Detailed information    

insolico Bioinformatically predicted

Overview

Name   recN   Type   Machinery gene
Locus tag   EGM182_RS07255 Genome accession   NZ_CP050490
Coordinates   1369284..1370957 (+) Length   557 a.a.
NCBI ID   WP_016610293.1    Uniprot ID   A0A377KTG8
Organism   Enterococcus casseliflavus strain EGM182     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1364284..1375957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGM182_RS07225 (EGM182_07325) - 1364604..1365455 (+) 852 WP_086292168.1 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase -
  EGM182_RS07230 (EGM182_07330) xseA 1365456..1366808 (+) 1353 WP_010747995.1 exodeoxyribonuclease VII large subunit -
  EGM182_RS07235 (EGM182_07335) - 1366789..1367010 (+) 222 WP_005225774.1 exodeoxyribonuclease VII small subunit -
  EGM182_RS07240 (EGM182_07340) - 1367007..1367903 (+) 897 WP_118214936.1 polyprenyl synthetase family protein -
  EGM182_RS07245 (EGM182_07345) - 1367900..1368721 (+) 822 WP_118214935.1 TlyA family RNA methyltransferase -
  EGM182_RS07250 (EGM182_07350) argR 1368819..1369268 (+) 450 WP_005225777.1 arginine repressor -
  EGM182_RS07255 (EGM182_07355) recN 1369284..1370957 (+) 1674 WP_016610293.1 DNA repair protein RecN Machinery gene
  EGM182_RS07260 (EGM182_07360) - 1371053..1371784 (+) 732 WP_182454454.1 alpha/beta hydrolase -
  EGM182_RS07265 (EGM182_07365) - 1372186..1373040 (+) 855 WP_010748000.1 phosphate ABC transporter substrate-binding protein PstS -
  EGM182_RS07270 (EGM182_07370) pstC 1373138..1374058 (+) 921 WP_005225781.1 phosphate ABC transporter permease subunit PstC -
  EGM182_RS07275 (EGM182_07375) pstA 1374058..1374942 (+) 885 WP_005225782.1 phosphate ABC transporter permease PstA -
  EGM182_RS07280 (EGM182_07380) pstB 1374956..1375762 (+) 807 WP_005225783.1 phosphate ABC transporter ATP-binding protein PstB -

Sequence

Protein


Download         Length: 557 a.a.        Molecular weight: 62575.69 Da        Isoelectric Point: 4.5175

>NTDB_id=433286 EGM182_RS07255 WP_016610293.1 1369284..1370957(+) (recN) [Enterococcus casseliflavus strain EGM182]
MLLELSIQNFAIISNLHLSFHEGMTALTGETGAGKSIIIDAMGLLAGGRGSSDYLRQGAEKCRLEGIFEWPNQQEFKELT
ADLGIDEEEVLIVQRDISQSGKTICRVNGRTVTLSVLRQIGLFLVDIQGQNEHQELLQPEKHLALMDGFGDDAFKAELAS
YRQAYQAYRSLEKHVRTIQENEQLYVQRMDMLRFQQEEIAQAELMENEEEQLIDEREKLTNYQKIVDALGQSYGALSAEE
VNSLDGVSVALSEIQSIAHLDPAYEKISEAIQSAYYLLQDAATDISRQVDNLELDEGRLEEVISRLETIRQLKRKYGESI
PVILAYYDEISKEISESAYTEGQLEQLEEELAQKAEAVWQLAEALHVKRRELAHRLEKEIVQELKELYMEHAQFEVRFAS
GSKQLSSSGFDTVEFYLTTNPGEGMKPLVRVASGGELSRILLALKSIFSRKQGITSIVFDEVDTGVSGRVAQAIAEKIAK
IAANSQVLCITHLPQVAAVADNQYFIEKAIQEGRTETHVRKLSEEERVAEIARMLSGAEITDLTIEHARELLALAHR

Nucleotide


Download         Length: 1674 bp        

>NTDB_id=433286 EGM182_RS07255 WP_016610293.1 1369284..1370957(+) (recN) [Enterococcus casseliflavus strain EGM182]
ATGCTTTTAGAATTGTCGATCCAAAATTTTGCGATTATCTCCAACCTGCACCTGTCTTTTCATGAGGGGATGACGGCTCT
AACAGGTGAAACAGGTGCTGGGAAATCAATCATTATCGATGCGATGGGCTTACTAGCAGGTGGCAGAGGATCCAGTGACT
ATTTGCGTCAAGGCGCAGAAAAATGCCGTTTGGAAGGGATTTTTGAATGGCCGAATCAGCAGGAGTTCAAAGAGCTGACC
GCAGATCTGGGTATTGATGAAGAGGAAGTTCTGATCGTACAACGGGATATCTCTCAATCGGGTAAAACTATTTGTCGTGT
TAATGGTCGAACCGTTACCTTGTCGGTTTTGCGCCAAATCGGCTTGTTTTTAGTTGATATCCAAGGACAAAATGAGCATC
AAGAACTGCTGCAGCCAGAAAAACATTTGGCGTTGATGGATGGTTTTGGAGATGATGCCTTTAAAGCGGAACTTGCAAGT
TACCGTCAAGCCTATCAAGCCTATCGCTCCTTAGAAAAACATGTGCGGACCATTCAAGAAAATGAACAGCTCTATGTGCA
GCGAATGGACATGCTGCGTTTTCAACAAGAAGAGATCGCCCAAGCGGAATTGATGGAAAATGAAGAAGAGCAGCTGATCG
ATGAACGAGAAAAACTGACCAATTATCAGAAAATCGTGGATGCTCTAGGGCAAAGCTATGGCGCTCTATCCGCCGAAGAA
GTTAATAGCCTAGATGGAGTCAGTGTGGCATTATCAGAGATCCAGTCGATCGCCCATCTTGATCCTGCGTATGAAAAAAT
CAGTGAAGCGATCCAAAGTGCGTATTACTTGTTGCAAGATGCAGCCACTGATATTTCGCGGCAAGTCGACAACCTTGAAT
TAGATGAAGGCCGACTGGAAGAAGTCATTTCACGACTAGAAACGATTCGTCAATTGAAGCGCAAATACGGGGAGTCAATT
CCTGTGATCCTTGCTTATTATGACGAGATCAGCAAAGAAATCAGCGAATCAGCCTATACGGAAGGTCAGTTGGAGCAATT
AGAAGAAGAGCTTGCCCAAAAAGCTGAAGCAGTTTGGCAGCTGGCAGAAGCCTTGCACGTCAAACGACGTGAACTGGCTC
ATCGTTTAGAAAAAGAAATCGTTCAAGAGTTAAAAGAGCTGTATATGGAGCACGCGCAATTTGAAGTACGGTTTGCATCT
GGCTCAAAACAATTAAGCAGCAGCGGCTTTGATACCGTGGAGTTTTATTTGACCACCAACCCCGGTGAAGGCATGAAACC
GTTAGTGCGAGTTGCTTCTGGAGGAGAGCTTTCCCGGATCTTATTAGCGCTTAAATCGATTTTCTCCCGCAAGCAAGGAA
TCACTAGTATTGTCTTTGATGAGGTGGATACTGGGGTCAGTGGCCGAGTAGCTCAGGCGATCGCCGAAAAAATCGCCAAG
ATCGCTGCGAATTCGCAAGTCTTGTGTATCACCCACTTGCCGCAAGTAGCGGCTGTGGCGGACAATCAATATTTTATCGA
AAAAGCAATTCAAGAAGGTCGGACAGAAACCCATGTCCGTAAATTATCAGAAGAAGAACGAGTGGCTGAGATCGCCCGTA
TGCTCTCAGGTGCAGAAATCACCGATTTAACAATCGAACATGCACGAGAACTATTGGCGCTGGCTCACCGATAA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-508)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A377KTG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recN Bacillus subtilis subsp. subtilis str. 168

47.719

100

0.488