Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoS   Type   Regulator
Locus tag   IIE08_RS14740 Genome accession   NZ_CP062985
Coordinates   3028743..3029735 (-) Length   330 a.a.
NCBI ID   WP_000081550.1    Uniprot ID   A0A370V765
Organism   Escherichia coli UTI89     
Function   regulation of chitinases (predicted from homology)   
Competence regulation

Genomic Context

Location: 3023743..3034735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IIE08_RS14720 ygbM 3024374..3025150 (+) 777 WP_001136908.1 HPr family phosphocarrier protein -
  IIE08_RS14725 ygbN 3025239..3026603 (+) 1365 WP_000104443.1 GntP family transporter -
  IIE08_RS14730 - 3026746..3027789 (-) 1044 WP_001161578.1 MBL fold metallo-hydrolase -
  IIE08_RS14735 - 3028014..3028565 (+) 552 WP_001179442.1 flavodoxin family protein -
  IIE08_RS14740 rpoS 3028743..3029735 (-) 993 WP_000081550.1 RNA polymerase sigma factor RpoS Regulator
  IIE08_RS14745 nlpD 3029798..3030892 (-) 1095 WP_306305124.1 murein hydrolase activator NlpD -
  IIE08_RS14750 pcm 3031077..3031703 (-) 627 WP_000254701.1 protein-L-isoaspartate O-methyltransferase -
  IIE08_RS14755 surE 3031697..3032458 (-) 762 WP_001295182.1 5'/3'-nucleotidase SurE -
  IIE08_RS14760 truD 3032439..3033488 (-) 1050 WP_000568928.1 tRNA pseudouridine(13) synthase TruD -
  IIE08_RS14765 ispF 3033485..3033964 (-) 480 WP_001219247.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  IIE08_RS14770 ispD 3033964..3034674 (-) 711 WP_000246149.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 37972.84 Da        Isoelectric Point: 4.5597

>NTDB_id=436057 IIE08_RS14740 WP_000081550.1 3028743..3029735(-) (rpoS) [Escherichia coli UTI89]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide


Download         Length: 993 bp        

>NTDB_id=436057 IIE08_RS14740 WP_000081550.1 3028743..3029735(-) (rpoS) [Escherichia coli UTI89]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGCTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTGATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATCGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCACGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAATGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTACTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAATAA

Domains

Predicted by InterProScan.

Sigma-70 region 3 (Sigma70_r3)

(174-248)

Sigma-70 region 2 (Sigma70_r2)

(94-163)

Sigma-70 factor, region 1.2 (Sigma70_r1_2)

(56-88)

Sigma-70, region 4 (Sigma70_r4)

(262-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A370V765

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoS Vibrio cholerae O1 biovar El Tor strain E7946

73.065

97.879

0.715