Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HC354_RS00990 Genome accession   NZ_CP050650
Coordinates   204238..205608 (-) Length   456 a.a.
NCBI ID   WP_002293096.1    Uniprot ID   A0A4Y3JR63
Organism   Enterococcus faecium strain BIOPOP-3 WT     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 199238..210608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HC354_RS00975 (HC354_00975) epsC 200561..201097 (-) 537 WP_002293094.1 serine O-acetyltransferase EpsC -
  HC354_RS00980 (HC354_00980) gltX 201417..202877 (-) 1461 WP_167778985.1 glutamate--tRNA ligase -
  HC354_RS00985 (HC354_00985) - 203047..204204 (-) 1158 WP_002287448.1 PIN/TRAM domain-containing protein -
  HC354_RS00990 (HC354_00990) radA 204238..205608 (-) 1371 WP_002293096.1 DNA repair protein RadA Machinery gene
  HC354_RS00995 (HC354_00995) - 205671..206198 (-) 528 WP_002345653.1 dUTP diphosphatase -
  HC354_RS01000 (HC354_01000) proB 206428..207234 (+) 807 WP_002344833.1 glutamate 5-kinase -
  HC354_RS01005 (HC354_01005) - 207231..208478 (+) 1248 WP_002310461.1 glutamate-5-semialdehyde dehydrogenase -
  HC354_RS01010 (HC354_01010) - 208554..208898 (+) 345 WP_002310463.1 hypothetical protein -
  HC354_RS01015 (HC354_01015) - 209015..209341 (-) 327 WP_002310464.1 hypothetical protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49935.60 Da        Isoelectric Point: 7.7416

>NTDB_id=433878 HC354_RS00990 WP_002293096.1 204238..205608(-) (radA) [Enterococcus faecium strain BIOPOP-3 WT]
MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTDRRTRTSLTGEKAKPTKIADVVPKKEPRVKTKLEELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEVANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECFIGEIGLTG
EIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAETLKRVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=433878 HC354_RS00990 WP_002293096.1 204238..205608(-) (radA) [Enterococcus faecium strain BIOPOP-3 WT]
ATGGCAAAAAAATCAAAAGTACAATTTGTTTGCCAAAATTGCGGGTATGTGTCGCCTAAGTTCTTGGGACGCTGCCCAAA
TTGCGGAAAATGGAACACGATGGTCGAAGAAATCGAACAAGATACAACAGACCGCAGAACGCGGACAAGTTTGACAGGAG
AAAAAGCGAAACCAACAAAAATCGCAGACGTCGTCCCTAAAAAAGAACCTCGGGTCAAAACGAAATTAGAAGAACTGAAC
CGTGTGCTAGGTGGAGGCGTGGTTCCGGGATCCATGGTCTTGATTGGTGGAGATCCAGGTATTGGTAAATCCACTTTGCT
GTTACAAGTTTCTCAACAATTAGCAGCCATAGGCGGGAAAGTCTTGTATGTATCCGGCGAAGAAAGCGCAGAACAGATAA
AATTACGCGCGGAGCGCCTCGGAAGTATCAACACAGAATTTTATCTATACGCTGAAACAGATATGAATGAAATCAGTCGT
GCCATCGAACATATCTCCCCTGATTATGTTATTATTGATTCCATCCAAACGATGACGCAGCCAGACATCACAAGTGTGGC
AGGAAGCGTCAGCCAAGTAAGAGAAACCACTGCAGAATTACTCAAAATCGCTAAAACGAATGGAATTGCCATTTTTATCG
TCGGTCATGTTACGAAAGAAGGTTCGATCGCCGGTCCAAGAATGTTAGAGCATATGGTGGATACAGTGTTATATTTTGAA
GGTGAACAACATCATAGTTTCCGAATTTTACGAGCGGTAAAAAACCGATTTGGCTCAACGAACGAGATTGGGATTTTTGA
AATGCATGAGCATGGATTGGAAGAAGTCGCTAACCCTTCACAGATTTTCTTGGAGGAACGCCTAGACGGTGCGACTGGCT
CTGCTATCGTTGTAGCAATGGAAGGAACACGCCCAATTTTGGTAGAGATCCAAGCATTAGTCACACCTACTATGTTCGGA
AATGCTAAGCGAACAACAACAGGTCTTGATTTCAATCGTGTATCTCTAATCATGGCAGTGCTGGAAAAAAGAGCTGGACT
ACTTCTTCAGAATCAAGATGCTTACTTAAAAGCTGCAGGTGGGGTAAAATTAAATGAACCAGCGATTGATTTAGCTATCG
CTGTCAGTATTGCTTCAAGCTACAAAGAAAAAGGAACACAGCCGACGGAATGTTTCATCGGTGAAATCGGACTGACTGGA
GAAATCAGACGTGTCAGTCATATTGAACAACGGGTCAAAGAAGTCCAAAAACTCGGCTTCACAAAAGTTTACCTGCCTAA
AAACAACTTAGGCAACTGGGAAGCGCCAAAAGGAATCGAGATCGTTGGAGTAGCAACTTTGGCTGAAACGTTGAAGCGTG
TGTTCCGCTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

AAA domain (AAA_25)

(70-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y3JR63

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.286

99.781

0.741
  radA Streptococcus pneumoniae Rx1

74.286

99.781

0.741
  radA Streptococcus pneumoniae D39

74.286

99.781

0.741
  radA Streptococcus pneumoniae R6

74.286

99.781

0.741
  radA Streptococcus pneumoniae TIGR4

74.286

99.781

0.741
  radA Streptococcus mitis SK321

74.066

99.781

0.739
  radA Bacillus subtilis subsp. subtilis str. 168

67.627

98.904

0.669