Detailed information NTG0430065

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: IF725_RS04145
• Genome accession: NZ_CP062412
• Coordinates: 837366..839774 (-)
• Length: 802 a.a.
• NCBI ID: WP_000560851.1
• Uniprot ID: -
• Organism: Staphylococcus aureus strain NAS_AN_181
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 832366..844774

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IF725_RS04120 (IF725_04110)	rsmB	832525..833832 (-)	1308	WP_000572241.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
IF725_RS04125 (IF725_04115)	fmt	833829..834764 (-)	936	WP_000161293.1	methionyl-tRNA formyltransferase	-
IF725_RS04130 (IF725_04120)	-	834757..835245 (-)	489	WP_000985294.1	peptide deformylase	-
IF725_RS04135 (IF725_04125)	-	835544..835747 (+)	204	WP_001198020.1	TM2 domain-containing protein	-
IF725_RS04140 (IF725_04130)	-	835910..836863 (-)	954	WP_000751704.1	hypothetical protein	-
IF725_RS04145 (IF725_04135)	priA	837366..839774 (-)	2409	WP_000560851.1	primosomal protein N'	Machinery gene
IF725_RS04150 (IF725_04140)	coaBC	839774..840973 (-)	1200	WP_000722165.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
IF725_RS04155 (IF725_04145)	rpoZ	841189..841407 (-)	219	WP_000933956.1	DNA-directed RNA polymerase subunit omega	-
IF725_RS04160 (IF725_04150)	gmk	841407..842030 (-)	624	WP_000368226.1	guanylate kinase	-
IF725_RS04165 (IF725_04155)	-	842305..844002 (+)	1698	WP_000312767.1	NFACT family protein	-
IF725_RS04170 (IF725_04160)	-	844265..844666 (-)	402	WP_000354184.1	VOC family protein	-

Sequence

Protein

• Length: 802 a.a.
• Molecular weight: 92566.46 Da
• Isoelectric Point: 6.7335

>NTDB_id=430065 IF725_RS04145 WP_000560851.1 837366..839774(-) (priA) [Staphylococcus aureus strain NAS_AN_181]
MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRTIQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTS
ELIALSEWMGSTHVIKRISMLEVMLPSAIKAKYKKAFKMKDDIEVPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
IVEERTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFLTDIEDMGFSKSSLDGLIKKGYV
EKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAIKAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLV
PEIALTPQMVLRFKRRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHESTYKQEDY
PRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVNQQALPEIDIVDMREELSEGNRSMFSKDLRE
AIQLRLDRQEQVVLFLNRRGYASFMLCRDCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGT
GTQKVEELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITLVGVLNADTMLNLPDFR
ASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQKNDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKV
MEASQHVHKILLQHLTEKALVLGPSPAALARINNEFRFQILVKYKSEPGLLKAIQFLDDYYHEKFIKEKLALKIDIDPQM
MM

Nucleotide

• Length: 2409 bp

>NTDB_id=430065 IF725_RS04145 WP_000560851.1 837366..839774(-) (priA) [Staphylococcus aureus strain NAS_AN_181]
ATGATAGCGAAAGTCATAGTCGATGTCGCGTCGAAGAGCGTTGACTATAAATTTGATTATATAATTCCTGAACAACTCGA
ATCTGTCATCCAACCTGGTGTGCGTGTGATTGTACCTTTTGGACCAAGAACGATTCAAGGTTATGTAATGGAAGTAACAG
CAGAACCTGATGCACAACTTGACGTTTCGAAGTTAAAAAAAATCATAGAAGTGAAAGATATACAACCTGAATTAACATCA
GAATTAATAGCTTTAAGTGAGTGGATGGGTTCAACTCATGTCATTAAACGTATTTCTATGCTAGAAGTGATGCTTCCGAG
TGCTATTAAAGCGAAGTATAAAAAAGCATTTAAGATGAAAGATGACATAGAGGTACCTTCAGCTTTATTACAAAAATTTG
ATAAGCATGGTTACTATTATTATAAAGATGCGCAAAAAAATAATGATATTCAATTGCTTATGAAGTTGTTAAAAGATGAT
ATCGTTGAAGAAAGAACGATTCTAACACAAAATATAACTAAAAAAACCAAGCGTGCTGTTCGTGTCATTGAAGGGTATCA
TCCTGATGAAGTATTAGCTAAGTTGGAGAAAGTTATTAAACAATACGATTTGTATGCTTACTTGTCTGAAGAACAACATA
AAACAATATTTTTAACTGATATTGAGGATATGGGCTTTTCAAAATCCAGTTTAGATGGACTTATCAAAAAAGGTTATGTT
GAAAAATATGACGCAGTTGTTGAAAGAGACCCATTTAAAGATCGTGTTTTCGAACAAGAATCAAAACAGCAATTAACAGA
AGACCAATATAAAGCATATGAAGCGATTAAAGCTAAAATTGTAAGCCAAGAGCAAGAAACATTTTTACTTCATGGTGTGA
CGGGATCAGGTAAAACAGAAGTATATTTACAAACGATAGAAGATGTTTTAAGTCAAGGAAAACAGGCGATGATGTTAGTT
CCTGAAATCGCTCTAACACCGCAAATGGTTTTACGCTTCAAACGTCGATTTGGTGATGACGTTGCTGTATTACATTCTGG
CTTATCTAATGGGGAACGTTATGATGAGTGGCAAAAAATAAGGGATGGTCGTGCGAGAGTAAGTGTTGGTGCAAGGTCAA
GTGTGTTCGCACCTTTCAAAAATTTAGGGTTAATCATCATTGATGAAGAACATGAATCTACATATAAGCAAGAAGATTAT
CCGAGATATCACGCTAGAGAAATTGCCCAATGGCGAAGTGAATATCATCACTGCCCAGTCATTTTAGGAAGTGCAACACC
ATGTCTTGAAAGTTATGCACGAGCTGAAAAAGGCGTTTATCATTTGCTATCATTACCAAACAGAGTGAACCAACAAGCTT
TACCTGAAATTGATATAGTAGACATGCGTGAAGAATTGAGTGAAGGTAATCGGTCAATGTTTTCAAAAGATTTACGTGAA
GCCATACAATTAAGATTAGATCGACAAGAACAAGTTGTTTTATTTTTAAATCGACGTGGTTATGCATCATTTATGTTATG
TCGGGATTGTGGTTATGTACCGCAATGCCCAAACTGTGATATTTCATTAACGTATCATAAAACGACAGACTTATTAAAAT
GTCACTATTGTGGTTACCAAGAGACGCCACCGAATCAATGTCCAAATTGTGAGAGTGAACACATTCGACAAGTAGGTACT
GGTACTCAGAAAGTTGAAGAACTGTTGCAACAAGAATTTGAAGATGCGCGCATAATTAGGATGGATGTAGATACAACCTC
TAAGAAAGGTGCACATGAAAAGTTATTAACTGAATTCGAAAAAGGTAACGGTGACATTTTACTAGGTACCCAGATGATTG
CGAAAGGATTAGATTATCCAAATATCACTTTAGTTGGTGTGCTGAATGCAGATACAATGTTAAATTTACCTGATTTTCGG
GCGAGCGAACGCACTTATCAACTATTAACGCAAGTGGCTGGTAGAGCTGGTCGTCATGAAAAGGCAGGTCAAGTCATCAT
TCAAACGTATAATCCAGACCATTATTCAATATTGGATGTTCAAAAAAATGATTATTTAACATTTTATCGTCAGGAAATGG
AATATCGTAAATTAGGAAAGTATCCACCGTATTATTATTTGATTAATTTCACAATCTCACATAAAGAAATGAAGAAGGTT
ATGGAAGCATCGCAACATGTTCATAAAATTTTATTACAGCATTTAACAGAAAAAGCGCTTGTACTAGGCCCATCTCCGGC
AGCACTTGCGAGAATCAACAATGAATTTAGATTCCAAATTTTAGTGAAATATAAAAGTGAACCTGGATTATTAAAAGCCA
TTCAATTTTTAGATGACTATTACCATGAAAAATTTATAAAAGAAAAATTAGCATTGAAGATTGATATTGATCCACAGATG
ATGATGTAA

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (4-106)

• Primosomal protein N C-terminal domain (PriA_C) (703-798)

• Helicase conserved C-terminal domain (Helicase_C) (564-659)

• Type III restriction enzyme, res subunit (ResIII) (264-427)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (516-541)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	50.433	100	0.509