Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EGM182_RS00880 Genome accession   NZ_CP050490
Coordinates   22842..24212 (+) Length   456 a.a.
NCBI ID   WP_005226394.1    Uniprot ID   A0A242CQN0
Organism   Enterococcus casseliflavus strain EGM182     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 17842..29212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGM182_RS00850 (EGM182_00860) - 18309..19127 (+) 819 WP_005232914.1 ZIP family metal transporter -
  EGM182_RS00855 (EGM182_00865) - 19183..19386 (-) 204 WP_010748476.1 hypothetical protein -
  EGM182_RS00860 (EGM182_00870) - 19537..19938 (+) 402 WP_010748477.1 DUF4809 family protein -
  EGM182_RS00865 (EGM182_00875) - 20042..21286 (-) 1245 WP_192415816.1 glutamate-5-semialdehyde dehydrogenase -
  EGM182_RS00870 (EGM182_00880) proB 21283..22098 (-) 816 WP_060791699.1 glutamate 5-kinase -
  EGM182_RS00875 (EGM182_00885) - 22288..22797 (+) 510 WP_010748479.1 hypothetical protein -
  EGM182_RS00880 (EGM182_00890) radA 22842..24212 (+) 1371 WP_005226394.1 DNA repair protein RadA Machinery gene
  EGM182_RS00885 (EGM182_00895) - 24381..25520 (+) 1140 WP_005226396.1 PIN/TRAM domain-containing protein -
  EGM182_RS00890 (EGM182_00900) gltX 25690..27147 (+) 1458 WP_016608945.1 glutamate--tRNA ligase -
  EGM182_RS00895 (EGM182_00905) epsC 27407..27943 (+) 537 WP_118215703.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49604.28 Da        Isoelectric Point: 8.3034

>NTDB_id=433277 EGM182_RS00880 WP_005226394.1 22842..24212(+) (radA) [Enterococcus casseliflavus strain EGM182]
MAKKAKSQFVCQNCGYSSPKYLGKCPNCGSWNSMVEEVIQETADRRVRTTLTGQKMQPTKIADVVPKKEPRVQTKLAELN
RVLGGGVVPGSLILIGGDPGIGKSTLLLQVSQQLAAIGGTVLYVSGEESAEQIKMRAQRLGAIDTEFYLYGETDMSEISK
AIENIRPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEKHHSFRILRAVKNRFGSTNEIGIFEMEEGGLKEVANPSQVFLEERLEGATGSAIVVAMEGSRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTSPTECFIGEIGLTG
EIRRVNAIEQRVKEVQKLGFTKVYLPKNNLTGWQPPKGIEVEGVSTLAETLRKVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=433277 EGM182_RS00880 WP_005226394.1 22842..24212(+) (radA) [Enterococcus casseliflavus strain EGM182]
ATGGCAAAAAAAGCAAAAAGTCAATTTGTCTGTCAAAATTGTGGGTATAGTTCCCCTAAATATTTAGGGAAATGCCCCAA
TTGCGGCAGCTGGAATTCAATGGTGGAAGAAGTGATCCAAGAAACCGCCGATCGTCGAGTACGTACGACATTGACGGGGC
AAAAGATGCAGCCGACGAAAATCGCCGATGTCGTACCGAAAAAAGAACCGCGGGTCCAAACGAAACTCGCTGAGTTGAAT
CGCGTCTTAGGCGGCGGCGTGGTCCCAGGCTCCTTGATTTTGATCGGTGGGGATCCAGGGATCGGAAAATCGACGCTGCT
CTTGCAAGTTTCTCAGCAATTAGCAGCGATCGGCGGGACGGTTTTGTATGTGTCAGGAGAAGAAAGTGCCGAGCAGATCA
AAATGCGGGCGCAGCGCTTAGGCGCCATCGACACAGAATTTTATCTCTATGGGGAAACGGACATGTCAGAGATCAGTAAA
GCGATCGAAAACATCCGCCCAGATTATGTGATCATCGATTCGATCCAAACCATGACCCAACCGGATATCACCAGCGTGGC
AGGCAGTGTCAGCCAAGTGCGAGAAACGACCGCAGAATTGCTGAAAATCGCCAAGACCAATGGCATCGCCATCTTTATCG
TTGGCCATGTGACGAAGGAAGGCTCGATCGCAGGACCACGGATGTTGGAGCATATGGTGGATACCGTCTTGTATTTCGAA
GGCGAAAAGCACCACAGCTTTCGAATTTTGCGGGCCGTAAAAAACCGCTTTGGGTCCACCAATGAGATCGGGATTTTTGA
AATGGAAGAAGGCGGGTTGAAAGAAGTCGCCAATCCGTCACAAGTCTTTTTGGAAGAACGCTTAGAAGGCGCAACTGGAT
CGGCGATCGTAGTGGCAATGGAAGGCAGTCGGCCAATCTTGGTGGAAATCCAAGCACTGGTCACGCCAACGATGTTCGGC
AATGCCAAACGCACGACGACGGGGCTAGACTTCAACCGCGTCTCGCTGATCATGGCGGTTTTAGAAAAACGGGCAGGCTT
GCTGCTGCAAAATCAAGATGCCTACTTAAAAGCAGCCGGCGGGGTCAAATTAAATGAACCCGCGATCGATCTAGCGATCG
CTGTTAGTATCGCGTCTAGCTACAAAGAAAAAGGCACCAGCCCGACGGAGTGTTTTATCGGTGAAATTGGCTTGACGGGT
GAGATTCGCCGAGTCAACGCGATCGAGCAACGGGTCAAAGAGGTGCAAAAACTAGGTTTTACGAAAGTTTATTTGCCTAA
AAATAATCTGACTGGCTGGCAGCCGCCCAAAGGCATCGAAGTCGAAGGCGTGTCTACCTTAGCCGAAACCTTGCGGAAAG
TCTTCCGTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

AAA domain (AAA_25)

(71-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A242CQN0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.725

99.781

0.746
  radA Streptococcus mitis SK321

74.505

99.781

0.743
  radA Streptococcus pneumoniae Rx1

74.505

99.781

0.743
  radA Streptococcus pneumoniae D39

74.505

99.781

0.743
  radA Streptococcus pneumoniae R6

74.505

99.781

0.743
  radA Streptococcus pneumoniae TIGR4

74.505

99.781

0.743
  radA Bacillus subtilis subsp. subtilis str. 168

67.184

98.904

0.664