Detailed information NTG0429824

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HAP33_RS23330
• Genome accession: NZ_CP050054
• Coordinates: 5059452..5060324 (+)
• Length: 290 a.a.
• NCBI ID: WP_003112839.1
• Uniprot ID: P22610
• Organism: Pseudomonas aeruginosa strain LIUYANG-A
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5054452..5065324

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HAP33_RS23305	nadC	5054793..5055641 (+)	849	WP_003104915.1	carboxylating nicotinate-nucleotide diphosphorylase	-
HAP33_RS23315	pilA	5055843..5056292 (-)	450	WP_003112842.1	type 4a pilus biogenesis protein PilA	Machinery gene
HAP33_RS23320	pilB	5056524..5058224 (+)	1701	WP_003112841.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HAP33_RS23325	pilC	5058228..5059448 (+)	1221	WP_003112840.1	type 4a pilus biogenesis protein PilC	Machinery gene
HAP33_RS23330	pilD	5059452..5060324 (+)	873	WP_003112839.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
HAP33_RS23335	coaE	5060321..5060932 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
HAP33_RS23340	yacG	5060929..5061129 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
HAP33_RS23345	-	5061166..5061375 (-)	210	WP_003094660.1	hypothetical protein	-
HAP33_RS23350	-	5061481..5062170 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
HAP33_RS23355	-	5062167..5062637 (-)	471	WP_003103866.1	hypothetical protein	-
HAP33_RS23360	-	5062634..5063059 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
HAP33_RS23365	-	5063192..5063821 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
HAP33_RS23370	-	5063818..5064267 (+)	450	WP_003094670.1	MOSC domain-containing protein	-
HAP33_RS23375	-	5064293..5064463 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31869.92 Da
• Isoelectric Point: 7.7068

>NTDB_id=429824 HAP33_RS23330 WP_003112839.1 5059452..5060324(+) (pilD) [Pseudomonas aeruginosa strain LIUYANG-A]
MPLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=429824 HAP33_RS23330 WP_003112839.1 5059452..5060324(+) (pilD) [Pseudomonas aeruginosa strain LIUYANG-A]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCTAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

3D structure

• AlphaFold DB: P22610

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.357	96.552	0.534
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	50.19	90.69	0.455