Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   G9H43_RS01530 Genome accession   NZ_CP049830
Coordinates   317386..318825 (+) Length   479 a.a.
NCBI ID   WP_005314410.1    Uniprot ID   A4SHX7
Organism   Aeromonas salmonicida subsp. masoucida strain RZ6S-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 312386..323825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G9H43_RS01500 (G9H43_01495) mlaF 312963..313763 (-) 801 WP_011898278.1 phospholipid ABC transporter ATP-binding protein MlaF -
  G9H43_RS01505 (G9H43_01500) - 313971..314966 (+) 996 WP_011898279.1 KpsF/GutQ family sugar-phosphate isomerase -
  G9H43_RS01510 (G9H43_01505) kdsC 314966..315520 (+) 555 WP_042468044.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  G9H43_RS01515 (G9H43_01510) lptC 315517..316077 (+) 561 WP_042468042.1 LPS export ABC transporter periplasmic protein LptC -
  G9H43_RS01520 (G9H43_01515) lptA 316064..316591 (+) 528 WP_005314414.1 lipopolysaccharide transport periplasmic protein LptA -
  G9H43_RS01525 (G9H43_01520) lptB 316595..317320 (+) 726 WP_005314412.1 LPS export ABC transporter ATP-binding protein -
  G9H43_RS01530 (G9H43_01525) rpoN/rpoN1 317386..318825 (+) 1440 WP_005314410.1 RNA polymerase factor sigma-54 Machinery gene
  G9H43_RS01535 (G9H43_01530) hpf 318847..319134 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  G9H43_RS01540 (G9H43_01535) ptsN 319137..319583 (+) 447 WP_005314406.1 PTS IIA-like nitrogen regulatory protein PtsN -
  G9H43_RS01545 (G9H43_01540) rapZ 319626..320492 (+) 867 WP_005314405.1 RNase adapter RapZ -
  G9H43_RS01550 (G9H43_01545) npr 320513..320785 (+) 273 WP_005314403.1 PTS phosphocarrier protein NPr -
  G9H43_RS01555 (G9H43_01550) - 320804..322708 (-) 1905 WP_042468040.1 transcription antiterminator -
  G9H43_RS01560 (G9H43_01555) - 322812..323552 (-) 741 WP_005314402.1 KDGP aldolase family protein -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53962.92 Da        Isoelectric Point: 4.3718

>NTDB_id=428061 G9H43_RS01530 WP_005314410.1 317386..318825(+) (rpoN/rpoN1) [Aeromonas salmonicida subsp. masoucida strain RZ6S-1]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDVQETSPEEPVDDSQLDSSDAMAQDQMP
DELPVDTTWEDVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDDSGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYAPELPWLNEAREVIKEHMDLLGNRDYRTLARK
TKLKESDLKEVLDLIQQLEPRPGNGIIQSDSQYVIPDVTVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVATCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGRECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=428061 G9H43_RS01530 WP_005314410.1 317386..318825(+) (rpoN/rpoN1) [Aeromonas salmonicida subsp. masoucida strain RZ6S-1]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAACAATCCTTTGCTTGAGCAAGAGGAGAATGACG
TTCAGGAGACCAGCCCGGAAGAGCCCGTCGACGACAGTCAGCTCGACTCCAGCGATGCCATGGCCCAGGATCAGATGCCG
GACGAACTGCCGGTAGATACCACCTGGGAAGATGTCTATTCAGCCGGCACAGCCCAGAGCGCAGGCCCCATCCACGATGG
GGAGGACAGCGTCTATCAAGGGGAAACCACCGAGAACCTGCAGGACTATCTGCTGTGGCAGATGCGCCTCACCCCGTTCA
GCGATCTGGATGCCGCCATTGCGCTGGCCATCATAGATGCCATCGATGACTCCGGTTATCTGACCGTCTCTCTCGAAGAT
ATACAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGACGAGGTCGAGGCAGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCGCGCTCGGTGCAGGAGTGTCTGCTGATCCAGCTTGGCCAGTACGCTCCTGAACTGCCCT
GGCTCAATGAGGCCAGGGAGGTAATCAAGGAGCACATGGACTTGCTCGGCAACCGTGATTACCGCACCCTGGCCCGCAAG
ACCAAGCTCAAGGAGAGTGACCTCAAGGAGGTGCTGGACCTTATCCAGCAACTGGAGCCCAGGCCGGGTAACGGCATCAT
ACAGAGTGACTCCCAGTACGTGATCCCGGATGTCACCGTCGTCAAGAAGGGCAACAAATGGGTCGTTGAGCTCAACCCTG
ATTCTGCACCGCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGTCAGGCGCGTACCAGCCAGGATACCCAGTTC
ATTCGCTCCCACTTGCAAGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCGAC
CTGTATTGTCGAGCAACAACAGGGCTTTTTCGAGTATGGTGAAGAAGCCATGAAGCCCATGGTGCTCAACGATATCGCCG
AGGCGGTGGAGATGCATGAGTCGACCATTTCCAGGGTAACGACCCAGAAATACATGCATACCCCCCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGTCATGTGAACACAGAAGGAGGAAGAGAGTGCTCATCGACCGCAATTCGCGCGCTGATCAA
GAAGCTGGTGACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A4SHX7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.58

100

0.484