Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   GRB95_RS01960 Genome accession   NZ_CP051004
Coordinates   347782..349071 (+) Length   429 a.a.
NCBI ID   WP_000432954.1    Uniprot ID   A0AAW6XXN4
Organism   Streptococcus agalactiae 515     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 342782..354071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GRB95_RS01940 (GRB95_01940) - 344089..344757 (+) 669 WP_000394891.1 fructose-6-phosphate aldolase -
  GRB95_RS01945 (GRB95_01945) - 344825..345913 (+) 1089 WP_000862123.1 glycerol dehydrogenase -
  GRB95_RS01950 (GRB95_01950) cysK 346065..346991 (-) 927 WP_000036944.1 cysteine synthase A -
  GRB95_RS01955 (GRB95_01955) - 347082..347726 (-) 645 WP_001108144.1 YigZ family protein -
  GRB95_RS01960 (GRB95_01960) comFA/cflA 347782..349071 (+) 1290 WP_000432954.1 DEAD/DEAH box helicase Machinery gene
  GRB95_RS01965 (GRB95_01965) - 349071..349736 (+) 666 WP_000128301.1 ComF family protein -
  GRB95_RS01970 (GRB95_01970) raiA 349813..350367 (+) 555 WP_000599096.1 ribosome-associated translation inhibitor RaiA -

Sequence

Protein


Download         Length: 429 a.a.        Molecular weight: 48730.56 Da        Isoelectric Point: 9.9941

>NTDB_id=435889 GRB95_RS01960 WP_000432954.1 347782..349071(+) (comFA/cflA) [Streptococcus agalactiae 515]
MENYLGRLWTKAQLSEQLRKIAINLPSFIKKGSDYICTRCSSSVAKNCQLPTGNYYCRECIVFGRVTSNENLYYFPQKTF
SKTNSLKWKGGLTPYQNEVSEELLKGISSKENLLVHAVTGAGKTEMIYHSIAKVIDTGGSVCIASPRIDVCLELHKRLSN
DFRCAITLMHGESPSYQRSPLTIATTHQLLKFYHAFDLLIVDEVDAFPYVDNPILYHGVKQALKENGTSIFLTATSTTEL
ERKVARKELKKLHLARRFHANPLVIPEMVWVSGIQKSLQTQKLPPKLYQLINKQRQTRYPLLLFFPHISEGQVFTEMLRQ
AFPMEKIGFVSSKSTSRLKLVQDFRDNKLSILVSTTILERGVTFPSVDVFVIQANHHLFTKSSLVQISGRVGRALERPEG
LLYFLHDGKSKSMHQAIKEIKNMNHIGGF

Nucleotide


Download         Length: 1290 bp        

>NTDB_id=435889 GRB95_RS01960 WP_000432954.1 347782..349071(+) (comFA/cflA) [Streptococcus agalactiae 515]
ATGGAAAATTATTTAGGTAGACTTTGGACAAAAGCACAATTATCTGAGCAACTTAGAAAAATTGCAATTAACTTGCCATC
TTTTATAAAGAAAGGAAGCGACTATATTTGTACTCGATGCTCTAGTTCTGTTGCCAAAAATTGCCAATTACCAACTGGAA
ATTATTATTGTAGAGAATGTATAGTTTTTGGAAGAGTTACTAGTAATGAAAATTTATATTATTTCCCACAAAAAACATTT
TCTAAAACGAATTCTTTAAAATGGAAAGGGGGACTGACACCTTATCAAAATGAGGTTTCAGAAGAGTTGTTGAAAGGTAT
TTCAAGTAAGGAAAATCTTCTTGTTCATGCTGTGACAGGGGCTGGTAAAACAGAAATGATATACCACTCTATCGCCAAAG
TAATTGATACTGGAGGAAGCGTTTGTATTGCCAGTCCTAGAATTGATGTTTGTTTAGAATTGCATAAGCGACTTTCAAAC
GATTTTAGATGTGCCATAACATTAATGCATGGAGAATCTCCTTCTTATCAGAGATCCCCTTTAACTATTGCCACAACACA
TCAATTGTTAAAATTTTATCATGCTTTTGATTTGCTAATTGTTGATGAAGTTGATGCTTTTCCATATGTCGACAACCCGA
TACTATACCATGGTGTAAAACAGGCCTTAAAGGAAAACGGAACAAGTATTTTTCTGACTGCAACTTCAACGACTGAGTTA
GAAAGAAAAGTTGCAAGAAAAGAACTAAAAAAATTACATCTAGCTAGGAGATTTCATGCCAATCCGTTAGTGATACCCGA
GATGGTTTGGGTAAGTGGTATCCAGAAAAGCTTGCAGACACAAAAACTACCTCCCAAATTATATCAGCTGATAAATAAAC
AGCGACAAACGAGATATCCTTTGTTATTATTTTTCCCCCATATTTCAGAAGGTCAGGTATTCACCGAAATGTTAAGACAA
GCCTTTCCTATGGAAAAAATTGGTTTTGTTTCAAGTAAGAGCACATCTAGGTTAAAGTTAGTGCAAGATTTTCGAGATAA
TAAATTGTCTATCCTAGTGTCAACTACCATTCTAGAGAGAGGAGTAACTTTCCCTAGCGTCGACGTTTTTGTTATACAAG
CAAATCATCATTTGTTTACGAAAAGTTCCTTGGTACAAATTTCAGGAAGAGTTGGAAGAGCATTAGAAAGGCCCGAAGGG
CTCCTTTACTTCCTTCATGATGGGAAAAGTAAATCGATGCACCAAGCTATAAAGGAGATTAAAAACATGAATCATATAGG
AGGATTTTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(286-394)

DEAD/DEAH box helicase (DEAD)

(93-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

60.422

99.534

0.601
  comFA/cflA Streptococcus pneumoniae Rx1

59.953

99.534

0.597
  comFA/cflA Streptococcus pneumoniae D39

59.953

99.534

0.597
  comFA/cflA Streptococcus pneumoniae R6

59.953

99.534

0.597
  comFA/cflA Streptococcus pneumoniae TIGR4

59.953

99.534

0.597
  comFA/cflA Streptococcus mitis SK321

59.953

99.534

0.597
  comFA Lactococcus lactis subsp. cremoris KW2

50.254

91.841

0.462
  comFA Latilactobacillus sakei subsp. sakei 23K

37.037

100

0.373
  comFA Bacillus subtilis subsp. subtilis str. 168

39.312

94.872

0.373