Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   G8E09_RS16420 Genome accession   NZ_CP049806
Coordinates   3469053..3469853 (-) Length   266 a.a.
NCBI ID   WP_199977444.1    Uniprot ID   -
Organism   Acinetobacter pittii strain A1254     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3464053..3474853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8E09_RS16400 (G8E09_16390) - 3465118..3465816 (-) 699 WP_002114884.1 YfgM family protein -
  G8E09_RS16405 (G8E09_16395) hisS 3465845..3467137 (-) 1293 WP_017385736.1 histidine--tRNA ligase -
  G8E09_RS16410 (G8E09_16400) ispG 3467134..3468249 (-) 1116 WP_002115078.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  G8E09_RS16415 (G8E09_16405) - 3468271..3469062 (-) 792 WP_002114935.1 helix-turn-helix domain-containing protein -
  G8E09_RS16420 (G8E09_16410) pilF 3469053..3469853 (-) 801 WP_199977444.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  G8E09_RS16425 (G8E09_16415) rlmN 3469868..3471103 (-) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  G8E09_RS16430 (G8E09_16420) ndk 3471227..3471658 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  G8E09_RS16435 (G8E09_16425) iscX 3471772..3471972 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  G8E09_RS16440 (G8E09_16430) - 3472072..3472623 (-) 552 WP_005066176.1 phosphatase PAP2 family protein -
  G8E09_RS16445 (G8E09_16435) - 3472648..3473937 (-) 1290 WP_075383343.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30086.32 Da        Isoelectric Point: 9.0417

>NTDB_id=427994 G8E09_RS16420 WP_199977444.1 3469053..3469853(-) (pilF) [Acinetobacter pittii strain A1254]
MSIPKLKTVLCMGIAVAFLASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=427994 G8E09_RS16420 WP_199977444.1 3469053..3469853(-) (pilF) [Acinetobacter pittii strain A1254]
TTGAGTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCAGTTGCATTTCTGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCAGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCTGAACATTATTTTAAACGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCTTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

94.361

100

0.944
  pilF Acinetobacter baylyi ADP1

76.154

97.744

0.744