Detailed information NTG0430672

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: G9457_RS20370
• Genome accession: NZ_CP050187
• Coordinates: 4266206..4267081 (+)
• Length: 291 a.a.
• NCBI ID: WP_005314162.1
• Uniprot ID: A2T195
• Organism: Aeromonas salmonicida subsp. masoucida strain RZ6S-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4261206..4272081

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G9457_RS20345 (G9457_20400)	-	4261564..4261947 (+)	384	WP_088814130.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
G9457_RS20350 (G9457_20405)	-	4261992..4262909 (+)	918	WP_088814129.1	IS5-like element ISAs4 family transposase	-
G9457_RS20355 (G9457_20410)	-	4262953..4263063 (+)	111	Protein_3965	pilus assembly protein TapA	-
G9457_RS20360 (G9457_20415)	pilB	4263067..4264773 (+)	1707	WP_088814128.1	PilB family type IVa pilus assembly ATPase TapB	Machinery gene
G9457_RS20365 (G9457_20420)	-	4265154..4266142 (+)	989	Protein_3967	type II secretion system F family protein	-
G9457_RS20370 (G9457_20425)	pilD	4266206..4267081 (+)	876	WP_005314162.1	A24 family peptidase	Machinery gene
G9457_RS20375 (G9457_20430)	coaE	4267101..4267715 (+)	615	WP_042468303.1	dephospho-CoA kinase	-
G9457_RS20380 (G9457_20435)	zapD	4267753..4268475 (+)	723	WP_005314166.1	cell division protein ZapD	-
G9457_RS20385 (G9457_20440)	yacG	4268485..4268679 (+)	195	WP_005314167.1	DNA gyrase inhibitor YacG	-
G9457_RS20390 (G9457_20445)	mutT	4268756..4269163 (-)	408	WP_005314168.1	8-oxo-dGTP diphosphatase MutT	-
G9457_RS20395 (G9457_20450)	-	4269172..4270356 (-)	1185	WP_042468305.1	tetratricopeptide repeat protein	-

Sequence

Protein

• Length: 291 a.a.
• Molecular weight: 32500.78 Da
• Isoelectric Point: 6.9383

>NTDB_id=430672 G9457_RS20370 WP_005314162.1 4266206..4267081(+) (pilD) [Aeromonas salmonicida subsp. masoucida strain RZ6S-1]
MTLLLELAHGLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYRSYFSSDTPQPEDDERYNLMVPRSCCPRCNH
PITALENIPLLSWLWLKGRCRGCQAAISARYPLVELLTALLSVVVAMTLTPGWGTLAALLLTWVLVALTFIDLDKMLLPD
QLTLPLLWGGLLFNLLGGYVPLGDAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSS
LVGAIFGIGLILLRNHHQSKPIPFGPYLAIAGWIALLWGDSITRWYLSTIL

Nucleotide

• Length: 876 bp

>NTDB_id=430672 G9457_RS20370 WP_005314162.1 4266206..4267081(+) (pilD) [Aeromonas salmonicida subsp. masoucida strain RZ6S-1]
ATGACACTTCTTCTCGAGCTGGCACACGGCTTGCCATGGCTCTACTTCTCGCTGGTTTTTCTCTTCTCCCTGATGATCGG
CAGCTTTCTCAACGTGGTGATCCACCGCCTGCCCATCATGCTGGAACGGGAGTGGCAGGCCGAATATCGCAGCTATTTCA
GTTCAGACACGCCGCAGCCAGAAGATGACGAGCGCTACAATCTGATGGTGCCGCGCTCCTGCTGCCCTCGCTGCAATCAC
CCCATAACGGCCTTGGAAAACATTCCCCTGCTGAGCTGGCTCTGGCTCAAAGGTCGCTGCCGGGGCTGCCAGGCCGCCAT
TTCGGCTCGCTATCCGCTGGTGGAGCTGCTGACCGCGCTGCTCTCTGTGGTGGTTGCCATGACGCTCACCCCTGGCTGGG
GCACGCTGGCTGCCCTCCTGCTGACCTGGGTGCTGGTGGCGCTCACCTTTATCGATCTGGACAAGATGCTGCTGCCGGAC
CAGCTCACCTTGCCGCTACTGTGGGGCGGGCTGCTGTTCAACCTGCTGGGCGGATACGTGCCTCTCGGCGATGCTGTCAT
CGGCGCCATGGCAGGCTATCTGGTGCTGTGGAGCCTCTACTGGGCCTTCAAGCTGCTTACCGGCAAGGAGGGCATGGGCT
ATGGGGACTTCAAACTGCTGGCAGCGCTGGGAGCCTGGCTTGGCTGGCAGGCGCTGCCCATAGTACTGCTGCTCTCTTCT
CTGGTCGGCGCCATCTTCGGCATTGGCCTCATTTTGCTGCGCAACCATCACCAGAGCAAACCCATCCCCTTTGGCCCCTA
TCTCGCCATCGCAGGCTGGATCGCACTGCTCTGGGGCGATAGCATCACCCGCTGGTATCTCTCCACTATCCTCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (140-248)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-128)

3D structure

• AlphaFold DB: A2T195

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	60.764	98.969	0.601
pilD	Vibrio campbellii strain DS40M4	55.556	98.969	0.55
pilD	Acinetobacter baumannii D1279779	55.39	92.44	0.512
pilD	Acinetobacter nosocomialis M2	55.39	92.44	0.512
pilD	Neisseria gonorrhoeae MS11	50.746	92.096	0.467