Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   G8A07_RS01705 Genome accession   NZ_CP049919
Coordinates   363997..365358 (-) Length   453 a.a.
NCBI ID   WP_195795415.1    Uniprot ID   A0A7S9C4B4
Organism   Roseateles sp. DAIF2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 358997..370358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8A07_RS01685 (G8A07_01685) - 359916..360794 (+) 879 WP_195795411.1 LysR family transcriptional regulator -
  G8A07_RS01690 (G8A07_01690) - 360798..361157 (-) 360 WP_195795412.1 antibiotic biosynthesis monooxygenase -
  G8A07_RS01695 (G8A07_01695) - 361246..362052 (+) 807 WP_195795413.1 ABC transporter substrate-binding protein -
  G8A07_RS01700 (G8A07_01700) - 362073..364010 (+) 1938 WP_195795414.1 diguanylate cyclase domain-containing protein -
  G8A07_RS01705 (G8A07_01705) radA 363997..365358 (-) 1362 WP_195795415.1 DNA repair protein RadA Machinery gene
  G8A07_RS01710 (G8A07_01710) - 365370..366176 (-) 807 WP_195795416.1 hypothetical protein -
  G8A07_RS01715 (G8A07_01715) - 366313..366939 (+) 627 WP_195795417.1 LysE family transporter -
  G8A07_RS01720 (G8A07_01720) - 367021..367920 (+) 900 WP_195795418.1 DMT family transporter -
  G8A07_RS01725 (G8A07_01725) arfB 367889..368293 (-) 405 WP_195795419.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  G8A07_RS01730 (G8A07_01730) - 368302..368796 (-) 495 WP_195795420.1 acyl-CoA thioesterase -
  G8A07_RS01735 (G8A07_01735) alr 368813..369922 (-) 1110 WP_195795421.1 alanine racemase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 47466.84 Da        Isoelectric Point: 7.3645

>NTDB_id=428677 G8A07_RS01705 WP_195795415.1 363997..365358(-) (radA) [Roseateles sp. DAIF2]
MAKEKSIYTCSDCGATSPKWLGKCPGCGAWNTLIEGVAEPAGGARNRYQALAKSQPVATLSEIEATDFERTPTGQDELDR
VLGGGIVAGGVVLIGGDPGIGKSTLLLQAIEDLSARVPVLYVTGEESGAQIAMRSRRLGLSGAKVRVLAEINLEKILATL
EAERPAFCVIDSIQTIYSEQLSSAPGSVAQVRECAAHLTRTAKASGCAMVLVGHVTKEGALAGPRVLEHIVDTVLYFEGD
THSSFRLVRAIKNRFGAVNEIGVFAMTDKGLKGVANPSAIFLSTHGEPVPGSCVLVTLEGTRPMLVELQALVDSGGPSAR
RLSVGLDRDRLAMLLAVLHRHAGVATMDQDVFVNAVGGVRIGEPAADLAVLLAIQGSLRGRALPKGFIAFGEVGLAGEVR
PAPRGQERLKEAAKLGFSVAVVPKANAPKKPIEGLTVHAVERIEEAMDLVRGL

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=428677 G8A07_RS01705 WP_195795415.1 363997..365358(-) (radA) [Roseateles sp. DAIF2]
ATGGCGAAAGAAAAATCCATCTACACCTGCTCCGACTGCGGTGCGACCAGCCCCAAGTGGCTGGGCAAATGCCCGGGCTG
CGGCGCCTGGAACACGCTGATCGAGGGCGTCGCCGAGCCCGCGGGCGGCGCCAGGAATCGCTATCAGGCGCTGGCCAAGA
GCCAGCCGGTGGCGACCCTGTCCGAGATCGAGGCCACCGATTTCGAGCGCACGCCCACCGGCCAGGATGAACTGGACCGG
GTGCTGGGCGGCGGCATCGTGGCCGGCGGCGTGGTGCTGATCGGCGGCGATCCGGGCATCGGCAAGTCGACCCTGTTGCT
GCAGGCGATCGAGGACCTGTCGGCGCGCGTGCCGGTGCTTTATGTCACCGGCGAGGAATCGGGCGCGCAGATCGCGATGC
GCTCGCGCCGCCTGGGCCTGTCGGGCGCCAAGGTGCGGGTGCTGGCCGAGATCAACCTGGAGAAGATCCTCGCGACCCTG
GAGGCCGAGCGGCCGGCCTTCTGCGTGATCGACTCGATCCAGACCATCTATTCCGAGCAGCTCAGCTCGGCACCCGGCTC
GGTGGCCCAGGTGCGCGAATGCGCGGCGCACCTGACGCGCACCGCCAAGGCCAGCGGCTGCGCGATGGTGCTGGTCGGCC
ATGTCACGAAAGAAGGCGCGCTGGCCGGCCCGCGCGTGCTGGAGCACATCGTCGACACGGTGCTGTACTTCGAGGGCGAC
ACGCATTCCAGCTTCCGCCTGGTGCGCGCGATCAAGAACCGCTTCGGCGCGGTCAACGAGATCGGCGTGTTCGCAATGAC
CGACAAGGGCCTGAAGGGCGTGGCCAACCCGAGCGCGATCTTCCTCTCGACCCATGGCGAGCCGGTGCCGGGCAGCTGCG
TGCTGGTCACGCTGGAGGGCACCCGGCCGATGCTGGTCGAGCTGCAAGCCCTGGTGGACAGCGGCGGCCCGAGCGCGCGC
CGCCTCTCGGTGGGCCTGGACCGCGACCGGCTGGCGATGCTGCTGGCGGTGCTGCACCGCCATGCCGGCGTCGCGACGAT
GGACCAGGACGTGTTCGTCAACGCGGTCGGCGGCGTGCGCATCGGCGAGCCGGCGGCCGACCTGGCGGTGCTGCTGGCGA
TCCAGGGCTCGCTGCGCGGCCGCGCCCTGCCCAAGGGCTTCATCGCCTTCGGCGAGGTCGGCCTGGCCGGCGAGGTGCGG
CCCGCGCCGCGCGGCCAGGAGCGCCTGAAGGAGGCCGCCAAGCTGGGCTTCAGCGTCGCGGTGGTGCCGAAGGCGAATGC
GCCGAAGAAGCCGATCGAGGGCCTGACCGTGCATGCGGTCGAGCGCATCGAGGAGGCGATGGACCTGGTCAGGGGCCTGT
GA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(61-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7S9C4B4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.14

100

0.486
  radA Streptococcus mitis SK321

47.033

100

0.472
  radA Streptococcus mitis NCTC 12261

46.667

99.338

0.464
  radA Streptococcus pneumoniae TIGR4

48.478

94.26

0.457
  radA Streptococcus pneumoniae D39

48.478

94.26

0.457
  radA Streptococcus pneumoniae Rx1

48.478

94.26

0.457
  radA Streptococcus pneumoniae R6

48.478

94.26

0.457