Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   G7098_RS17765 Genome accession   NZ_CP049980
Coordinates   3757731..3759164 (+) Length   477 a.a.
NCBI ID   WP_032614481.1    Uniprot ID   A0A3E1ZUH7
Organism   Leclercia adecarboxylata strain 707804     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3752731..3764164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7098_RS17735 (G7098_17735) - 3753277..3754254 (+) 978 WP_032614470.1 calcium/sodium antiporter -
  G7098_RS17740 (G7098_17740) kdsD 3754268..3755254 (+) 987 WP_165718389.1 arabinose-5-phosphate isomerase KdsD -
  G7098_RS17745 (G7098_17745) kdsC 3755269..3755835 (+) 567 WP_032614474.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  G7098_RS17750 (G7098_17750) lptC 3755832..3756407 (+) 576 WP_165718390.1 LPS export ABC transporter periplasmic protein LptC -
  G7098_RS17755 (G7098_17755) lptA 3756376..3756951 (+) 576 WP_165718391.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  G7098_RS17760 (G7098_17760) lptB 3756958..3757683 (+) 726 WP_032614478.1 LPS export ABC transporter ATP-binding protein -
  G7098_RS17765 (G7098_17765) rpoN/rpoN1 3757731..3759164 (+) 1434 WP_032614481.1 RNA polymerase factor sigma-54 Machinery gene
  G7098_RS17770 (G7098_17770) hpf 3759187..3759474 (+) 288 WP_032614483.1 ribosome hibernation promoting factor -
  G7098_RS17775 (G7098_17775) ptsN 3759557..3760048 (+) 492 WP_032614485.1 PTS IIA-like nitrogen regulatory protein PtsN -
  G7098_RS17780 (G7098_17780) rapZ 3760094..3760948 (+) 855 WP_032614487.1 RNase adapter RapZ -
  G7098_RS17785 (G7098_17785) npr 3760945..3761217 (+) 273 WP_032614489.1 PTS phosphocarrier protein NPr -
  G7098_RS17790 (G7098_17790) mtgA 3761214..3761933 (-) 720 WP_032614632.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  G7098_RS17795 (G7098_17795) elbB 3761936..3762589 (-) 654 WP_032614491.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54186.01 Da        Isoelectric Point: 4.3155

>NTDB_id=429163 G7098_RS17765 WP_032614481.1 3757731..3759164(+) (rpoN/rpoN1) [Leclercia adecarboxylata strain 707804]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVETQQTQDNETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLDD
ILESIGDDEIDLEEVEAVLKRVQRFDPIGVAARDLRDCLLIQLSQFVKETPWLEEARLIVSEHLDLLANHDFRSLMRVTR
LKEEILKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGLWTVELNSDSIPRLQINQQYASMCTSSRNDSDNQFIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=429163 G7098_RS17765 WP_032614481.1 3757731..3759164(+) (rpoN/rpoN1) [Leclercia adecarboxylata strain 707804]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTGGCAATGACGCCGCAGTTACAACAGGCCATTCGTCTGTTGCA
GCTGTCGACGCTAGAACTTCAGCAGGAGCTTCAGCAAGCGCTGGACAGTAATCCCCTGCTTGAGCAAACCGATCTTCACG
ACGAGGTAGAAACCCAGCAAACGCAGGATAACGAAACGCTCGACAGCGTCGACGCGCTGGAGCAAAAAGAGATGCCTGAC
GAGCTTCCTCTTGATGCCAGCTGGGATGAGATCTACACCGCCGGTACGCCATCCGGTACGCGCGCCGATTACCAGGATGA
TGAGTTGCCGGTGTATCAGGGCGAAACCACCCAGTCCCTCCAGGATTACCTGATGTGGCAGGTGGAGCTAACACCGTTTT
CCGATACCGATCGCGCCATTGCCACCTCCATTGTCGATGCCGTCGATGAGACCGGCTATTTAACCGTGCCGCTGGACGAC
ATTCTGGAAAGCATCGGTGATGATGAGATCGATCTTGAAGAGGTCGAAGCGGTCCTGAAGCGCGTGCAGCGTTTTGATCC
GATTGGCGTTGCGGCACGTGATTTACGTGACTGCCTGCTGATTCAGCTTTCGCAGTTCGTCAAAGAGACGCCGTGGCTGG
AAGAGGCCAGACTGATCGTCAGCGAGCATCTGGATCTGCTGGCCAACCACGACTTCCGCTCGTTGATGCGCGTGACGCGA
TTGAAAGAAGAGATCCTGAAAGAGGCGGTCAATCTTATTCAGTCGTTGGATCCGCGTCCGGGGCAATCCATCCAGACCAG
CGAGCCTGAGTACGTTATTCCCGATGTACTGGTACGCAAGCATAACGGCCTGTGGACCGTGGAGCTGAATTCCGACAGCA
TCCCGCGCCTGCAAATTAATCAGCAATACGCCTCGATGTGCACCAGTTCGCGCAACGACTCGGATAACCAGTTTATCCGC
AGCAACCTGCAGGAGGCGCGCTGGCTGATCAAAAGCCTCGAGAGCCGCAATGATACGCTGCTACGCGTGAGCCGCTGCAT
CGTAGAGCAACAGCAGGCCTTCTTTGAACAAGGTGAAGAATTTATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACGATTTCCCGCGTGACGACGCAGAAGTATCTGCACAGCCCGCGCGGTATTTTTGAGCTTAAG
TATTTTTTCTCCAGCCATGTCAATACCGAAGGCGGTGGCGAAGCTTCGTCTACAGCCATCCGGGCGCTGGTGAAAAAATT
GATCGCCGCGGAAAACCCCGCGAAACCACTGAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAAGGTATCATGGTGG
CACGCCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAACTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3E1ZUH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48