Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   G7Y90_RS05710 Genome accession   NZ_CP049902
Coordinates   1137592..1138713 (-) Length   373 a.a.
NCBI ID   WP_250163636.1    Uniprot ID   -
Organism   Psychrobacter sp. WY6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1132592..1143713
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7Y90_RS05680 - 1132919..1133254 (+) 336 WP_109592356.1 c-type cytochrome -
  G7Y90_RS05685 - 1133593..1134261 (+) 669 WP_045443487.1 c-type cytochrome -
  G7Y90_RS05690 - 1134708..1135718 (+) 1011 WP_250163634.1 hypothetical protein -
  G7Y90_RS05695 - 1135767..1136216 (+) 450 WP_250163635.1 RnfH family protein -
  G7Y90_RS05700 bamE 1136261..1136680 (-) 420 WP_045455336.1 outer membrane protein assembly factor BamE -
  G7Y90_RS05705 fur 1137008..1137442 (+) 435 WP_055123484.1 ferric iron uptake transcriptional regulator -
  G7Y90_RS05710 pilU 1137592..1138713 (-) 1122 WP_250163636.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G7Y90_RS05715 - 1138882..1139936 (-) 1055 Protein_1120 type IV pilus twitching motility protein PilT -
  G7Y90_RS05720 - 1140407..1141053 (+) 647 Protein_1121 YggS family pyridoxal phosphate-dependent enzyme -
  G7Y90_RS05725 - 1141065..1141799 (-) 735 WP_250163637.1 hemolysin family protein -
  G7Y90_RS05730 - 1141738..1142184 (-) 447 WP_250163638.1 CNNM domain-containing protein -
  G7Y90_RS05735 - 1142195..1142335 (-) 141 WP_250163639.1 CNNM domain-containing protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41730.92 Da        Isoelectric Point: 6.7814

>NTDB_id=428457 G7Y90_RS05710 WP_250163636.1 1137592..1138713(-) (pilU) [Psychrobacter sp. WY6]
MDIDKLLQLMINKNGSDLFITADVAPSMKVNGKILPVGKTPLTAEQTMRLVKGVMTPNQQKEFEETNECQFAIADEAQQA
RFRVSAFIQRDMAGMILRKIENKIPTVEELRLPPALKELAMKKRGIILLVGATGTGKSTTLASMIGHRNQNSHGHIITIE
DPIEFVHKHRGCIITQREVGIDTHSFEAGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFETSRHNQLFMDLSLNLQAIIAQQLIPTPDGKGRRAAIEILLNSQLISDYIRKGEVHEIKDVMTRSRDSGMQTFDQ
ALFDLYEQGEITYKEAILHADSPNDLRLKIKLSSKNGTANLDEGTDNLSLDIS

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=428457 G7Y90_RS05710 WP_250163636.1 1137592..1138713(-) (pilU) [Psychrobacter sp. WY6]
ATGGACATTGATAAACTATTGCAGTTAATGATCAATAAAAATGGCTCGGACCTTTTTATTACCGCTGATGTAGCGCCTTC
CATGAAAGTGAATGGCAAAATTCTGCCTGTCGGTAAAACGCCTTTAACTGCTGAGCAAACCATGCGATTGGTCAAAGGCG
TCATGACACCCAACCAGCAAAAAGAGTTTGAAGAGACCAACGAGTGCCAATTTGCCATTGCTGATGAAGCACAGCAAGCA
CGTTTTCGGGTCAGTGCCTTTATACAGCGTGATATGGCAGGGATGATTTTACGGAAAATTGAGAATAAAATTCCGACTGT
CGAAGAGCTACGTTTGCCGCCCGCGTTAAAAGAGTTGGCAATGAAAAAACGCGGTATCATTCTGCTTGTGGGTGCAACAG
GTACAGGTAAATCAACGACCCTTGCTTCTATGATCGGACACCGTAATCAAAACTCGCATGGTCACATCATTACCATCGAA
GATCCTATCGAGTTTGTCCACAAACATCGTGGTTGTATTATTACCCAGCGTGAAGTTGGTATCGATACCCATTCATTTGA
GGCAGGGCTAAAAAACACGTTACGCCAAGCACCTGATGTTATTTTGATTGGTGAAATTCGTAACCGTGAAGTCATGAGTT
ACGCGATTCAGTACGCTGAGACAGGGCATTTAGTGTTTGCAACCTTACACGCCAACAACGCCAACCAAGCGATTGACCGT
ATCATTCACTTTTTTGAAACCAGTCGCCACAATCAGTTATTTATGGATTTGTCGCTCAACTTGCAAGCCATTATTGCGCA
GCAGCTCATTCCCACTCCTGATGGTAAAGGACGCCGTGCGGCTATCGAAATTTTGTTAAATTCACAGCTGATTAGTGACT
ATATCCGTAAAGGCGAGGTTCATGAAATCAAAGATGTCATGACACGATCTCGGGATAGCGGCATGCAAACCTTTGATCAG
GCTCTTTTTGACTTATATGAGCAAGGAGAAATCACCTATAAAGAAGCCATTCTTCATGCTGACTCTCCTAATGATTTGCG
CCTAAAAATCAAACTCAGCAGTAAAAATGGCACAGCCAATCTGGATGAAGGTACGGATAACTTATCATTAGATATCAGTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(39-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.488

100

0.665
  pilU Acinetobacter baylyi ADP1

67.042

95.174

0.638
  pilU Vibrio cholerae strain A1552

51.075

99.732

0.509
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.818

97.051

0.416
  pilT Acinetobacter nosocomialis M2

40.233

91.957

0.37
  pilT Acinetobacter baumannii D1279779

40.233

91.957

0.37
  pilT Acinetobacter baumannii strain A118

40.233

91.957

0.37
  pilT Legionella pneumophila strain ERS1305867

41.071

90.08

0.37
  pilT Legionella pneumophila strain Lp02

41.071

90.08

0.37
  pilT Vibrio cholerae strain A1552

40.896

89.812

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.896

89.812

0.367
  pilT Pseudomonas aeruginosa PAK

39.306

92.761

0.365