Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NCK_RS03715 Genome accession   NZ_CP050078
Coordinates   776635..777615 (+) Length   326 a.a.
NCBI ID   WP_153257845.1    Uniprot ID   A0A3S4M510
Organism   Citrobacter koseri strain NJ     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 771635..782615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NCK_RS03685 (NCK_03690) ansB 772146..773192 (+) 1047 WP_049010789.1 L-asparaginase 2 -
  NCK_RS03690 (NCK_03695) hemW 773311..774447 (-) 1137 WP_219936608.1 radical SAM family heme chaperone HemW -
  NCK_RS03695 (NCK_03700) - 774440..775033 (-) 594 WP_012135070.1 XTP/dITP diphosphatase -
  NCK_RS03700 (NCK_03705) yggU 775041..775331 (-) 291 WP_012135069.1 DUF167 family protein YggU -
  NCK_RS03705 (NCK_03710) - 775328..775894 (-) 567 WP_012135068.1 YggT family protein -
  NCK_RS03710 (NCK_03715) - 775913..776617 (-) 705 WP_112001564.1 YggS family pyridoxal phosphate-dependent enzyme -
  NCK_RS03715 (NCK_03720) pilT 776635..777615 (+) 981 WP_153257845.1 type IV pilus twitching motility protein PilT Machinery gene
  NCK_RS03720 (NCK_03725) ruvX 777765..778181 (-) 417 WP_153257844.1 Holliday junction resolvase RuvX -
  NCK_RS03725 (NCK_03730) - 778181..778744 (-) 564 WP_012135063.1 YqgE/AlgH family protein -
  NCK_RS03730 (NCK_03735) gshB 778921..779868 (-) 948 WP_012135062.1 glutathione synthase -
  NCK_RS03735 (NCK_03740) rsmE 779881..780612 (-) 732 WP_049010798.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NCK_RS03740 (NCK_03745) endA 780690..781397 (-) 708 WP_153259022.1 deoxyribonuclease I -
  NCK_RS03745 (NCK_03750) - 781492..781989 (-) 498 WP_024130936.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35675.72 Da        Isoelectric Point: 6.9259

>NTDB_id=429918 NCK_RS03715 WP_153257845.1 776635..777615(+) (pilT) [Citrobacter koseri strain NJ]
MDMEEIVALSVKHNVSDLHLCNAWPARWRKRGKVEIAPFSSPNVEVLLSQWLNDAQRRAWQADGQLDFAVSLPGELRLRA
SAFHHEQGASLALRLLPTTCPQLDALQTPAALPALLGGEHGLILVTGATGSGKSTTLAAMVEYLNQHLDGHILTLEDPIE
YRHTSQRCLIQQREVGVHCRSFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRSQLAGSLRAVLSQKLETDRQDGRVALFELLINTPAVGNLIREGKIHQLPGVIQTSQQAGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=429918 NCK_RS03715 WP_153257845.1 776635..777615(+) (pilT) [Citrobacter koseri strain NJ]
ATGGATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAACGCATGGCCTGCGCG
CTGGCGTAAACGGGGAAAAGTGGAAATAGCCCCGTTTAGCAGCCCGAATGTCGAGGTTTTGCTGTCACAATGGCTTAACG
ACGCGCAGCGCCGGGCGTGGCAGGCGGATGGTCAGCTCGACTTTGCGGTGTCGCTGCCGGGCGAACTGCGGTTACGCGCC
AGCGCTTTTCATCACGAGCAGGGCGCATCGCTGGCGCTGCGGTTGCTGCCCACTACGTGCCCGCAACTGGACGCGCTCCA
GACGCCTGCCGCGCTACCCGCGCTCCTTGGCGGAGAGCACGGACTGATTCTGGTGACCGGAGCGACGGGAAGTGGGAAAT
CCACCACGCTGGCGGCAATGGTGGAATACCTTAACCAGCATCTCGATGGTCATATTCTGACGCTGGAAGATCCTATTGAG
TACCGCCATACCAGCCAGCGCTGCCTGATTCAGCAGCGGGAGGTTGGCGTACACTGCCGCTCCTTTGCCGCCGGGTTACG
CGCTGCGCTGCGTGAAGACCCGGATGTGATTTTATTAGGCGAACTGCGCGACAGTGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTTCTGGCGACGCTACATACGCGCGGCGCGGCACAGGCGGTAGAGCGGCTGGTTGACTCC
TTTCCGGCGCAGGAGAAAGATCCGGTACGCAGTCAACTTGCGGGCAGCCTGCGCGCCGTGTTGTCGCAGAAGCTGGAAAC
CGACAGGCAGGACGGACGCGTGGCGTTATTTGAACTGCTCATCAACACGCCAGCGGTTGGCAATCTTATTCGGGAGGGGA
AGATTCACCAGTTGCCCGGCGTTATTCAGACCAGCCAACAGGCGGGGATGCAGACGTTTGCCCAGAGTCTGCAACAGCGG
CAGGCGCAGGGCAGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(52-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S4M510

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.07

100

0.512
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.07

100

0.512
  pilT Neisseria meningitidis 8013

49.39

100

0.497
  pilT Neisseria gonorrhoeae MS11

49.085

100

0.494
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Pseudomonas stutzeri DSM 10701

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Legionella pneumophila strain ERS1305867

46.584

98.773

0.46
  pilT Legionella pneumophila strain Lp02

46.584

98.773

0.46
  pilU Pseudomonas stutzeri DSM 10701

40.909

100

0.414
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.752

98.773

0.393
  pilU Acinetobacter baylyi ADP1

38.58

99.387

0.383