Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   G7081_RS03055 Genome accession   NZ_CP049886
Coordinates   591783..593153 (+) Length   456 a.a.
NCBI ID   WP_166007278.1    Uniprot ID   A0A6G8AM32
Organism   Vagococcus coleopterorum strain HDW17A     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 586783..598153
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7081_RS03030 (G7081_03030) - 587650..587955 (+) 306 WP_166007273.1 rhodanese-like domain-containing protein -
  G7081_RS03035 (G7081_03035) - 587957..589606 (+) 1650 WP_166007274.1 FAD-dependent oxidoreductase -
  G7081_RS03040 (G7081_03040) - 589620..589877 (+) 258 WP_166007275.1 metal-sensing transcriptional repressor -
  G7081_RS03045 (G7081_03045) - 589963..591090 (+) 1128 WP_166007276.1 VanZ family protein -
  G7081_RS03050 (G7081_03050) - 591113..591643 (+) 531 WP_166007277.1 dUTP diphosphatase -
  G7081_RS03055 (G7081_03055) radA 591783..593153 (+) 1371 WP_166007278.1 DNA repair protein RadA Machinery gene
  G7081_RS03060 (G7081_03060) - 593179..594282 (+) 1104 WP_166007279.1 PIN/TRAM domain-containing protein -
  G7081_RS03065 (G7081_03065) ispF 594295..594777 (+) 483 WP_166007280.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  G7081_RS03070 (G7081_03070) gltX 594782..596245 (+) 1464 WP_166007281.1 glutamate--tRNA ligase -
  G7081_RS03075 (G7081_03075) cysS 596517..597929 (+) 1413 WP_166007282.1 cysteine--tRNA ligase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50068.81 Da        Isoelectric Point: 8.0276

>NTDB_id=428353 G7081_RS03055 WP_166007278.1 591783..593153(+) (radA) [Vagococcus coleopterorum strain HDW17A]
MAKKAKTQFECQACGYISPKYLGRCPNCGQWNEMMEVKLQDDTNRNSRVSLAGKRTTATKLGDVTSKKEPRVKTKIEELN
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSQQLHQSGGKVLYVSGEESAEQIKMRAERLSIEGNDFYVYPETDMSLIRQ
TIEEMAPDYVIIDSIQTMMQPEISSATGSVSQVRETTAELMQIAKTNQIAIFIVGHVTKEGALAGPRMLEHMVDTVLYFE
GDRHHTFRILRAVKNRFGSTNEIGIFEMKERGLVEVANPSEAFLEERLDGATGSAIVVSMEGTRPILVEIQSLVTPSMFG
NAKRTATGLDYNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLDEPAIDLAIAVAIASSYKEKGTKPTECFIGEIGLTG
EIRRVNRIEQRIMEAKKLGFKKMYIPKNNLQGWEPPKGIEVVGVSTLGEALHKIFG

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=428353 G7081_RS03055 WP_166007278.1 591783..593153(+) (radA) [Vagococcus coleopterorum strain HDW17A]
ATGGCTAAAAAAGCAAAAACACAATTTGAATGTCAGGCCTGTGGTTATATTTCTCCGAAATACTTAGGTCGTTGTCCGAA
CTGTGGACAATGGAATGAAATGATGGAAGTGAAATTGCAAGATGATACAAATCGAAATAGTCGAGTTAGTTTAGCTGGGA
AAAGGACGACAGCAACTAAATTAGGTGATGTGACATCAAAGAAAGAACCGCGTGTTAAGACTAAGATAGAAGAATTAAAC
CGTGTGCTTGGTGGCGGAGTTGTCCCAGGCTCATTAGTTCTAATCGGTGGGGATCCAGGGATTGGGAAATCTACATTATT
ATTACAAGTTTCGCAACAACTTCATCAAAGCGGTGGGAAAGTATTATATGTATCTGGAGAGGAAAGTGCGGAACAAATAA
AAATGCGAGCAGAACGCTTGAGCATTGAAGGTAATGATTTTTATGTTTACCCAGAAACAGATATGTCATTAATTCGTCAA
ACAATTGAAGAGATGGCGCCAGATTATGTCATCATTGATTCCATTCAAACAATGATGCAGCCAGAAATTTCTAGTGCAAC
GGGTAGCGTTAGTCAAGTTCGGGAAACAACTGCCGAGTTGATGCAAATTGCTAAAACAAACCAAATTGCTATTTTTATTG
TGGGACATGTAACAAAAGAAGGAGCTTTAGCTGGTCCAAGAATGTTGGAACACATGGTTGATACGGTTCTTTATTTTGAG
GGTGATCGACACCATACTTTTAGAATTTTACGAGCTGTTAAAAATCGTTTTGGCTCAACCAACGAGATTGGGATTTTTGA
AATGAAAGAACGCGGATTGGTTGAAGTGGCTAATCCCTCTGAGGCATTTTTAGAAGAACGACTTGATGGTGCTACAGGTT
CAGCTATTGTTGTATCAATGGAGGGAACACGACCAATTTTAGTAGAAATTCAATCGTTGGTGACACCGAGTATGTTTGGG
AATGCTAAACGAACAGCGACGGGGCTAGATTACAATCGAGTATCACTAATTATGGCAGTTCTAGAAAAAAGAGCAGGTTT
ATTGTTGCAAAATCAAGATGCGTATTTAAAGGCAGCGGGTGGCGTTAAGTTAGATGAACCAGCAATTGATTTGGCAATCG
CTGTTGCCATAGCTTCAAGTTATAAAGAAAAAGGGACGAAACCAACTGAGTGCTTTATCGGTGAGATTGGATTAACTGGA
GAAATTCGCCGTGTTAACCGTATAGAGCAACGCATTATGGAAGCTAAAAAACTAGGGTTTAAGAAAATGTACATTCCAAA
AAATAATTTACAAGGTTGGGAGCCACCTAAAGGGATTGAAGTAGTGGGCGTATCTACCTTAGGCGAAGCTTTACATAAAA
TTTTCGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-34)

AAA domain (AAA_25)

(71-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8AM32

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.561

100

0.746
  radA Streptococcus mitis SK321

74.342

100

0.743
  radA Streptococcus pneumoniae Rx1

74.342

100

0.743
  radA Streptococcus pneumoniae D39

74.342

100

0.743
  radA Streptococcus pneumoniae R6

74.342

100

0.743
  radA Streptococcus pneumoniae TIGR4

74.342

100

0.743
  radA Bacillus subtilis subsp. subtilis str. 168

66.228

100

0.662