Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   HCU65_RS06315 Genome accession   NZ_CP050705
Coordinates   1268369..1268794 (+) Length   141 a.a.
NCBI ID   WP_219947784.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain PENSV20     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1263369..1273794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HCU65_RS06290 (HCU65_06300) - 1263816..1265465 (+) 1650 WP_258235479.1 right-handed parallel beta-helix repeat-containing protein -
  HCU65_RS06295 (HCU65_06305) - 1265477..1265764 (+) 288 WP_228370114.1 hypothetical protein -
  HCU65_RS20885 - 1266094..1266243 (-) 150 WP_309143184.1 FCD domain-containing protein -
  HCU65_RS06305 (HCU65_06320) - 1267511..1267840 (-) 330 WP_010788288.1 hypothetical protein -
  HCU65_RS06310 (HCU65_06325) - 1267900..1268178 (-) 279 WP_061670553.1 hypothetical protein -
  HCU65_RS06315 (HCU65_06330) nucA/comI 1268369..1268794 (+) 426 WP_219947784.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  HCU65_RS06320 (HCU65_06335) - 1268943..1269605 (+) 663 WP_010788290.1 MgtC/SapB family protein -
  HCU65_RS06325 (HCU65_06340) - 1269664..1269903 (-) 240 WP_003327512.1 hypothetical protein -
  HCU65_RS06330 (HCU65_06345) - 1270116..1270328 (-) 213 WP_003327511.1 aspartyl-phosphate phosphatase Spo0E family protein -
  HCU65_RS06335 (HCU65_06350) - 1270388..1270603 (-) 216 WP_072053284.1 spore coat protein -
  HCU65_RS06340 (HCU65_06355) - 1270917..1272317 (+) 1401 WP_219947385.1 polysaccharide deacetylase family protein -
  HCU65_RS06345 (HCU65_06360) - 1272370..1272843 (-) 474 WP_061670904.1 YjfA family protein -
  HCU65_RS06350 (HCU65_06365) - 1272979..1273389 (-) 411 WP_219947386.1 DUF2809 domain-containing protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15533.39 Da        Isoelectric Point: 4.9399

>NTDB_id=434111 HCU65_RS06315 WP_219947784.1 1268369..1268794(+) (nucA/comI) [Bacillus atrophaeus strain PENSV20]
MKKWITSLFLTAAILFGLMGAEVYAPQDVKGASRYDQVLYFPFSRYPETGDHIKDAIAAGHSDVCTIDRAGADERREESL
KGVPTKRGYDRDEWPMAVCEEGGTGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 426 bp        

>NTDB_id=434111 HCU65_RS06315 WP_219947784.1 1268369..1268794(+) (nucA/comI) [Bacillus atrophaeus strain PENSV20]
ATGAAAAAATGGATCACGAGCTTATTTCTGACCGCAGCGATATTGTTTGGATTGATGGGTGCGGAAGTGTATGCTCCGCA
GGATGTCAAAGGCGCATCACGGTATGATCAGGTTCTTTACTTCCCATTTTCACGTTATCCTGAAACCGGCGATCACATTA
AAGATGCGATAGCGGCCGGGCATTCAGATGTATGTACGATTGACAGGGCCGGGGCGGATGAGCGCAGGGAGGAGTCTTTA
AAGGGGGTTCCTACGAAACGGGGGTATGACCGGGATGAGTGGCCGATGGCTGTATGTGAAGAAGGCGGGACCGGGGCGGA
TATCCGGTATGTCACCCCATCTGATAATCGAGGCGCCGGTTCATGGGTCGGGAATCAAATGAGCGGTTATTCTGATGGTA
CGAGAGTATTATTTATCGTACAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

60.317

89.362

0.539