Detailed information NTG0432858

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: G4G28_RS05155
• Genome accession: NZ_CP050454
• Coordinates: 1116008..1117132 (-)
• Length: 374 a.a.
• NCBI ID: WP_182991111.1
• Uniprot ID: A0A7G5ZBE3
• Organism: Massilia sp. Dwa41.01b
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1111008..1122132

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G4G28_RS05130 (G4G28_05125)	-	1111053..1111757 (-)	705	WP_182991107.1	LytTR family DNA-binding domain-containing protein	-
G4G28_RS05135 (G4G28_05130)	-	1111754..1112839 (-)	1086	WP_182991108.1	sensor histidine kinase	-
G4G28_RS05140 (G4G28_05135)	rpoH	1112999..1113889 (-)	891	WP_182991109.1	RNA polymerase sigma factor RpoH	-
G4G28_RS05145 (G4G28_05140)	ftsX	1114357..1115277 (-)	921	WP_183108108.1	permease-like cell division protein FtsX	-
G4G28_RS05150 (G4G28_05145)	-	1115274..1115987 (-)	714	WP_182991110.1	cell division ATP-binding protein FtsE	-
G4G28_RS05155 (G4G28_05150)	pilA	1116008..1117132 (-)	1125	WP_182991111.1	signal recognition particle-docking protein FtsY	Machinery gene
G4G28_RS05160 (G4G28_05155)	-	1117362..1118135 (-)	774	WP_182991112.1	DUF4261 domain-containing protein	-
G4G28_RS05165 (G4G28_05160)	-	1118400..1120109 (-)	1710	WP_182991113.1	thiamine pyrophosphate-binding protein	-
G4G28_RS05170 (G4G28_05165)	-	1120327..1121217 (+)	891	WP_183108109.1	hypothetical protein	-
G4G28_RS05175 (G4G28_05170)	-	1121281..1121772 (+)	492	WP_182991116.1	YcxB family protein	-

Sequence

Protein

• Length: 374 a.a.
• Molecular weight: 40028.29 Da
• Isoelectric Point: 5.8329

>NTDB_id=432858 G4G28_RS05155 WP_182991111.1 1116008..1117132(-) (pilA) [Massilia sp. Dwa41.01b]
MFSFFKRKKAAPETPAPAQDLPVPDRPVVDSPAEDIAAAPVAETPAPGFTRALAPISEAALFIEETAERPTTEAEKKKSW
MARLKAGLSKTSSNLSLLFVGARIDEDLYEELESALLMSDAGMDATQFLLDNLRRKVKEDKLLDAAAVKAALKQLMVDML
RPLEKPLELGRHEPLVMMIAGVNGAGKTTTIGKLAKHMQRSSQSVLLAAGDTFRAAAREQLMVWGQRNNVTVISQASGDP
AAVAFDAVQSGKARGVDVVMVDTAGRLPTQLHLMEELKKIKRVIGKGMDGAPHETLLVIDGNTGQNALAQVKAFDDALQL
TGLVITKLDGTAKGGVIAAIARTRPVPVYFIGVGEKLDDLQPFSADEFVEALLG

Nucleotide

• Length: 1125 bp

>NTDB_id=432858 G4G28_RS05155 WP_182991111.1 1116008..1117132(-) (pilA) [Massilia sp. Dwa41.01b]
ATGTTTAGTTTCTTCAAGAGAAAAAAAGCTGCCCCCGAAACGCCGGCACCGGCGCAGGACCTGCCCGTCCCGGATCGGCC
TGTAGTGGACTCGCCTGCCGAGGATATCGCCGCCGCGCCTGTGGCGGAAACGCCGGCGCCTGGCTTCACCCGCGCCCTGG
CCCCGATCTCCGAAGCGGCCCTTTTCATCGAGGAAACGGCCGAGCGCCCGACCACCGAGGCGGAGAAGAAAAAATCCTGG
ATGGCGCGCCTGAAGGCCGGCCTGTCCAAGACCTCGAGCAACCTGTCGCTGCTGTTCGTCGGTGCGCGCATCGACGAAGA
CCTGTACGAGGAACTCGAGTCCGCGCTCCTGATGTCGGACGCAGGCATGGACGCCACCCAGTTCCTGCTCGATAACCTGC
GCCGCAAGGTCAAGGAAGACAAGCTGCTCGACGCCGCTGCCGTCAAGGCCGCACTGAAGCAGCTCATGGTCGACATGCTG
CGCCCGCTGGAAAAACCGCTGGAGCTGGGCCGCCACGAGCCGCTGGTGATGATGATCGCGGGCGTCAACGGCGCCGGCAA
GACCACCACCATCGGCAAGCTCGCCAAGCACATGCAGCGCTCCAGCCAATCGGTGCTGCTGGCCGCCGGCGACACTTTCC
GCGCCGCTGCGCGCGAGCAGCTGATGGTCTGGGGCCAGCGCAACAACGTCACCGTGATTTCCCAGGCATCGGGCGACCCG
GCTGCCGTCGCCTTCGATGCCGTGCAGTCGGGCAAAGCGCGCGGCGTCGACGTCGTCATGGTCGATACCGCCGGCCGGCT
GCCGACCCAGCTGCACCTGATGGAAGAGCTGAAGAAGATCAAGCGCGTGATCGGCAAGGGCATGGATGGCGCCCCGCACG
AAACCCTGCTCGTCATCGACGGCAATACCGGCCAGAACGCGCTGGCGCAAGTGAAAGCCTTCGACGATGCGCTGCAATTG
ACCGGCCTGGTCATCACCAAGCTCGACGGTACTGCCAAGGGCGGCGTCATCGCCGCAATCGCCCGCACCCGTCCGGTACC
GGTGTACTTCATCGGCGTGGGCGAAAAGCTGGACGACCTGCAGCCTTTCAGTGCAGACGAGTTCGTTGAAGCGCTGCTGG
GATAA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (175-374)

• SRP54-type protein, helical bundle domain (SRP54_N) (101-154)

3D structure

• AlphaFold DB: A0A7G5ZBE3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	51.551	100	0.578