Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   DER53_RS16650 Genome accession   NZ_CP049703
Coordinates   3230265..3231311 (-) Length   348 a.a.
NCBI ID   WP_015864484.1    Uniprot ID   A0A6G9J647
Organism   Parageobacillus toebii NBRC 107807 strain DSM 14590     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3225265..3236311
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DER53_RS16610 (DER53_16605) - 3226054..3226845 (+) 792 WP_015864476.1 YqhG family protein -
  DER53_RS16615 (DER53_16610) - 3226906..3227085 (-) 180 WP_015864477.1 YqzE family protein -
  DER53_RS16620 (DER53_16615) comGG 3227280..3227666 (-) 387 WP_015864478.1 competence type IV pilus minor pilin ComGG -
  DER53_RS16625 (DER53_16620) comGF 3227683..3228105 (-) 423 WP_015864479.1 competence type IV pilus minor pilin ComGF -
  DER53_RS16630 (DER53_16625) comGE 3228102..3228425 (-) 324 WP_062678736.1 competence type IV pilus minor pilin ComGE -
  DER53_RS16635 (DER53_16630) comGD 3228409..3228852 (-) 444 WP_015864481.1 competence type IV pilus minor pilin ComGD -
  DER53_RS16640 (DER53_16635) comGC 3228836..3229132 (-) 297 WP_015864482.1 competence type IV pilus major pilin ComGC Machinery gene
  DER53_RS16645 (DER53_16640) comGB 3229147..3230172 (-) 1026 WP_062752900.1 competence type IV pilus assembly protein ComGB -
  DER53_RS16650 (DER53_16645) comGA 3230265..3231311 (-) 1047 WP_015864484.1 competence type IV pilus ATPase ComGA Machinery gene
  DER53_RS16655 (DER53_16650) - 3231512..3232210 (+) 699 WP_062752902.1 helix-turn-helix domain-containing protein -
  DER53_RS16660 (DER53_16655) - 3232395..3232637 (+) 243 WP_008879854.1 DUF2626 family protein -
  DER53_RS16665 (DER53_16660) - 3232686..3233829 (-) 1144 Protein_3220 SAM-dependent methyltransferase -
  DER53_RS16670 (DER53_16665) - 3234110..3234742 (-) 633 WP_015864487.1 MBL fold metallo-hydrolase -
  DER53_RS16675 (DER53_16670) - 3235102..3235278 (+) 177 WP_015864488.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 39600.17 Da        Isoelectric Point: 9.0889

>NTDB_id=427328 DER53_RS16650 WP_015864484.1 3230265..3231311(-) (comGA) [Parageobacillus toebii NBRC 107807 strain DSM 14590]
MNEIEYVADRLIKEASLLHVSDIHIVPRKDDAIVRFRLDGLLMEKEALTKEMCERLITHFKFLAGMDIGERRRPQSGAME
ARHQEEIIHLRLSTLPTSYDESLVIRLLPQNFFIPRSQLSLFANATKTLLSLFRQPQGLIIFTGPTGSGKTSTLYTLLRI
CQYEWHRNVITLEDPVEKRIDNILQVQINEKAGITYTTGLKAVLRHDPDVIMIGEIRDAETAKIAVRSAMTGHLIATTMH
TKNAVGAIYRLREFGIPLGDIEQTLLAVVAQRLVDLVCPFCGEHCSIFCRKYRPIRRAAVHELLYGNALSNAIQSVQTKE
KTHHYYTLQHVIRKGVALGFLPAHLLYR

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=427328 DER53_RS16650 WP_015864484.1 3230265..3231311(-) (comGA) [Parageobacillus toebii NBRC 107807 strain DSM 14590]
ATGAATGAAATTGAATATGTTGCCGATCGTCTCATAAAAGAAGCGAGTTTGCTTCATGTATCTGACATTCATATCGTTCC
GCGCAAAGACGATGCGATTGTGCGTTTCCGGTTAGATGGATTGCTGATGGAAAAGGAAGCGCTGACAAAAGAAATGTGCG
AGCGGCTTATTACGCATTTTAAATTTTTAGCAGGGATGGACATTGGCGAACGCCGCCGTCCGCAAAGCGGAGCGATGGAA
GCAAGGCATCAGGAAGAAATCATTCACTTACGCCTCTCCACATTACCGACATCGTATGATGAAAGCCTCGTTATCCGGCT
TCTTCCGCAGAATTTTTTTATTCCTCGATCACAACTTTCTCTATTTGCAAATGCCACGAAAACGTTACTTTCCCTTTTTC
GGCAGCCCCAAGGATTAATTATTTTTACAGGACCAACTGGATCAGGCAAAACGTCAACATTATATACGTTATTGCGCATT
TGTCAATATGAGTGGCATCGCAATGTCATCACATTGGAAGACCCTGTTGAAAAGCGAATCGACAACATATTGCAAGTGCA
AATTAATGAGAAAGCGGGAATTACGTATACAACCGGTTTAAAAGCTGTTTTGCGCCATGATCCGGATGTGATTATGATCG
GCGAAATTCGCGACGCCGAGACCGCAAAAATTGCGGTACGCTCAGCAATGACGGGACATTTGATTGCTACGACCATGCAT
ACAAAAAACGCTGTTGGTGCGATTTACCGTTTGCGTGAATTCGGGATTCCGCTTGGAGATATTGAGCAAACATTGCTCGC
CGTTGTCGCACAGCGGCTCGTGGACTTAGTATGCCCGTTTTGCGGTGAACATTGCTCCATATTTTGCCGTAAATATCGCC
CCATTCGCCGCGCTGCTGTCCATGAACTGCTGTATGGGAATGCTTTGTCGAACGCCATTCAATCCGTACAAACAAAGGAA
AAGACGCATCACTACTATACGTTGCAACACGTTATTCGAAAAGGAGTTGCTCTTGGATTTTTGCCAGCACACCTTCTTTA
CAGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G9J647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.726

98.563

0.569
  pilB Glaesserella parasuis strain SC1401

42.157

87.931

0.371