Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IMQ02_RS01985 Genome accession   NZ_CP062853
Coordinates   432435..433112 (-) Length   225 a.a.
NCBI ID   WP_000178155.1    Uniprot ID   -
Organism   Escherichia coli O9:H10 strain Res13-Lact-PEB20-37-A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 427435..438112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMQ02_RS01970 (IMQ02_01970) chiA 428802..431495 (+) 2694 WP_195691163.1 bifunctional chitinase/lysozyme -
  IMQ02_RS01975 (IMQ02_01975) bfd 431664..431858 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  IMQ02_RS01980 (IMQ02_01980) bfr 431930..432406 (+) 477 WP_000675504.1 bacterioferritin -
  IMQ02_RS01985 (IMQ02_01985) pilD 432435..433112 (-) 678 WP_000178155.1 prepilin peptidase GspO Machinery gene
  IMQ02_RS01990 (IMQ02_01990) gspM 433112..433573 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  IMQ02_RS01995 (IMQ02_01995) gspL 433570..434733 (-) 1164 WP_001115235.1 type II secretion system protein GspL -
  IMQ02_RS02000 (IMQ02_02000) gspK 434748..435731 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  IMQ02_RS02005 (IMQ02_02005) gspJ 435724..436311 (-) 588 WP_000610636.1 type II secretion system minor pseudopilin GspJ -
  IMQ02_RS02010 (IMQ02_02010) gspI 436304..436681 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  IMQ02_RS02015 (IMQ02_02015) gspH 436678..437187 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  IMQ02_RS02020 (IMQ02_02020) gspG 437195..437632 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24879.74 Da        Isoelectric Point: 7.6578

>NTDB_id=434775 IMQ02_RS01985 WP_000178155.1 432435..433112(-) (pilD) [Escherichia coli O9:H10 strain Res13-Lact-PEB20-37-A]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFHQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=434775 IMQ02_RS01985 WP_000178155.1 432435..433112(-) (pilD) [Escherichia coli O9:H10 strain Res13-Lact-PEB20-37-A]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCACCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378