Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HCJ88_RS10470 Genome accession   NZ_CP050500
Coordinates   2137579..2138943 (-) Length   454 a.a.
NCBI ID   WP_003567254.1    Uniprot ID   A0A829GUQ3
Organism   Lacticaseibacillus paracasei strain NFFJ04     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2132579..2143943
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HCJ88_RS10445 (HCJ88_10635) - 2133403..2134179 (-) 777 WP_003591638.1 ABC transporter permease subunit -
  HCJ88_RS10450 (HCJ88_10640) - 2134197..2134574 (-) 378 Protein_2051 ABC transporter ATP-binding protein -
  HCJ88_RS10455 (HCJ88_10645) - 2134686..2135606 (+) 921 WP_003574021.1 IS30 family transposase -
  HCJ88_RS10460 (HCJ88_10650) - 2135627..2136139 (-) 513 Protein_2053 ATP-binding cassette domain-containing protein -
  HCJ88_RS10465 (HCJ88_10655) - 2136442..2137557 (-) 1116 WP_193137136.1 PIN domain-containing protein -
  HCJ88_RS10470 (HCJ88_10660) radA 2137579..2138943 (-) 1365 WP_003567254.1 DNA repair protein RadA Machinery gene
  HCJ88_RS10475 (HCJ88_10665) - 2138960..2139502 (-) 543 WP_003567256.1 dUTP diphosphatase -
  HCJ88_RS10480 (HCJ88_10670) - 2139723..2140013 (+) 291 WP_003567258.1 GNAT family N-acetyltransferase -
  HCJ88_RS10485 (HCJ88_10675) - 2140129..2140506 (-) 378 WP_003567260.1 DUF805 domain-containing protein -
  HCJ88_RS10490 (HCJ88_10680) - 2140751..2142070 (+) 1320 WP_003571381.1 C1 family peptidase -
  HCJ88_RS10495 (HCJ88_10685) - 2142393..2143739 (+) 1347 WP_003576225.1 C1 family peptidase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49126.47 Da        Isoelectric Point: 7.4216

>NTDB_id=433419 HCJ88_RS10470 WP_003567254.1 2137579..2138943(-) (radA) [Lacticaseibacillus paracasei strain NFFJ04]
MAKAKTQYVCQNCGYISATYLGRCPNCGGWNTLVEETVSSSKSVPRQTAAGSKVKPTRMNDVTITKETRVKTGLDELNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSGQLANTGGTVLYVSGEESASQIKMRAGRLGVANSGMYLYPETDMPSIEDVI
NQMQPDYVVIDSVQTMNVPEMNSAVGSVAQIREVTAELMRIAKSKGVTIFIVGHVTKEGAIAGPKILEHMVDTVLYFEGD
MHHTYRILRSVKNRFGSTNEIGIFEMHQDGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVEIQALISPTMYGNA
KRTSSGLDHNRVSLIMAVLEKRANLMLQNQDAYLKATGGVKLDEPAIDLAMAVAIASSYRDKEISPTDCFVGEIGLTGEV
RRVNRIEERVKEAAKLGFKRIFVPRNNLQGWHAPKDIQVIGVTSIAEALHKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=433419 HCJ88_RS10470 WP_003567254.1 2137579..2138943(-) (radA) [Lacticaseibacillus paracasei strain NFFJ04]
ATGGCGAAAGCAAAAACACAATATGTTTGCCAAAACTGTGGCTATATTTCAGCCACTTATCTAGGCCGGTGTCCAAATTG
CGGCGGCTGGAACACCTTAGTGGAAGAAACCGTCAGTTCAAGCAAGTCTGTGCCGCGACAAACAGCAGCCGGCAGTAAGG
TAAAGCCGACGCGGATGAACGATGTGACGATCACCAAAGAAACGCGCGTCAAGACTGGCTTAGATGAACTGAACCGGGTG
CTTGGCGGCGGAGTGGTGCCAGGGTCACTGGTGCTGATCGGCGGGGATCCGGGGATTGGTAAATCAACCTTGCTATTACA
AGTATCAGGGCAGTTGGCTAATACCGGCGGAACCGTTTTGTATGTCTCTGGCGAAGAAAGTGCCAGTCAGATCAAAATGC
GGGCTGGCCGGCTTGGTGTGGCAAATTCGGGGATGTACTTGTATCCCGAAACCGACATGCCGAGTATTGAAGACGTCATC
AACCAAATGCAGCCGGATTACGTTGTGATCGACTCCGTTCAAACTATGAATGTGCCAGAAATGAATTCAGCGGTCGGTTC
TGTGGCACAGATTCGTGAAGTGACGGCAGAACTGATGCGCATTGCCAAGTCCAAAGGCGTGACCATTTTCATCGTTGGCC
ATGTCACTAAGGAAGGGGCGATTGCCGGGCCGAAGATTCTTGAGCATATGGTCGACACGGTGCTCTATTTTGAAGGCGAC
ATGCACCACACGTATCGAATCTTAAGATCCGTCAAAAATCGATTTGGATCGACCAATGAAATCGGCATTTTTGAAATGCA
TCAAGACGGGTTACAGGAAGTCGCCAATCCCTCGGAAATCTTCTTGGAAGAACGCTTGGCGGGGGCAACCGGTTCTGCGG
TGGTTGTTTCGATGGAAGGCACCCGGCCAATCTTAGTCGAGATTCAAGCCTTGATCAGTCCAACGATGTATGGCAATGCT
AAACGGACGAGCAGTGGGTTGGATCACAATCGAGTGAGCTTGATCATGGCCGTTCTTGAGAAGCGGGCTAACTTGATGTT
GCAAAATCAGGACGCCTATCTCAAGGCAACAGGCGGTGTGAAACTCGATGAGCCGGCGATTGACCTCGCCATGGCTGTGG
CAATTGCGTCATCTTATCGAGATAAGGAGATTTCACCGACAGATTGTTTTGTCGGTGAAATCGGATTGACGGGGGAAGTG
CGCCGCGTCAATCGGATCGAAGAACGTGTCAAAGAGGCGGCCAAACTGGGTTTCAAACGGATTTTTGTACCACGTAACAA
CTTGCAAGGCTGGCATGCGCCAAAAGATATTCAAGTCATTGGTGTTACAAGCATTGCCGAAGCGCTGCATAAGGTCTTTA
ATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(69-219)

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A829GUQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

68.212

99.78

0.681
  radA Streptococcus pneumoniae Rx1

68.212

99.78

0.681
  radA Streptococcus pneumoniae D39

68.212

99.78

0.681
  radA Streptococcus pneumoniae R6

68.212

99.78

0.681
  radA Streptococcus pneumoniae TIGR4

68.212

99.78

0.681
  radA Streptococcus mitis SK321

67.991

99.78

0.678
  radA Bacillus subtilis subsp. subtilis str. 168

64.758

100

0.648