Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   G8D99_RS02490 Genome accession   NZ_CP049916
Coordinates   525434..526213 (+) Length   259 a.a.
NCBI ID   WP_166327468.1    Uniprot ID   A0A6G8S872
Organism   Acinetobacter lanii strain 185     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 520434..531213
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8D99_RS02465 (G8D99_02465) - 521737..522150 (+) 414 WP_166322271.1 hypothetical protein -
  G8D99_RS02470 (G8D99_02470) - 522260..523090 (+) 831 WP_166322273.1 XAC2610-related protein -
  G8D99_RS02475 (G8D99_02475) iscX 523262..523462 (+) 201 WP_166322275.1 Fe-S cluster assembly protein IscX -
  G8D99_RS02480 (G8D99_02480) ndk 523571..524002 (+) 432 WP_035265623.1 nucleoside-diphosphate kinase -
  G8D99_RS02485 (G8D99_02485) rlmN 524126..525361 (+) 1236 WP_166322277.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  G8D99_RS02490 (G8D99_02490) pilF 525434..526213 (+) 780 WP_166327468.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  G8D99_RS02495 (G8D99_02495) - 526204..526998 (+) 795 WP_166322279.1 RodZ domain-containing protein -
  G8D99_RS02500 (G8D99_02500) ispG 527022..528140 (+) 1119 WP_166322281.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  G8D99_RS02505 (G8D99_02505) hisS 528151..529446 (+) 1296 WP_166322283.1 histidine--tRNA ligase -
  G8D99_RS02510 (G8D99_02510) - 529473..530174 (+) 702 WP_166322285.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 259 a.a.        Molecular weight: 29207.32 Da        Isoelectric Point: 9.5569

>NTDB_id=428633 G8D99_RS02490 WP_166327468.1 525434..526213(+) (pilF) [Acinetobacter lanii strain 185]
MTVCLSALVVQGCQTTKSGSVVKKDPEKAAKVRTQMAAEYIRSGDLDAAKRALDQALEADSRDSTANMMMGVLLQQEGSK
LNMEKADSYFSRAISLDPKNAQARNNYGTYLYQLKRYNDAIQQFSIAGATLGYDQRYRALENLGRTYIKLGDMANAEKAF
KQALQVNRQSTVSMLELAEILYLQHNAVAARQLYEQYVRLVGQKEQGARALWIGLRIARANGDTMGMQVLVNQMRALFPE
SQEYQRYLQLQYNTEAVWK

Nucleotide


Download         Length: 780 bp        

>NTDB_id=428633 G8D99_RS02490 WP_166327468.1 525434..526213(+) (pilF) [Acinetobacter lanii strain 185]
ATGACGGTGTGTTTGTCTGCATTGGTTGTACAAGGGTGTCAGACGACAAAATCGGGAAGTGTAGTTAAAAAAGACCCTGA
AAAAGCAGCCAAAGTACGTACCCAAATGGCGGCTGAATATATTCGATCGGGTGATCTAGACGCGGCAAAGCGCGCGCTGG
ATCAGGCGCTTGAGGCAGATTCTCGAGATTCAACCGCCAATATGATGATGGGTGTGTTACTGCAACAAGAGGGCAGTAAA
CTCAATATGGAAAAGGCCGATAGTTATTTTAGTCGTGCCATTTCCCTAGACCCTAAAAATGCACAGGCGCGTAATAATTA
CGGCACATATTTATACCAGTTAAAGCGTTATAATGATGCCATCCAGCAATTTAGTATTGCGGGTGCAACATTGGGTTATG
ATCAGCGTTATAGAGCCTTGGAAAATTTAGGTCGAACCTATATCAAACTTGGCGATATGGCGAATGCTGAAAAAGCATTC
AAACAAGCATTGCAAGTTAACCGTCAATCGACGGTTTCAATGTTAGAGTTAGCTGAGATTTTATATTTGCAGCACAATGC
TGTTGCAGCACGCCAACTCTACGAACAATATGTCCGTTTGGTTGGTCAAAAGGAGCAAGGTGCTCGTGCACTTTGGATTG
GCTTACGTATCGCGCGTGCCAATGGTGATACGATGGGAATGCAAGTGTTGGTCAATCAAATGCGCGCATTATTTCCAGAG
AGTCAAGAATATCAACGTTATTTGCAATTACAGTACAATACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(90-122)

Tetratricopeptide repeat (TPR_8)

(138-169)

Tetratricopeptide repeat (TPR_16)

(37-73)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8S872

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

75.954

100

0.768
  pilF Acinetobacter baylyi ADP1

70.196

98.456

0.691