Detailed information NTG0436055

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: HCM98_RS05785
• Genome accession: NZ_CP051013
• Coordinates: 1109396..1113121 (+)
• Length: 1241 a.a.
• NCBI ID: WP_029437815.1
• Uniprot ID: -
• Organism: Bacillus tropicus strain LM1212-DB
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1104396..1118121

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HCM98_RS05770 (HCM98_05775)	-	1104554..1105147 (+)	594	WP_000347516.1	TVP38/TMEM64 family protein	-
HCM98_RS05775 (HCM98_05780)	lepB	1105204..1105767 (+)	564	WP_000751904.1	signal peptidase I	-
HCM98_RS05780 (HCM98_05785)	addB	1105884..1109399 (+)	3516	WP_029437814.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
HCM98_RS05785 (HCM98_05790)	addA	1109396..1113121 (+)	3726	WP_029437815.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
HCM98_RS05790 (HCM98_05795)	-	1113134..1113421 (+)	288	WP_029437816.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
HCM98_RS05795 (HCM98_05800)	gerPF	1113527..1113742 (-)	216	WP_001141566.1	spore germination protein GerPF	-
HCM98_RS05800 (HCM98_05805)	-	1113785..1114171 (-)	387	WP_029437817.1	spore germination protein GerPE	-
HCM98_RS05805 (HCM98_05810)	gerPD	1114187..1114381 (-)	195	WP_001102341.1	spore germination protein GerPD	-
HCM98_RS05810 (HCM98_05815)	gerPC	1114388..1115002 (-)	615	WP_029437818.1	spore germination protein GerPC	-
HCM98_RS05815 (HCM98_05820)	gerPB	1115071..1115277 (-)	207	WP_001012508.1	spore germination protein GerPB	-
HCM98_RS05820 (HCM98_05825)	gerPA	1115292..1115513 (-)	222	WP_001111187.1	spore germination protein GerPA	-
HCM98_RS05825 (HCM98_05830)	-	1115610..1115789 (-)	180	WP_029437819.1	aspartyl-phosphate phosphatase Spo0E family protein	-
HCM98_RS05830 (HCM98_05835)	-	1116037..1116936 (+)	900	WP_029437820.1	fumarylacetoacetate hydrolase family protein	-
HCM98_RS05835 (HCM98_05840)	-	1116972..1117256 (-)	285	WP_000925339.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142861.27 Da
• Isoelectric Point: 4.8691

>NTDB_id=436055 HCM98_RS05785 WP_029437815.1 1109396..1113121(+) (addA) [Bacillus tropicus strain LM1212-DB]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADLVLLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQTMKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPDGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKVMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSLFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEIHLELGQGSIPDEIYGYSASWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATAHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIRTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSEETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EERYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=436055 HCM98_RS05785 WP_029437815.1 1109396..1113121(+) (addA) [Bacillus tropicus strain LM1212-DB]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCCGTTGTAGCGAATGGGCG
TGATATTTTAGTCGCAGCAGCAGCTGGATCAGGGAAAACAGCGGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGAGCCAGGTTCTCAGCACATTCGAAAGCAGCTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCCTTTTGTTTACAAGTTATTAGAGGATACTATTATATGCTTGATGTTGATCCTCGTTTCC
GCATAGCGAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATAGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGACGATGATTTACAACGTATGATTTTAGC
ACTTCATACAGAATCAAGAGCACATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATACGATGTGGAAGGAA
AGACAATTGAAGATTTAGTGTACGCTTCCTACTTATTAGAAGATGTGAAATTCCAACTTGAAACAGCGGAACAGCATATT
CGTAAAGCGACAGAGCTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCGTTGAAACCCTGCAAGCAGATTTAGTTTTACT
TGGAACGTTATCATCAGCTGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAGCAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGCAAGCCTGAAAGTTTCTTGCGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTTGTACAGCTTGTAAAAGTATTTACAGAACGTTTCCAAACGATGAAGCGAGATAAAGGAATGGTCGATTTCACGG
ATTTAGAGCATTTTTGTTTACAAATTTTAAGTGAACAAAGTGAAGATGGTGAAATGAAGCCATCAGCAGTAGCGCTTCAA
TATCGCAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCGATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGCGACGTGAAGCAGTCAATCTATCGTTTCCGACTAGCAG
AACCAGGATTATTCCTAGGAAAGTATAAACGCTTCACGCAAGAAGGATTAGGCGGCGGAATGAAGATCGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCAGGCACGAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
CGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTACCTATCCAGATGGTGAAGATGTAGCAGCTGAGCTACTATGCA
TTCAGCAAACGGAAGAAGAGGTAATAGACGGTGAAGAAGGTGCGGAAGTAGAAAAGGCGCAGCTTGAAGCTCGTCTTATG
GCGCAACGCATTAAAGTGATGGTCGATTCTGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGCGACTTCGTTATTTTACTCCGCTCCATGCCGTGGGCACCGCAAATTATGGAAGAGTTGAAATTACAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCAGTACTTCGTTCACCAATCGTTGGATTAAACGATGAAGAACTTGCAACGCTTCG
TGCTCACGGGAAGAAAGGTTCGTTTTATGAAGTAATGAGCTTATTCTTAAAAGGGGCACCGCTTGAAGAAGAAAAAGAAC
TACATGATAAATTAGAATGGTTCTACAACTTACTGCAAGGATGGCGTGAATTCGCGCGCCAACAGTCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTATGATTTTGTCGGTGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGCCTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAGCGCGGTGATGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGCATTATGACAATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTCGTAGCTGGACTAGGTCGCCGCTTTAATACACAAGACTTAATGAA
GCGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCGCAATTTATTGATCCACGTAAACGAATTAAATATACGACATTAT
CGCAACTTGCGATTAAGCGTAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGCGTATTATATGTTGCGTTAACACGT
GCAAAAGAGAAGTTAATTTTAATTGGAACGGTTAAGGATGCAAATAAAGAAATGGAAAAATGGCTTGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGAGCGTCTTGCTACTTAGACTGGATTGCACCTTCCTTATATAGAC
ATCGTGATAGTGAAATACATCTTGAATTAGGGCAAGGAAGCATTCCAGATGAAATTTATGGATATAGTGCGAGCTGGAAA
GTAGAAGTTGTTGACGGTAACACGTTACTTGCGCCAGAACCCGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCGTTGCAAAGTGAACGAAAAGAAGAGGTGTACGATAGATTAATGTGGAAGTACGGATATGAGG
AAGCGACAGCTCATCGTGCGAAGCAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAATTACGTGCACCAATTAGAACCCGTCCTCGTTTTATGGAGAAAAAAGGTTTAACGTACGCAGAGCGTGG
AACTGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGGTTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGTGAAGAAACGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTCCCAGGCGGATTCGAACAAGCGAAACCAATTTTA
GAAGAGCGATATAAAGTGCAGCTTTCGTTATATGCAAAAGCGCTCGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGTAATCATGTTGTAAAAATCGAAGAATAG

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1059-1231)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.778	100	0.539