (+) Length   457 a.a.
NCBI ID   WP_168574454.1    Uniprot ID   -
Organism   Romboutsia sp. CE17     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2802789..2814162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF520_RS13595 (HF520_13595) - 2803150..2805078 (+) 1929 WP_168574452.1 elongation factor G -
  HF520_RS13600 (HF520_13600) - 2805204..2807657 (+) 2454 WP_168574453.1 ATP-dependent Clp protease ATP-binding subunit -
  HF520_RS13605 (HF520_13605) radA 2807789..2809162 (+) 1374 WP_168574454.1 DNA repair protein RadA Machinery gene
  HF520_RS13610 (HF520_13610) disA 2809165..2810235 (+) 1071 WP_168574455.1 DNA integrity scanning diadenylate cyclase DisA -
  HF520_RS13615 (HF520_13615) - 2810321..2811415 (+) 1095 WP_168574456.1 PIN/TRAM domain-containing protein -
  HF520_RS13620 (HF520_13620) ispD 2811561..2812247 (+) 687 WP_168574457.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  HF520_RS13625 (HF520_13625) ispF 2812275..2812754 (+) 480 WP_168574458.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49999.93 Da        Isoelectric Point: 8.2891

>NTDB_id=436204 HF520_RS13605 WP_168574454.1 2807789..2809162(+) (radA) [Romboutsia sp. CE17]
MAKIRTKYVCQGCGYETSKWLGKCPECTKWNTFVEEIEQKNSKKEVFIIDKSSSRPININAIETKEEERFSTEIAELDRV
LGGGIVKGSLVLVGGDPGIGKSTLLIQVSSNVANAGKKVLYISGEESESQIKMRAKRLGINSDNLYIFAENNLSIIEANL
DSINPELIILDSIQTVYSPEIASAPGTVSQIKEGTSKFMKISKKMGISTFVVGHVTKEGSLAGPKLLEHMVDTVIYFEGE
RYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDNPSKVLISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIA
KRTATGVDFNRVALLLAVLEKRVGLQIQNQDVYINVVGGIKINEPSIDMGIIIAVASSFRNIPVSEDVVVTGEVGLTGEV
RAVSFIEKRIAECKKLGFKKMVIPRNNYDAVKEIKGIDIIPVDNLRQAINIVLGGNK

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=436204 HF520_RS13605 WP_168574454.1 2807789..2809162(+) (radA) [Romboutsia sp. CE17]
ATGGCAAAAATTAGAACAAAATATGTATGTCAGGGATGTGGATATGAAACTAGTAAATGGCTAGGGAAATGTCCAGAATG
TACAAAATGGAATACATTCGTAGAAGAGATAGAGCAGAAAAATTCAAAAAAGGAAGTTTTTATAATAGATAAGTCTTCTT
CAAGACCAATAAATATAAATGCTATAGAAACAAAGGAAGAAGAAAGGTTTTCTACTGAAATAGCAGAGCTAGATAGAGTT
CTTGGAGGTGGGATAGTAAAAGGGTCATTGGTTCTTGTAGGAGGAGACCCTGGAATAGGAAAATCCACACTTTTAATACA
AGTATCAAGCAATGTAGCTAATGCAGGAAAAAAAGTTTTATATATATCGGGAGAAGAATCTGAATCTCAAATAAAAATGA
GAGCTAAAAGATTAGGTATTAATTCCGATAATTTATATATATTCGCAGAAAATAATTTAAGTATAATAGAAGCAAATTTA
GATAGTATAAATCCAGAGCTTATAATACTTGACTCTATACAAACCGTATATAGCCCAGAAATAGCTTCAGCACCAGGTAC
TGTAAGCCAAATTAAAGAGGGAACTTCTAAATTTATGAAAATTTCCAAAAAAATGGGTATATCTACTTTTGTAGTTGGAC
ATGTAACAAAAGAGGGATCTTTAGCAGGGCCAAAGTTGTTAGAGCATATGGTGGATACTGTTATATACTTTGAAGGAGAA
AGATATAACACATATAGATTAGTTAGAGCCGTTAAAAATAGATTTGGATCTACTAATGAGTTAGGTGTGTTTGAAATGAG
AGATTTAGGTTTAGTAGAACTTGATAATCCATCAAAAGTGCTTATATCAGAAAAACCAAAAGATGTAGCAGGGTCAGTCA
TAATTTCTACTGTTGAAGGAACTAGACCAATGCTACTTGAGCTTCAAGCTTTGGTTTCACCTACTAGCTTTGGAATAGCA
AAGAGAACTGCTACAGGTGTTGATTTTAACAGAGTAGCTTTACTTTTAGCAGTACTAGAAAAGCGTGTAGGGCTTCAAAT
ACAAAATCAAGATGTATATATTAATGTAGTTGGAGGAATTAAAATAAATGAACCTTCTATAGACATGGGGATAATAATTG
CAGTAGCATCAAGCTTTAGAAATATTCCGGTATCAGAGGATGTTGTTGTTACAGGTGAGGTTGGATTAACAGGAGAAGTA
AGAGCTGTTAGTTTTATAGAGAAAAGGATAGCTGAATGTAAAAAACTTGGATTTAAGAAAATGGTTATTCCTAGAAATAA
CTATGATGCAGTAAAAGAAATAAAAGGGATAGATATTATTCCAGTAGATAATTTAAGGCAAGCAATCAATATAGTGCTAG
GGGGGAATAAATAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(65-219)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(348-452)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

56.55

100

0.567
  radA Streptococcus pneumoniae Rx1

51.101

99.344

0.508
  radA Streptococcus pneumoniae D39

51.101

99.344

0.508
  radA Streptococcus pneumoniae R6

51.101

99.344

0.508
  radA Streptococcus pneumoniae TIGR4

51.101

99.344

0.508
  radA Streptococcus mitis NCTC 12261

50.881

99.344

0.505
  radA Streptococcus mitis SK321

50.881

99.344

0.505