Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HCU70_RS03285 Genome accession   NZ_CP050690
Coordinates   672125..673489 (-) Length   454 a.a.
NCBI ID   WP_001085213.1    Uniprot ID   -
Organism   Staphylococcus aureus strain PMB179-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 667125..678489
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HCU70_RS03270 (HCU70_03270) cysE 667946..668593 (-) 648 WP_001805237.1 serine O-acetyltransferase -
  HCU70_RS03275 (HCU70_03275) gltX 669016..670470 (-) 1455 WP_001283797.1 glutamate--tRNA ligase -
  HCU70_RS03280 (HCU70_03280) - 671027..672100 (-) 1074 WP_001028376.1 PIN/TRAM domain-containing protein -
  HCU70_RS03285 (HCU70_03285) radA 672125..673489 (-) 1365 WP_001085213.1 DNA repair protein RadA Machinery gene
  HCU70_RS03290 (HCU70_03290) - 673974..676430 (-) 2457 WP_000897132.1 ATP-dependent Clp protease ATP-binding subunit -
  HCU70_RS03295 (HCU70_03295) - 676444..677451 (-) 1008 WP_000149503.1 protein arginine kinase -
  HCU70_RS03300 (HCU70_03300) - 677441..678007 (-) 567 WP_000882069.1 UvrB/UvrC motif-containing protein -
  HCU70_RS03305 (HCU70_03305) - 678026..678487 (-) 462 WP_000551762.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49849.38 Da        Isoelectric Point: 7.0049

>NTDB_id=433937 HCU70_RS03285 WP_001085213.1 672125..673489(-) (radA) [Staphylococcus aureus strain PMB179-1]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIIEKAANPKHGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSFALHS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=433937 HCU70_RS03285 WP_001085213.1 672125..673489(-) (radA) [Staphylococcus aureus strain PMB179-1]
TTGGCCAAGAAAAAAGTGATTTTTGAATGTATGGCTTGTGGTTATCAATCTCCTAAATGGATGGGGAAATGTCCTAATTG
TGGCGCTTGGAATCAAATGGAGGAAATTATTGAAAAAGCAGCCAATCCTAAACATGGAGTTAAAACCAAGGAATTAGCAG
GTAAAGTACAAAAATTAAATAGTATTAAACATGAAACAACGCCGAGAGTGTTAACAGATTCAGCAGAATTCAACCGTGTA
TTAGGTGGAGGTATTGTGAGCGGATCGTTAGTACTTATTGGTGGGGATCCAGGTATTGGTAAGTCAACGTTACTTTTACA
AATTTGTGCATCGTTATCTCAAAAGAAAAAAGTACTATATATTACTGGAGAAGAATCGCTTAGTCAGACTAAATTACGTG
CAGAGCGATTAGATGAAGATTCAAGTGAATTGCAAGTATTAGCTGAAACAGATCTTGAAGTTATTTATCAAACAGTAAAA
GAAGAACAACCTGATTTATTAGTAGTGGATTCGATTCAAACAATATATCATCCTGAAATCAGCTCTGCGCCAGGTTCTGT
TTCACAAGTTCGTGAAAGTACACAAAGTTTAATGAATATTGCTAAACAAATGAACATTGCAACTTTTATAGTGGGTCATG
TAACGAAAGAAGGTCAAATTGCTGGCCCAAGATTGCTAGAACACATGGTTGATACTGTGCTTTATTTTGAAGGCGATGAA
CACCACGCATATCGAATTTTGCGAGCTGTTAAAAACCGTTTTGGTTCAACGAATGAAATGGGAATCTTCGAAATGAAGCA
AAGTGGATTAAAAGGTGTAAATAATCCATCTGAAATGTTTTTAGAAGAACGTTCAACAAATGTTCCAGGTTCAACAATTG
TTGCAACCATGGAGGGAACCAGACCACTTTTAATAGAAGTTCAAGCGCTGGTAACTCCAACGACTTTTAACAATCCGAGA
CGAATGGCAACAGGGATTGATCATAATCGATTAAGTTTGTTGATGGCTGTTTTGGAAAAGAAAGAAAATTATCTATTACA
ACAACAAGATGCTTATATCAAAGTAGCTGGCGGTGTAAAGTTAACGGAGCCAGCAGTTGATTTAAGTGTAATTGTAGCAA
CTGCATCTAGCTTTAAAGATAAAGCTGTCGACGGATTAGATTGCTATATTGGAGAAGTTGGTTTAACGGGTGAGGTACGT
CGTGTATCTCGGATAGAACAACGCGTGCAAGAGGCTGCAAAACTAGGTTTCAAACGTGTAATTATTCCTAAAAATAATAT
AGGCGGATGGACATATCCTGAAGGTATACAAGTAATAGGTGTAACTACTGTACATGAAGCATTGTCATTTGCTCTTCATT
CATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(77-218)

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.885

100

0.676
  radA Streptococcus pneumoniae Rx1

61.83

98.678

0.61
  radA Streptococcus pneumoniae D39

61.83

98.678

0.61
  radA Streptococcus pneumoniae R6

61.83

98.678

0.61
  radA Streptococcus pneumoniae TIGR4

61.83

98.678

0.61
  radA Streptococcus mitis NCTC 12261

61.83

98.678

0.61
  radA Streptococcus mitis SK321

61.83

98.678

0.61