Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   G8E00_RS13495 Genome accession   NZ_CP049801
Coordinates   2991486..2992292 (-) Length   268 a.a.
NCBI ID   WP_166225402.1    Uniprot ID   A0A6G8RYD5
Organism   Acinetobacter shaoyimingii strain 323-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2986486..2997292
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8E00_RS13475 (G8E00_13470) - 2987520..2988221 (-) 702 WP_166225395.1 tetratricopeptide repeat protein -
  G8E00_RS13480 (G8E00_13475) hisS 2988253..2989548 (-) 1296 WP_166225397.1 histidine--tRNA ligase -
  G8E00_RS13485 (G8E00_13480) ispG 2989545..2990660 (-) 1116 WP_166012136.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  G8E00_RS13490 (G8E00_13485) - 2990683..2991495 (-) 813 WP_166225399.1 helix-turn-helix domain-containing protein -
  G8E00_RS13495 (G8E00_13490) pilF 2991486..2992292 (-) 807 WP_166225402.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  G8E00_RS13500 (G8E00_13495) rlmN 2992328..2993560 (-) 1233 WP_166225404.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  G8E00_RS13505 (G8E00_13500) ndk 2993698..2994129 (-) 432 WP_166012140.1 nucleoside-diphosphate kinase -
  G8E00_RS13510 (G8E00_13505) iscX 2994239..2994439 (-) 201 WP_166012141.1 Fe-S cluster assembly protein IscX -
  G8E00_RS13515 (G8E00_13510) - 2994549..2995292 (-) 744 WP_166225407.1 hypothetical protein -
  G8E00_RS13520 (G8E00_13515) - 2995392..2995943 (-) 552 WP_166012143.1 phosphatase PAP2 family protein -
  G8E00_RS13525 (G8E00_13520) - 2996129..2996965 (-) 837 WP_166225409.1 XAC2610-related protein -

Sequence

Protein


Download         Length: 268 a.a.        Molecular weight: 30063.33 Da        Isoelectric Point: 9.4323

>NTDB_id=427914 G8E00_RS13495 WP_166225402.1 2991486..2992292(-) (pilF) [Acinetobacter shaoyimingii strain 323-1]
MYKKTIVCAAMCLSALVVQGCQTTGSSAAFVKKDPEKAVKVRTQMAAEYIRTGNLDAAKRVLDQALETDSKDSSANMMMG
VLLQQEGSDANLQKADGYFTRAIASDPKNAQARNNYGTYLYQMQRYNEAVQQLNVAGSTLGYEQRARALENLGLTYLKLG
QVANAEKTFKQALQVNRDSTISMLELSEILYEQQNVVVARQLYEQYVRIVGQKNQGARALWVGIRVARANQDQLGMQVLV
NQLRALFPDSLEYQRYLKLQYTTEAVWK

Nucleotide


Download         Length: 807 bp        

>NTDB_id=427914 G8E00_RS13495 WP_166225402.1 2991486..2992292(-) (pilF) [Acinetobacter shaoyimingii strain 323-1]
ATGTATAAAAAAACAATCGTATGCGCTGCGATGTGTTTGTCTGCATTGGTTGTACAAGGATGTCAGACAACGGGTAGTTC
AGCAGCATTTGTAAAAAAAGATCCTGAAAAAGCGGTGAAAGTCCGTACACAAATGGCAGCTGAATATATACGCACTGGCA
ACTTAGATGCGGCAAAACGAGTGCTTGATCAAGCGCTTGAAACAGACTCTAAAGATTCAAGTGCCAATATGATGATGGGC
GTACTTTTGCAACAAGAAGGCAGCGATGCTAATTTGCAAAAAGCCGATGGTTATTTTACCCGTGCAATCGCATCAGATCC
GAAAAATGCGCAGGCGAGAAACAATTACGGCACGTATTTGTATCAAATGCAGCGATATAATGAAGCAGTTCAACAGTTAA
ATGTGGCTGGTTCTACCCTCGGATATGAACAACGCGCACGCGCGCTGGAAAATTTGGGACTGACCTATTTAAAATTGGGG
CAAGTTGCGAATGCTGAAAAAACGTTCAAACAAGCGTTGCAAGTGAATCGTGATTCTACCATTTCAATGTTAGAGTTATC
TGAGATTTTATATGAGCAACAAAACGTCGTTGTAGCACGTCAACTCTATGAACAATATGTCCGTATAGTTGGTCAGAAAA
ATCAAGGTGCAAGAGCACTTTGGGTGGGTATCCGTGTTGCTCGTGCCAATCAAGATCAGTTGGGAATGCAAGTTTTAGTC
AATCAACTTCGTGCATTGTTCCCAGATAGTCTAGAATATCAACGTTATTTGAAATTACAGTACACTACTGAGGCCGTATG
GAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_1)

(146-178)

Tetratricopeptide repeat (TPR_8)

(42-71)

Tetratricopeptide repeat (TPR_17)

(99-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8RYD5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

70.943

98.881

0.701
  pilF Acinetobacter baylyi ADP1

69.811

98.881

0.69