Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HC169_RS19735 Genome accession   NZ_CP050788
Coordinates   4215629..4217068 (-) Length   479 a.a.
NCBI ID   WP_139745550.1    Uniprot ID   -
Organism   Aeromonas media strain FiBL-4359     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4210629..4222068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HC169_RS19705 (HC169_19680) - 4210902..4211642 (+) 741 WP_404839587.1 2-dehydro-3-deoxy-phosphogluconate aldolase -
  HC169_RS19710 (HC169_19685) - 4211749..4213653 (+) 1905 WP_404839588.1 BglG family transcription antiterminator -
  HC169_RS19715 (HC169_19690) npr 4213672..4213944 (-) 273 WP_005331359.1 PTS phosphocarrier protein NPr -
  HC169_RS19720 (HC169_19695) rapZ 4213965..4214831 (-) 867 WP_289985687.1 RNase adapter RapZ -
  HC169_RS19725 (HC169_19700) ptsN 4214872..4215318 (-) 447 WP_289980653.1 PTS IIA-like nitrogen regulatory protein PtsN -
  HC169_RS19730 (HC169_19705) hpf 4215321..4215608 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  HC169_RS19735 (HC169_19710) rpoN/rpoN1 4215629..4217068 (-) 1440 WP_139745550.1 RNA polymerase factor sigma-54 Machinery gene
  HC169_RS19740 (HC169_19715) lptB 4217134..4217859 (-) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  HC169_RS19745 (HC169_19720) lptA 4217872..4218390 (-) 519 WP_025328531.1 lipopolysaccharide transport periplasmic protein LptA -
  HC169_RS19750 (HC169_19725) lptC 4218377..4218937 (-) 561 WP_042649050.1 LPS export ABC transporter periplasmic protein LptC -
  HC169_RS19755 (HC169_19730) kdsC 4218934..4219488 (-) 555 WP_106885625.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  HC169_RS19760 (HC169_19735) - 4219488..4220483 (-) 996 WP_404839589.1 KpsF/GutQ family sugar-phosphate isomerase -
  HC169_RS19765 (HC169_19740) mlaF 4220690..4221490 (+) 801 WP_025328535.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53792.76 Da        Isoelectric Point: 4.4177

>NTDB_id=434741 HC169_RS19735 WP_139745550.1 4215629..4217068(-) (rpoN/rpoN1) [Aeromonas media strain FiBL-4359]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAQETSPIEPQDASQLDSSDAMAQDKMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDESGYLTVSLED
IQAAVSSEEVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGTKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=434741 HC169_RS19735 WP_139745550.1 4215629..4217068(-) (rpoN/rpoN1) [Aeromonas media strain FiBL-4359]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAATAACCCCTTGCTGGAGCAGGAGGAGAATGAGG
CGCAGGAAACCAGCCCGATAGAGCCCCAGGACGCCAGTCAACTCGACTCCAGCGACGCCATGGCGCAGGACAAGATGCCG
GACGAACTGCCGGTGGACACCACCTGGGATGAGGTCTACTCCGCCGGTACGGCCCAGAGTGCCGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAAACTACCGAGAACCTGCAGGACTATCTGCTGTGGCAGATGCGCCTGACGCCGTTTA
GTGATCTGGATGCCGCCATCGCCCTCGCCATTATCGATGCCATCGATGAGTCCGGCTACCTGACCGTCTCTCTCGAAGAC
ATTCAGGCTGCAGTCAGCAGTGAAGAGGTAGAAGTTGAACTGGATGAAGTGGAAGCCGTGCTCAAGCGAGTGCAGCATTT
CGATCCGGTTGGCATAGCCGCGCGCTCGGTGCAGGAGTGTCTGCTGATCCAGCTTGGCCAGTACTCGCCGGATCTGCCCT
GGCTCAATGAGGCAAGGGAGGTGATCCGCGAGCACATGGATCTGCTCGGCAACCGCGACTACCGCACCCTGGCACGCAAG
ACCCGGCTCAAGGAGAGCGACCTCAAGGAGGTGCTGGATCTCATCCAGCAGCTGGAGCCGAGACCCGGCAATGGCATCAT
CCAGAGTGAATCGCAATACGTGATCCCGGACGTCTCCGTGGTCAAGAAGGGGACTAAGTGGGTGGTTGAGCTCAACCCGG
ATTCGGCGCCGCGCATCCGTGTCAACGAGGCCTATGCGGCCATGGCACGCAATGCCCGCAGCAGCACGGATACCCAGTTC
ATCCGCTCCCATCTGCAGGAAGCCAAATGGTTCATCAAGAGCCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCCAG
CTGCATAGTCGAGCAGCAGCAGGGCTTCTTCGAATACGGTGAAGAGGCCATGAAACCCATGGTACTCAACGACATCGCCG
AGGCGGTGGAGATGCACGAGTCGACGATTTCACGGGTGACGACCCAGAAGTACATGCATACGCCCCGTGGCATCTTTGAA
CTGAAGTTTTTTTTCTCCAGTCATGTCAACACGGAGGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCCCTGATCAA
GAAGCTGGTGACGGCCGAAAACCCGGCCAAACCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.471

100

0.495