Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IMP99_RS02175 Genome accession   NZ_CP062855
Coordinates   468279..468956 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli O20:H12 strain Res13-Lact-PEB17-18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 463279..473956
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMP99_RS02160 (IMP99_02160) chiA 464646..467339 (+) 2694 WP_195686805.1 bifunctional chitinase/lysozyme -
  IMP99_RS02165 (IMP99_02165) bfd 467508..467702 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  IMP99_RS02170 (IMP99_02170) bfr 467774..468250 (+) 477 WP_000675504.1 bacterioferritin -
  IMP99_RS02175 (IMP99_02175) pilD 468279..468956 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  IMP99_RS02180 (IMP99_02180) gspM 468956..469417 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  IMP99_RS02185 (IMP99_02185) gspL 469414..470577 (-) 1164 WP_001115230.1 type II secretion system protein GspL -
  IMP99_RS02190 (IMP99_02190) gspK 470592..471575 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  IMP99_RS02195 (IMP99_02195) gspJ 471568..472155 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  IMP99_RS02200 (IMP99_02200) gspI 472148..472525 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  IMP99_RS02205 (IMP99_02205) gspH 472522..473031 (-) 510 WP_001076048.1 type II secretion system minor pseudopilin GspH -
  IMP99_RS02210 (IMP99_02210) gspG 473039..473476 (-) 438 WP_042032455.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=434853 IMP99_RS02175 WP_000178161.1 468279..468956(-) (pilD) [Escherichia coli O20:H12 strain Res13-Lact-PEB17-18]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=434853 IMP99_RS02175 WP_000178161.1 468279..468956(-) (pilD) [Escherichia coli O20:H12 strain Res13-Lact-PEB17-18]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378