Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HCU35_RS02400 Genome accession   NZ_CP050506
Coordinates   509161..510594 (-) Length   477 a.a.
NCBI ID   WP_003861864.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain CM18-242-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 504161..515594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HCU35_RS02370 (HCU35_02375) elbB 505693..506346 (+) 654 WP_015571987.1 isoprenoid biosynthesis glyoxalase ElbB -
  HCU35_RS02375 (HCU35_02380) mtgA 506343..507068 (+) 726 WP_017382728.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  HCU35_RS02380 (HCU35_02385) npr 507108..507380 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  HCU35_RS02385 (HCU35_02390) rapZ 507377..508231 (-) 855 WP_003861870.1 RNase adapter RapZ -
  HCU35_RS02390 (HCU35_02395) ptsN 508277..508768 (-) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  HCU35_RS02395 (HCU35_02400) hpf 508851..509138 (-) 288 WP_003861866.1 ribosome hibernation promoting factor -
  HCU35_RS02400 (HCU35_02405) rpoN/rpoN1 509161..510594 (-) 1434 WP_003861864.1 RNA polymerase factor sigma-54 Machinery gene
  HCU35_RS02405 (HCU35_02410) lptB 510642..511367 (-) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  HCU35_RS02410 (HCU35_02415) lptA 511374..511928 (-) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  HCU35_RS02415 (HCU35_02420) lptC 511897..512472 (-) 576 WP_015571983.1 LPS export ABC transporter periplasmic protein LptC -
  HCU35_RS02420 (HCU35_02425) kdsC 512469..513035 (-) 567 WP_017382729.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  HCU35_RS02425 (HCU35_02430) kdsD 513050..514036 (-) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  HCU35_RS02430 (HCU35_02435) - 514050..515027 (-) 978 WP_003861852.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54099.96 Da        Isoelectric Point: 4.3358

>NTDB_id=433487 HCU35_RS02400 WP_003861864.1 509161..510594(-) (rpoN/rpoN1) [Enterobacter hormaechei strain CM18-242-2]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=433487 HCU35_RS02400 WP_003861864.1 509161..510594(-) (rpoN/rpoN1) [Enterobacter hormaechei strain CM18-242-2]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTTGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGACGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGCGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACCCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTGGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTTCTGAAACGCGTTCAGCGTTTCGACCC
GATAGGTGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAATCATGACTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
CGAACCGGAATATGTCATTCCTGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCACGTAATGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGCCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAATATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48