Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   G9462_RS02365 Genome accession   NZ_CP050020
Coordinates   503475..504776 (-) Length   433 a.a.
NCBI ID   WP_009893082.1    Uniprot ID   Q2T1C5
Organism   Burkholderia thailandensis strain BPM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 498475..509776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G9462_RS02350 (G9462_02350) nirD 499365..499760 (-) 396 WP_009893098.1 nitrite reductase small subunit NirD -
  G9462_RS02355 (G9462_02355) nirB 499836..502280 (-) 2445 WP_009893095.1 nitrite reductase large subunit NirB -
  G9462_RS02360 (G9462_02360) maiA 502673..503317 (+) 645 WP_009893084.1 maleylacetoacetate isomerase -
  G9462_RS02365 (G9462_02365) pilA 503475..504776 (-) 1302 WP_009893082.1 signal recognition particle-docking protein FtsY Machinery gene
  G9462_RS02370 (G9462_02370) - 504760..504954 (+) 195 WP_223297025.1 hypothetical protein -
  G9462_RS02375 (G9462_02375) rsmD 505034..505660 (+) 627 WP_019255510.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  G9462_RS02380 (G9462_02380) coaD 505769..506269 (+) 501 WP_004195249.1 pantetheine-phosphate adenylyltransferase -
  G9462_RS02385 (G9462_02385) - 506334..506597 (+) 264 WP_009893077.1 YfhL family 4Fe-4S dicluster ferredoxin -
  G9462_RS02390 (G9462_02390) hisC 506731..507798 (-) 1068 WP_165981531.1 histidinol-phosphate transaminase -
  G9462_RS02395 (G9462_02395) pth 507872..508477 (-) 606 WP_009893073.1 aminoacyl-tRNA hydrolase -
  G9462_RS02400 (G9462_02400) - 508591..509205 (-) 615 WP_025369612.1 50S ribosomal protein L25/general stress protein Ctc -

Sequence

Protein


Download         Length: 433 a.a.        Molecular weight: 45283.62 Da        Isoelectric Point: 5.3487

>NTDB_id=429277 G9462_RS02365 WP_009893082.1 503475..504776(-) (pilA) [Burkholderia thailandensis strain BPM]
MFSFFKRFKKAPDAAPAEPQQAPDAPQDAAGESAAPEAASKAARVDAPAAPVSRGEYSEAPATERPRSDAPAPVRATPPV
SPAAATPESPAGQPSAAEAARAPSVVMTVTPAANGRNGVVETVEIVPPPAPEPAAKKSWIARLKSGLAKTSSSITNVFVN
TKIDDALYEELETALLMSDAGVDATEHLLGALREKVRTGRLTDPQQVKDALRGLLVELLTPLEQSLVLGRAQPLVMMIAG
VNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAIWGERNNVTVVQQESGDPAAVIFDAVSAARARNIDVMMA
DTAGRLPTQLHLMEELRKVKRVIGKAHDGAPHEVLLVIDANTGQNALAQVKAFDDALGLTGLIVTKLDGTAKGGILAAIA
RQRPIPVYFIGVGEKVEDLQPFSAEEFADALLG

Nucleotide


Download         Length: 1302 bp        

>NTDB_id=429277 G9462_RS02365 WP_009893082.1 503475..504776(-) (pilA) [Burkholderia thailandensis strain BPM]
ATGTTCAGCTTCTTCAAACGATTCAAGAAAGCGCCCGACGCCGCACCGGCCGAGCCGCAGCAAGCGCCCGACGCGCCGCA
GGACGCGGCCGGCGAGTCGGCCGCGCCCGAGGCCGCGTCCAAGGCCGCGCGAGTCGACGCGCCCGCTGCGCCGGTCTCGC
GCGGCGAGTACAGCGAAGCGCCGGCTACCGAACGGCCCCGTTCCGACGCGCCGGCCCCCGTTCGCGCGACACCGCCCGTC
TCCCCCGCAGCAGCGACGCCGGAATCGCCGGCCGGGCAGCCGTCCGCCGCCGAAGCGGCGCGCGCGCCGTCCGTCGTGAT
GACGGTGACGCCGGCCGCCAACGGCCGCAACGGCGTCGTCGAGACGGTCGAGATCGTGCCGCCGCCCGCGCCCGAGCCGG
CCGCGAAGAAATCGTGGATCGCGCGGCTGAAGTCCGGCCTCGCGAAGACGAGCTCGAGCATCACGAACGTGTTCGTGAAC
ACGAAGATCGACGACGCGCTCTACGAGGAGCTCGAGACGGCGCTGCTGATGTCCGACGCCGGCGTCGACGCGACCGAGCA
TCTGCTCGGCGCGCTGCGCGAGAAGGTGCGCACGGGCCGCCTCACCGATCCGCAACAGGTGAAGGACGCGCTGCGCGGCC
TGCTCGTCGAGTTGCTGACGCCGCTCGAGCAGTCGCTCGTGCTCGGCCGCGCGCAGCCGCTCGTGATGATGATCGCGGGC
GTGAACGGCGCGGGCAAGACGACGAGCATCGGCAAGCTCGCGAAGCATCTGCAGAGCTTCGATCAATCGGTGCTGCTCGC
CGCCGGCGACACGTTCCGCGCGGCCGCGCGCGAGCAGCTCGCGATCTGGGGCGAGCGCAACAACGTGACCGTCGTCCAGC
AGGAAAGCGGCGATCCGGCCGCGGTGATTTTCGATGCGGTCAGCGCCGCGCGCGCGCGCAACATCGACGTGATGATGGCC
GACACCGCGGGCCGCCTGCCGACGCAGCTGCATCTGATGGAAGAGCTCCGGAAGGTGAAGCGCGTGATCGGCAAGGCGCA
CGACGGCGCGCCGCACGAAGTGCTGCTCGTGATCGACGCGAACACCGGCCAGAACGCGCTCGCGCAGGTGAAGGCCTTCG
ACGACGCGCTCGGCCTCACGGGCCTCATCGTCACGAAGCTCGACGGCACCGCGAAGGGCGGCATCCTCGCCGCGATCGCG
CGCCAGCGGCCGATTCCGGTGTACTTCATCGGCGTCGGCGAGAAGGTCGAGGATCTGCAGCCGTTCAGCGCGGAGGAATT
CGCGGACGCGCTGCTCGGATAA

Domains

Predicted by InterproScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(152-212)

SRP54-type protein, GTPase domain (SRP54)

(234-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q2T1C5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

50.227

100

0.51