Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VFU_RS04275 Genome accession   NC_016602
Coordinates   889768..891456 (-) Length   562 a.a.
NCBI ID   WP_014204460.1    Uniprot ID   -
Organism   Vibrio furnissii NCTC 11218     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 884768..896456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VFU_RS04250 (vfu_A00866) yacG 885903..886100 (-) 198 WP_004729092.1 DNA gyrase inhibitor YacG -
  VFU_RS04255 (vfu_A00867) zapD 886158..886898 (-) 741 WP_004729090.1 cell division protein ZapD -
  VFU_RS04260 (vfu_A00868) coaE 886931..887539 (-) 609 WP_014204457.1 dephospho-CoA kinase -
  VFU_RS04265 (vfu_A00869) pilD 887542..888411 (-) 870 WP_014204458.1 prepilin peptidase Machinery gene
  VFU_RS04270 (vfu_A00870) pilC 888463..889689 (-) 1227 WP_014204459.1 type II secretion system F family protein Machinery gene
  VFU_RS04275 (vfu_A00871) pilB 889768..891456 (-) 1689 WP_014204460.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VFU_RS04280 (vfu_A00872) - 891463..891912 (-) 450 WP_014204461.1 pilin -
  VFU_RS04285 (vfu_A00873) nadC 892159..893046 (-) 888 WP_014204462.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VFU_RS04290 (vfu_A00874) ampD 893197..893748 (+) 552 WP_014204463.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VFU_RS04295 (vfu_A00875) fldB 893770..894291 (-) 522 WP_004729075.1 flavodoxin FldB -
  VFU_RS04300 (vfu_A00876) xerD 894432..895340 (+) 909 WP_004729073.1 site-specific tyrosine recombinase XerD -
  VFU_RS04305 (vfu_A00877) dsbC 895357..896121 (+) 765 WP_014204464.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62084.14 Da        Isoelectric Point: 5.8037

>NTDB_id=42937 VFU_RS04275 WP_014204460.1 889768..891456(-) (pilB) [Vibrio furnissii NCTC 11218]
MLTNLIAILRQAELISPTQEQAVIDHIHASGATVPEALIDLAIFHHHDLTEQLSQIFGLPETDLTQFDYAPLCQQLGLRE
LITRYQALPLHSNGQILTLAVADPTHLQVEDEFRFATGLQVELVLADHKALHAAIRRLYGRSLATPGTQGKEISQDELAD
LIQISDDEKNTIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESHYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLDADTAIDMRVSTLPTLWGEKIVLRLLDSSAANLNIDKLGYNEQQKQLYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTEQVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAIKAAQTGHLVLSTLHTNSAAETVIRLANMGIEPFNLASSLSLIIAQRLARRLCPYCKVAITPPAALQQQFGLLAED
AVYEAHPSGCTECTNGYSGRVGIYEVMAFHPALADAVMQRASIHQIETIAQQHGMQPLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=42937 VFU_RS04275 WP_014204460.1 889768..891456(-) (pilB) [Vibrio furnissii NCTC 11218]
ATGCTGACCAACCTTATCGCCATTCTGCGTCAGGCTGAGTTGATCAGCCCAACGCAGGAACAGGCCGTAATCGATCATAT
TCACGCTTCCGGTGCCACGGTGCCGGAGGCGTTGATCGATTTGGCGATATTTCATCATCACGATTTAACTGAGCAACTAA
GTCAGATCTTTGGCCTACCCGAAACCGATCTCACTCAGTTTGATTACGCGCCATTGTGCCAGCAGTTGGGACTTCGAGAA
CTCATCACCCGCTACCAAGCCCTGCCGCTGCACAGCAATGGCCAAATCCTGACGTTGGCGGTTGCTGATCCAACTCACCT
GCAAGTCGAAGACGAGTTTCGCTTTGCCACCGGATTACAGGTGGAACTGGTGCTCGCCGATCATAAAGCGCTGCACGCCG
CCATTCGCCGCTTGTATGGGCGCAGCCTCGCGACGCCGGGGACGCAAGGCAAAGAGATCAGTCAGGATGAATTGGCGGAT
CTGATACAGATCTCTGACGATGAAAAAAACACCATCGAAGATCTCAGCCAAGATGATTCACCGGTCAGTCGTTTTATCAA
CCAGATCCTACTCGATGCCGTGCGCAAAGGTGCATCCGACATTCACTTTGAGCCGTATGAGTCCCACTACCGTATTCGTC
TGCGCTGTGACGGCATTCTGGTCGAAACCCAACAACCTGCCAGTCACCTCAGCCGACGCTTGGCAGCCCGAATCAAAATT
TTGTCGAAACTGGATATTGCCGAGCGCCGCCTCCCGCAAGATGGACGTATCAAACTGCGCTTGGATGCCGACACCGCGAT
TGATATGCGTGTATCGACCTTGCCAACATTGTGGGGTGAAAAGATTGTTCTGCGCTTACTCGATAGCAGCGCGGCCAACC
TCAACATCGACAAACTCGGCTACAACGAACAGCAGAAACAGCTCTACCTCGCGGCCCTTACCAAACCGCAAGGCATGATC
TTAATGACCGGCCCAACCGGCAGCGGCAAAACTGTGTCGCTCTACACCGGATTACGCATTCTCAATACCGAACAAGTGAA
CATCTCCACCGCCGAAGATCCGGTGGAAATCAACCTGCCCGGCATCAATCAGGTGCAAGTTCAACCCAAAATCGGCTTTG
GCTTTGCACAGGCGCTGCGCTCGTTTCTGCGTCAAGATCCCGATGTGGTGATGGTGGGAGAAATCCGCGATCTAGAAACG
GCAGAAATCGCCATTAAAGCCGCGCAAACCGGGCACTTGGTGCTCTCAACCTTGCACACCAACTCGGCCGCGGAAACCGT
GATTCGTTTAGCCAATATGGGCATTGAACCCTTTAACCTTGCCTCATCATTGAGCCTGATCATTGCGCAGCGGCTGGCCC
GCCGTCTGTGCCCGTATTGCAAGGTCGCCATCACACCTCCAGCTGCGCTGCAGCAGCAATTTGGCTTGCTCGCCGAAGAT
GCCGTGTACGAAGCACATCCATCGGGTTGCACGGAATGCACCAATGGGTATAGCGGCCGCGTGGGCATTTATGAAGTGAT
GGCGTTTCATCCCGCCTTGGCGGACGCGGTGATGCAGCGCGCCAGCATTCATCAAATCGAAACCATTGCCCAACAGCACG
GTATGCAGCCCTTGCTGCAATCTGGGATTGAAAAACTGCGCGCGGGCATCACCAGTTTTAGTGAATTGCAGCGAGTACTC
TACTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

82.028

100

0.82
  pilB Vibrio campbellii strain DS40M4

72.064

100

0.721
  pilB Vibrio parahaemolyticus RIMD 2210633

72.064

100

0.721
  pilB Legionella pneumophila strain ERS1305867

52.519

91.815

0.482
  pilB Acinetobacter baumannii D1279779

50.68

91.637

0.464
  pilB Acinetobacter baylyi ADP1

49.903

91.993

0.459
  pilF Neisseria gonorrhoeae MS11

44.858

100

0.45
  pilB Haemophilus influenzae 86-028NP

39.469

93.772

0.37
  pilB/pilB1 Synechocystis sp. PCC 6803

37.273

97.865

0.365
  pilF Thermus thermophilus HB27

36.477

100

0.365

Multiple sequence alignment