Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HB664_RS00725 Genome accession   NZ_CP050520
Coordinates   132364..133797 (+) Length   477 a.a.
NCBI ID   WP_032659700.1    Uniprot ID   -
Organism   Enterobacter sp. DNB-S2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 127364..138797
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB664_RS00695 (HB664_00700) - 127931..128908 (+) 978 WP_182382750.1 calcium/sodium antiporter -
  HB664_RS00700 (HB664_00705) kdsD 128922..129908 (+) 987 WP_032659705.1 arabinose-5-phosphate isomerase KdsD -
  HB664_RS00705 (HB664_00710) kdsC 129923..130489 (+) 567 WP_023309356.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  HB664_RS00710 (HB664_00715) lptC 130486..131061 (+) 576 WP_033146749.1 LPS export ABC transporter periplasmic protein LptC -
  HB664_RS00715 (HB664_00720) lptA 131030..131584 (+) 555 WP_033146750.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  HB664_RS00720 (HB664_00725) lptB 131591..132316 (+) 726 WP_033146751.1 LPS export ABC transporter ATP-binding protein -
  HB664_RS00725 (HB664_00730) rpoN/rpoN1 132364..133797 (+) 1434 WP_032659700.1 RNA polymerase factor sigma-54 Machinery gene
  HB664_RS00730 (HB664_00735) hpf 133820..134107 (+) 288 WP_008501444.1 ribosome hibernation promoting factor -
  HB664_RS00735 (HB664_00740) ptsN 134190..134681 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  HB664_RS00740 (HB664_00745) rapZ 134727..135581 (+) 855 WP_014885397.1 RNase adapter RapZ -
  HB664_RS00745 (HB664_00750) npr 135578..135850 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  HB664_RS00750 (HB664_00755) mtgA 135890..136615 (-) 726 WP_219940269.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  HB664_RS00755 (HB664_00760) elbB 136612..137265 (-) 654 WP_033146753.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54169.89 Da        Isoelectric Point: 4.3149

>NTDB_id=433580 HB664_RS00725 WP_032659700.1 132364..133797(+) (rpoN/rpoN1) [Enterobacter sp. DNB-S2]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=433580 HB664_RS00725 WP_032659700.1 132364..133797(+) (rpoN/rpoN1) [Enterobacter sp. DNB-S2]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCAATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACTCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGAGAGACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTCACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATCCTGGAAAGCATGGGCGACGACGAGATTGAGCTTGAGGAAGTCGAAGCCGTTCTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGCTCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGTTGGCCAACCACGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCACTCGATCCGCGCCCGGGACAGTCGATCCAGACCAG
CGAGCCCGAGTACGTCATCCCGGACGTGCTGGTGAGAAAACACAATGACCGCTGGGTCGTTGAACTGAATTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAAAGCCGAAATGATACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATATTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48