Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilW   Type   Machinery gene
Locus tag   G8E00_RS14320 Genome accession   NZ_CP049801
Coordinates   3157261..3158184 (-) Length   307 a.a.
NCBI ID   WP_166225687.1    Uniprot ID   A0A6G8RYT3
Organism   Acinetobacter shaoyimingii strain 323-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3152261..3163184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8E00_RS14310 (G8E00_14305) - 3152516..3156454 (-) 3939 WP_166225681.1 PilC/PilY family type IV pilus protein -
  G8E00_RS14315 (G8E00_14310) - 3156470..3157264 (-) 795 WP_166225684.1 PilX N-terminal domain-containing pilus assembly protein -
  G8E00_RS14320 (G8E00_14315) pilW 3157261..3158184 (-) 924 WP_166225687.1 PilW family protein Machinery gene
  G8E00_RS14325 (G8E00_14320) pilV 3158186..3158698 (-) 513 WP_320077573.1 type IV pilus modification protein PilV Machinery gene
  G8E00_RS14330 (G8E00_14325) - 3158698..3159186 (-) 489 WP_166225693.1 Tfp pilus assembly protein FimT/FimU -
  G8E00_RS14335 (G8E00_14330) ispH 3159325..3160278 (-) 954 WP_166225696.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  G8E00_RS14340 (G8E00_14335) gmk 3160412..3161032 (+) 621 WP_166011179.1 guanylate kinase -
  G8E00_RS14345 (G8E00_14340) rpoZ 3161108..3161389 (+) 282 WP_166011181.1 DNA-directed RNA polymerase subunit omega -

Sequence

Protein


Download         Length: 307 a.a.        Molecular weight: 33245.50 Da        Isoelectric Point: 4.6076

>NTDB_id=427917 G8E00_RS14320 WP_166225687.1 3157261..3158184(-) (pilW) [Acinetobacter shaoyimingii strain 323-1]
MKIKIINIKNTQGFTLVELLVALGLGIIISAAALQLFMGGLISTRMQQASAELQDSGLFGLEYIARDVRLVNFGNITRPN
LNDETTSGGIVLSRSTTDGNFNVISSAVGDDDITKSDLPSNVNIKSDQLTIQFIAPNDMVNCEGQLVEKDQYVIQRYFLR
PDANGQANDLALACDANKPNGTTAASIENFGGDDQGEIIMPRVDHLKFYLGAQSNKGENLGYYTIPKYIEVANNARSASN
EPPRIVSVKIEVIVRSKDSTASKMVDPSVGTEATDDSNLSSTDSKTPYLRRLYVTTVALRNALGETL

Nucleotide


Download         Length: 924 bp        

>NTDB_id=427917 G8E00_RS14320 WP_166225687.1 3157261..3158184(-) (pilW) [Acinetobacter shaoyimingii strain 323-1]
ATGAAAATTAAAATAATAAATATTAAAAATACCCAAGGTTTTACATTGGTTGAGTTACTGGTTGCGTTGGGACTAGGAAT
TATTATTAGTGCTGCAGCTTTGCAATTGTTTATGGGAGGATTGATTTCTACTCGTATGCAACAAGCGAGTGCTGAATTAC
AAGATAGTGGTTTATTTGGTCTTGAATATATTGCCAGAGATGTTCGATTGGTTAATTTTGGAAATATTACCCGCCCCAAT
TTAAATGATGAAACAACTTCAGGTGGTATTGTTTTATCAAGATCAACAACTGATGGCAATTTCAATGTAATCAGTTCTGC
CGTAGGTGATGATGATATTACTAAGTCCGATTTGCCGAGTAACGTAAACATCAAAAGCGATCAACTAACCATTCAATTTA
TTGCACCTAATGATATGGTGAATTGTGAAGGTCAACTTGTAGAAAAAGATCAGTATGTTATTCAAAGATATTTTTTGAGA
CCAGATGCCAATGGGCAAGCTAATGATCTTGCATTGGCATGTGATGCAAATAAACCTAATGGAACAACGGCTGCTTCTAT
TGAGAATTTCGGTGGTGATGATCAGGGTGAGATCATTATGCCACGTGTCGACCATTTAAAATTTTATTTAGGTGCACAAT
CAAATAAAGGCGAAAATTTAGGTTATTACACAATTCCTAAATATATTGAAGTGGCAAATAATGCCCGATCTGCTTCCAAT
GAGCCTCCAAGAATTGTCTCCGTGAAAATTGAAGTGATTGTTCGATCCAAAGATAGTACAGCAAGTAAAATGGTCGACCC
TTCAGTAGGAACTGAAGCAACAGATGATTCAAATCTGAGTTCAACTGATTCAAAGACACCATATTTGCGTCGATTATATG
TGACGACAGTAGCCTTACGTAATGCTTTGGGGGAAACACTATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(9-34)

Type IV Pilus-assembly protein W (PilW)

(156-301)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8RYT3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilW Acinetobacter baumannii D1279779

47.239

100

0.502