Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IMP69_RS16800 Genome accession   NZ_CP062876
Coordinates   3452327..3453004 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli O157:H16 strain Res13-Lact-PEA12-26     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3447327..3458004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMP69_RS16775 (IMP69_16780) - 3447917..3448246 (+) 330 Protein_3321 hypothetical protein -
  IMP69_RS16785 (IMP69_16790) - 3449300..3451387 (+) 2088 WP_251122261.1 carbohydrate-binding protein -
  IMP69_RS16790 (IMP69_16795) bfd 3451556..3451750 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  IMP69_RS16795 (IMP69_16800) bfr 3451822..3452298 (+) 477 WP_000675504.1 bacterioferritin -
  IMP69_RS16800 (IMP69_16805) pilD 3452327..3453004 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  IMP69_RS16805 (IMP69_16810) gspM 3453004..3453465 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  IMP69_RS16810 (IMP69_16815) gspL 3453462..3454625 (-) 1164 WP_001115244.1 type II secretion system protein GspL -
  IMP69_RS16815 (IMP69_16820) gspK 3454640..3455623 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  IMP69_RS16820 (IMP69_16825) gspJ 3455616..3456203 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  IMP69_RS16825 (IMP69_16830) gspI 3456196..3456573 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  IMP69_RS16830 (IMP69_16835) gspH 3456570..3457079 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  IMP69_RS16835 (IMP69_16840) gspG 3457087..3457524 (-) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=435290 IMP69_RS16800 WP_000178161.1 3452327..3453004(-) (pilD) [Escherichia coli O157:H16 strain Res13-Lact-PEA12-26]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=435290 IMP69_RS16800 WP_000178161.1 3452327..3453004(-) (pilD) [Escherichia coli O157:H16 strain Res13-Lact-PEA12-26]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378