Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   G4G26_RS12840 Genome accession   NZ_CP049924
Coordinates   2515469..2515879 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain So1b     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2510469..2520879
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G4G26_RS12810 (G4G26_12845) - 2510555..2510695 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  G4G26_RS12815 (G4G26_12850) yqeF 2511001..2511732 (-) 732 WP_015714294.1 SGNH/GDSL hydrolase family protein -
  G4G26_RS12820 (G4G26_12855) cwlH 2511985..2512737 (-) 753 WP_015714296.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  G4G26_RS12825 (G4G26_12860) yqeD 2512924..2513550 (+) 627 WP_015714297.1 TVP38/TMEM64 family protein -
  G4G26_RS12830 (G4G26_12865) gnd 2513569..2514462 (-) 894 WP_015714298.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  G4G26_RS12835 (G4G26_12870) yqeB 2514714..2515436 (+) 723 WP_010886572.1 hypothetical protein -
  G4G26_RS12840 (G4G26_12875) nucA/comI 2515469..2515879 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  G4G26_RS12845 (G4G26_12880) - 2516075..2516422 (+) 348 Protein_2495 sigma-70 family RNA polymerase sigma factor -
  G4G26_RS12850 (G4G26_12885) spoIVCA 2516453..2517913 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  G4G26_RS20950 - 2517871..2518049 (-) 179 Protein_2497 hypothetical protein -
  G4G26_RS12855 (G4G26_12890) arsC 2518404..2518823 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  G4G26_RS12860 (G4G26_12895) acr3 2518835..2519875 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  G4G26_RS12865 (G4G26_12900) yqcK 2519898..2520338 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  G4G26_RS12870 (G4G26_12905) arsR 2520399..2520716 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=428746 G4G26_RS12840 WP_009967785.1 2515469..2515879(-) (nucA/comI) [Bacillus subtilis strain So1b]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=428746 G4G26_RS12840 WP_009967785.1 2515469..2515879(-) (nucA/comI) [Bacillus subtilis strain So1b]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529