Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EGM181_RS00255 Genome accession   NZ_CP050485
Coordinates   22682..24052 (+) Length   456 a.a.
NCBI ID   WP_003129268.1    Uniprot ID   A0A2A4D851
Organism   Enterococcus gallinarum strain EGM181     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 17682..29052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGM181_RS00225 (EGM181_00230) - 18166..18984 (+) 819 WP_003129261.1 ZIP family metal transporter -
  EGM181_RS00230 (EGM181_00235) - 19026..19229 (-) 204 WP_003129262.1 hypothetical protein -
  EGM181_RS00235 (EGM181_00240) - 19374..19769 (+) 396 WP_103300721.1 DUF4809 family protein -
  EGM181_RS00240 (EGM181_00245) - 19858..21102 (-) 1245 WP_103300720.1 glutamate-5-semialdehyde dehydrogenase -
  EGM181_RS00245 (EGM181_00250) proB 21099..21908 (-) 810 WP_005473069.1 glutamate 5-kinase -
  EGM181_RS00250 (EGM181_00255) - 22120..22629 (+) 510 WP_005473067.1 dUTP diphosphatase -
  EGM181_RS00255 (EGM181_00260) radA 22682..24052 (+) 1371 WP_003129268.1 DNA repair protein RadA Machinery gene
  EGM181_RS00260 (EGM181_00265) - 24255..25382 (+) 1128 WP_103300719.1 PIN/TRAM domain-containing protein -
  EGM181_RS00265 (EGM181_00270) gltX 25560..27017 (+) 1458 WP_103300718.1 glutamate--tRNA ligase -
  EGM181_RS00270 (EGM181_00275) epsC 27277..27822 (+) 546 WP_003129273.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49646.36 Da        Isoelectric Point: 8.5560

>NTDB_id=433238 EGM181_RS00255 WP_003129268.1 22682..24052(+) (radA) [Enterococcus gallinarum strain EGM181]
MAKKAKSQFVCQNCGYSSPKYLGKCPNCGSWNSMVEEVIQETTDRRVRTTLTGQRMQPTKIADVIPKKEPRVQTKLAELN
RVLGGGVVPGSLILIGGDPGIGKSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKMRAQRLGAIDTEFYLYPETDMSEISK
AIENLTPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKLAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEKHHSFRILRAVKNRFGSTNEIGIFEMEAGGLKEVANPSQVFLEERLEGATGSAIVVAMEGSRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTSPTECFIGEIGLTG
EIRRVNAIEQRVKEVQKLGFTKVYLPKNNLTGWQPPKGIEVEGVSTLSETLRKVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=433238 EGM181_RS00255 WP_003129268.1 22682..24052(+) (radA) [Enterococcus gallinarum strain EGM181]
ATGGCTAAGAAAGCAAAGAGTCAGTTTGTCTGCCAAAATTGCGGATATTCTTCACCAAAGTATTTAGGGAAGTGCCCTAA
TTGTGGTAGTTGGAATTCGATGGTAGAAGAAGTGATTCAAGAAACAACGGATCGACGAGTACGGACCACTCTTACGGGAC
AACGGATGCAACCAACAAAAATTGCTGATGTCATACCAAAGAAAGAACCACGAGTACAGACCAAGCTAGCAGAGCTAAAC
CGAGTTTTGGGTGGTGGTGTTGTTCCGGGATCATTGATTTTGATTGGTGGTGATCCAGGGATCGGCAAGTCGACTCTTTT
ACTGCAAGTCTCACAGCAGCTTGCAGCTATTGGCGGCAAAGTATTGTATGTTTCGGGTGAGGAAAGTGCAGAGCAGATAA
AGATGCGTGCCCAGCGTTTAGGAGCCATTGATACGGAATTTTATTTATATCCAGAAACGGATATGTCTGAGATAAGCAAG
GCAATCGAAAATCTAACACCGGATTATGTCATCATAGATTCGATACAAACGATGACACAACCAGATATCACTAGTGTGGC
AGGAAGCGTGAGTCAGGTTCGAGAAACGACTGCCGAACTTCTAAAACTGGCTAAAACCAACGGGATTGCTATCTTTATTG
TGGGACATGTAACCAAAGAAGGTTCAATTGCTGGTCCACGAATGCTAGAGCACATGGTGGACACCGTTTTATATTTTGAA
GGTGAAAAGCATCACAGCTTCCGTATTTTGCGTGCCGTCAAAAATCGATTTGGTTCGACAAATGAGATTGGGATTTTTGA
AATGGAAGCAGGCGGATTGAAAGAAGTAGCCAACCCCTCTCAAGTCTTTCTTGAGGAGCGATTAGAAGGCGCTACGGGTT
CTGCCATCGTTGTTGCAATGGAAGGCAGTCGACCGATTTTAGTAGAGATCCAAGCTTTGGTGACACCAACTATGTTTGGT
AACGCTAAACGAACGACTACCGGATTGGATTTTAATCGTGTTTCATTAATCATGGCGGTCCTTGAAAAACGGGCTGGGTT
GCTTCTACAGAATCAAGATGCTTATTTAAAAGCAGCTGGTGGTGTAAAATTAAATGAACCTGCAATCGATTTAGCAATTG
CAGTCAGCATAGCCTCAAGTTATAAGGAAAAAGGTACGAGTCCTACGGAATGTTTTATCGGTGAAATTGGGTTGACTGGT
GAGATTCGACGTGTCAATGCGATCGAACAACGGGTAAAAGAAGTGCAAAAATTAGGATTTACTAAAGTATATTTACCTAA
AAATAATCTTACTGGGTGGCAACCGCCCAAAGGTATCGAAGTAGAAGGTGTGTCAACTTTATCGGAAACCCTACGAAAAG
TTTTTCGTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A4D851

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.286

99.781

0.741
  radA Streptococcus mitis SK321

74.066

99.781

0.739
  radA Streptococcus pneumoniae Rx1

74.066

99.781

0.739
  radA Streptococcus pneumoniae D39

74.066

99.781

0.739
  radA Streptococcus pneumoniae R6

74.066

99.781

0.739
  radA Streptococcus pneumoniae TIGR4

74.066

99.781

0.739
  radA Bacillus subtilis subsp. subtilis str. 168

66.297

98.904

0.656