Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IMP23_RS03110 Genome accession   NZ_CP062908
Coordinates   426700..427377 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain Res13-Lact-EB12-05     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 421700..432377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMP23_RS03095 (IMP23_03095) chiA 423067..425760 (+) 2694 WP_061350742.1 bifunctional chitinase/lysozyme -
  IMP23_RS03100 (IMP23_03100) bfd 425929..426123 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  IMP23_RS03105 (IMP23_03105) bfr 426195..426671 (+) 477 WP_000675504.1 bacterioferritin -
  IMP23_RS03110 (IMP23_03110) pilD 426700..427377 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  IMP23_RS03115 (IMP23_03115) gspM 427377..427838 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  IMP23_RS03120 (IMP23_03120) gspL 427835..428998 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  IMP23_RS03125 (IMP23_03125) gspK 429013..429996 (-) 984 WP_061350741.1 type II secretion system minor pseudopilin GspK -
  IMP23_RS03130 (IMP23_03130) gspJ 429989..430576 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  IMP23_RS03135 (IMP23_03135) gspI 430569..430946 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  IMP23_RS03140 (IMP23_03140) gspH 430943..431452 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  IMP23_RS03145 (IMP23_03145) gspG 431460..431897 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=435644 IMP23_RS03110 WP_000178161.1 426700..427377(-) (pilD) [Escherichia coli strain Res13-Lact-EB12-05]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=435644 IMP23_RS03110 WP_000178161.1 426700..427377(-) (pilD) [Escherichia coli strain Res13-Lact-EB12-05]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378