Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   IMQ20_RS01950 Genome accession   NZ_CP062838
Coordinates   427904..428581 (-) Length   225 a.a.
NCBI ID   WP_100004177.1    Uniprot ID   -
Organism   Escherichia coli strain Res13-Lact-PER02-33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 422904..433581
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMQ20_RS01935 (IMQ20_01935) chiA 424271..426964 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  IMQ20_RS01940 (IMQ20_01940) bfd 427133..427327 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  IMQ20_RS01945 (IMQ20_01945) bfr 427399..427875 (+) 477 WP_000675504.1 bacterioferritin -
  IMQ20_RS01950 (IMQ20_01950) pilD 427904..428581 (-) 678 WP_100004177.1 prepilin peptidase GspO Machinery gene
  IMQ20_RS01955 (IMQ20_01955) gspM 428581..429042 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  IMQ20_RS01960 (IMQ20_01960) gspL 429039..430202 (-) 1164 WP_195686515.1 type II secretion system protein GspL -
  IMQ20_RS01965 (IMQ20_01965) gspK 430217..431200 (-) 984 WP_001058521.1 type II secretion system minor pseudopilin GspK -
  IMQ20_RS01970 (IMQ20_01970) gspJ 431193..431780 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  IMQ20_RS01975 (IMQ20_01975) gspI 431773..432150 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  IMQ20_RS01980 (IMQ20_01980) gspH 432147..432656 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  IMQ20_RS01985 (IMQ20_01985) gspG 432664..433101 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24926.84 Da        Isoelectric Point: 7.9563

>NTDB_id=434612 IMQ20_RS01950 WP_100004177.1 427904..428581(-) (pilD) [Escherichia coli strain Res13-Lact-PER02-33]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYVIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=434612 IMQ20_RS01950 WP_100004177.1 427904..428581(-) (pilD) [Escherichia coli strain Res13-Lact-PER02-33]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGTCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378