Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   IMP99_RS08900 Genome accession   NZ_CP062855
Coordinates   1865993..1867417 (-) Length   474 a.a.
NCBI ID   WP_195686825.1    Uniprot ID   -
Organism   Escherichia coli O20:H12 strain Res13-Lact-PEB17-18     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1860993..1872417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMP99_RS08870 (IMP99_08865) yeeZ 1861048..1861872 (+) 825 WP_000754749.1 SDR family oxidoreductase -
  IMP99_RS08875 (IMP99_08870) yeeY 1861918..1862847 (+) 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
  IMP99_RS08880 (IMP99_08875) yoeI 1863062..1863124 (+) 63 WP_010723108.1 membrane protein YoeI -
  IMP99_RS08885 (IMP99_08880) plaP 1863114..1864472 (+) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  IMP99_RS08890 (IMP99_08885) tsuA 1864651..1865709 (+) 1059 WP_061350760.1 thiosulfate utilization transporter TsuA/YeeE -
  IMP99_RS08895 (IMP99_08890) tsuB 1865723..1865950 (+) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  IMP99_RS08900 (IMP99_08895) sbcB 1865993..1867417 (-) 1425 WP_195686825.1 exodeoxyribonuclease I Machinery gene
  IMP99_RS08905 (IMP99_08900) - 1867599..1868777 (+) 1179 WP_195686826.1 site-specific integrase -
  IMP99_RS08910 (IMP99_08905) - 1868758..1868949 (-) 192 WP_000132739.1 AlpA family phage regulatory protein -
  IMP99_RS08915 (IMP99_08910) - 1869027..1869371 (-) 345 WP_032084204.1 hypothetical protein -
  IMP99_RS08920 (IMP99_08915) - 1869398..1869565 (-) 168 WP_195686827.1 hypothetical protein -
  IMP99_RS08925 (IMP99_08920) - 1869654..1869935 (-) 282 WP_000026224.1 hypothetical protein -
  IMP99_RS08930 (IMP99_08925) - 1870127..1870810 (-) 684 WP_195686828.1 ead/Ea22-like family protein -
  IMP99_RS08935 (IMP99_08930) - 1870807..1870974 (-) 168 WP_195686829.1 DUF2737 family protein -
  IMP99_RS08940 (IMP99_08935) - 1870985..1871281 (-) 297 WP_001111275.1 phage anti-RecBCD protein -
  IMP99_RS08945 (IMP99_08940) ssb 1871784..1872257 (-) 474 WP_089075571.1 single-stranded DNA-binding protein -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 54311.54 Da        Isoelectric Point: 5.4682

>NTDB_id=434877 IMP99_RS08900 WP_195686825.1 1865993..1867417(-) (sbcB) [Escherichia coli O20:H12 strain Res13-Lact-PEB17-18]
MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENE
AAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWRG
NTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDAD
RLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETGPRNLPALDITFVDK
RIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDREKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=434877 IMP99_RS08900 WP_195686825.1 1865993..1867417(-) (sbcB) [Escherichia coli O20:H12 strain Res13-Lact-PEB17-18]
ATGACAGACACAGATAAACAACCTACCTTCCTCTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCC
TGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCTG
ATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAA
GCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGA
CGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCT
GGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGTCTA
CCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTA
CGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAAC
ACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGCGGC
AATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCTGTAATTATGGTGGATTTGGCAGGAGACAT
TTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCG
CCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGAC
CGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGT
GGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACG
CCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGGGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTCGATAAA
CGGATTGAAAAGCTGTTGTTCAATTATCGGGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCT
GGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTACAGGGTTATGCAGATGAATTGCAGATGCTGGCACAACAATATG
CCGATGACAGAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(12-187)

Exonuclease C-terminal (ExoI_SH3)

(211-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

59.149

99.156

0.586