Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   G3T20_RS23145 Genome accession   NZ_CP049736
Coordinates   4914612..4916048 (-) Length   478 a.a.
NCBI ID   WP_080666485.1    Uniprot ID   -
Organism   Bordetella hinzii strain FY01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4909612..4921048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3T20_RS23120 (G3T20_23145) - 4910728..4911411 (+) 684 WP_029577791.1 hypothetical protein -
  G3T20_RS23125 (G3T20_23150) bfr 4911474..4911950 (-) 477 WP_029577792.1 bacterioferritin -
  G3T20_RS23130 (G3T20_23155) - 4912264..4913175 (-) 912 WP_165555271.1 DMT family transporter -
  G3T20_RS23135 (G3T20_23160) - 4913283..4914152 (+) 870 WP_048940413.1 LysR family transcriptional regulator -
  G3T20_RS23140 (G3T20_23165) - 4914213..4914491 (-) 279 WP_029577795.1 (2Fe-2S)-binding protein -
  G3T20_RS23145 (G3T20_23170) rpoN/rpoN1 4914612..4916048 (-) 1437 WP_080666485.1 RNA polymerase factor sigma-54 Machinery gene
  G3T20_RS23150 (G3T20_23175) - 4916047..4916736 (+) 690 WP_029577797.1 hypothetical protein -
  G3T20_RS23155 (G3T20_23180) leuA 4917112..4918821 (+) 1710 WP_029577798.1 2-isopropylmalate synthase -
  G3T20_RS23160 (G3T20_23185) - 4918910..4920199 (-) 1290 WP_029577799.1 amidase -

Sequence

Protein


Download         Length: 478 a.a.        Molecular weight: 52715.12 Da        Isoelectric Point: 4.9223

>NTDB_id=427407 G3T20_RS23145 WP_080666485.1 4914612..4916048(-) (rpoN/rpoN1) [Bordetella hinzii strain FY01]
MQNIVPMTRPALELRPGQHLTLTPQLQQSIRLLQLSSLELEAEISQALAENPLLERDESPADQVDSDPQREASVDHEDAA
PDTELRFDEMPGSGGVYPDDDIDTPARDDTLREHLLLQLSLTRAAPRDAALATLLIDELDDNGYLGSPLDEILQWLPPEL
EVEEDELRAALSLLQSFDPPGIGARDMAECLLLQLRQPDIGRLPEAAQPAVLACARQICMQHLALLATGNPARLREILRV
DEATLRAARALIVRLEPRPGRAWTAPAADYAVPDVLVRKTRQGWQAVLNSAVMPRLQINGLYAQMLSNQREAAYAGLNAQ
LQQARWMIRNVEQRFDTILRVAQAIVAHQAEFFSRGPAAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTLELKRF
FGTGVATDSGDSASATAVQAHIRQMVAEENRARPLSDSQIMERLASQGIVIARRTVAKYREALRIAPAAVRKAQAAAR

Nucleotide


Download         Length: 1437 bp        

>NTDB_id=427407 G3T20_RS23145 WP_080666485.1 4914612..4916048(-) (rpoN/rpoN1) [Bordetella hinzii strain FY01]
ATGCAAAATATTGTACCTATGACTCGTCCCGCCTTAGAACTTCGGCCCGGTCAGCATTTGACCCTGACCCCGCAGCTGCA
ACAGTCCATCCGGCTGCTGCAGCTCTCTTCGCTGGAGCTGGAGGCGGAAATCTCCCAGGCCCTGGCTGAAAATCCGTTGT
TGGAACGCGACGAAAGTCCGGCCGACCAGGTCGACAGCGACCCCCAGCGCGAAGCCAGCGTCGACCATGAGGACGCCGCC
CCGGACACCGAATTGCGCTTTGACGAGATGCCGGGCTCGGGCGGCGTCTATCCGGACGACGACATCGATACACCGGCGCG
CGATGACACGTTGCGCGAGCACCTGCTGCTGCAGCTGAGCCTGACGCGCGCCGCCCCGCGCGATGCCGCCCTGGCCACGC
TGCTCATCGACGAGCTGGATGACAACGGCTACCTGGGCTCGCCGCTCGACGAAATCCTGCAATGGCTGCCGCCCGAACTG
GAGGTCGAGGAGGACGAGCTGCGCGCGGCCTTGAGCCTGCTGCAATCCTTCGATCCGCCGGGCATCGGCGCGCGCGACAT
GGCCGAGTGCCTGCTCCTGCAGTTGCGCCAGCCTGATATCGGGCGCCTGCCCGAGGCCGCCCAGCCCGCGGTACTGGCCT
GTGCGCGGCAGATCTGCATGCAGCACCTGGCGCTGCTGGCCACCGGCAATCCGGCGCGGCTGCGCGAGATATTGCGGGTC
GACGAGGCCACGCTGCGCGCGGCGCGCGCGCTCATCGTGCGGCTGGAACCCCGCCCCGGCCGCGCCTGGACCGCGCCGGC
CGCGGACTATGCCGTGCCCGATGTGTTGGTGCGCAAGACCCGGCAGGGCTGGCAGGCCGTGCTCAACAGCGCGGTGATGC
CGCGCCTGCAGATCAACGGCCTGTATGCCCAGATGCTGTCCAACCAGCGCGAAGCGGCCTACGCAGGCCTGAATGCCCAG
CTGCAGCAGGCACGCTGGATGATACGCAACGTCGAGCAGCGTTTTGACACCATCCTGCGGGTGGCCCAGGCCATCGTCGC
GCACCAGGCGGAGTTCTTCAGCCGCGGGCCCGCCGCCATGCGGCCGCTGATCCTCAAGGACATCGCGGGCGAGCTCGGCC
TGCACGAATCCACGATTTCGCGCGCCACGACGCAGAAGTATATGCTCACTCCGTTCGGCACGCTGGAGCTCAAGCGGTTT
TTCGGCACGGGCGTGGCGACCGATAGCGGCGACTCCGCCTCGGCCACGGCGGTGCAGGCCCATATCCGGCAGATGGTGGC
CGAGGAAAATCGGGCCAGGCCCCTGTCCGATAGCCAGATCATGGAGCGCCTGGCCAGCCAAGGCATCGTGATCGCCCGCC
GCACCGTGGCCAAGTACCGCGAGGCCCTGCGTATCGCCCCGGCCGCCGTGCGCAAGGCCCAGGCCGCGGCGCGCTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-472)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(106-302)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.198

100

0.531