Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   C2753_RS02850 Genome accession   NZ_CP049680
Coordinates   544127..545491 (+) Length   454 a.a.
NCBI ID   WP_215278978.1    Uniprot ID   -
Organism   Polynucleobacter paneuropaeus strain AP-RePozz9-5-G2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 539127..550491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2753_RS02830 (C2753_02830) - 540840..541079 (-) 240 WP_133256482.1 hypothetical protein -
  C2753_RS02840 (C2753_02840) - 541648..543588 (+) 1941 WP_215278384.1 potassium transporter Kup -
  C2753_RS02845 (C2753_02845) - 543585..544088 (+) 504 WP_215275342.1 hypothetical protein -
  C2753_RS02850 (C2753_02850) radA 544127..545491 (+) 1365 WP_215278978.1 DNA repair protein RadA Machinery gene
  C2753_RS02855 (C2753_02855) prmB 545488..546384 (-) 897 WP_112209045.1 50S ribosomal protein L3 N(5)-glutamine methyltransferase -
  C2753_RS02860 (C2753_02860) dapE 546414..547565 (-) 1152 WP_215278979.1 succinyl-diaminopimelate desuccinylase -
  C2753_RS02865 (C2753_02865) dapD 547572..548399 (-) 828 WP_112237815.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48390.22 Da        Isoelectric Point: 7.6369

>NTDB_id=426503 C2753_RS02850 WP_215278978.1 544127..545491(+) (radA) [Polynucleobacter paneuropaeus strain AP-RePozz9-5-G2]
MAKAKTIYVCQSCGGTSAKWQGQCPSCQAWNTMEEGLPESSTPSRFQGLAQSLPRQKLAAITAEDLPRFSTGVEEFDRVL
GGGLVPGGVVLLGGDPGIGKSTLLLQALAEMSSAGMHVLYSSGEESAAQIALRAKRIALEAPALEVLAEIQLEKLLSIME
TVKPQVLVVDSIQTLYSEAFSSAPGSVAQVRECAAQLTRAAKSSGICVLMVGHVTKDGHLAGPRVLEHIVDTVLYFEGDT
HSSFRLVRSIKNRFGAVNELGVFAMTEKGLRGVSNPSAIFLSQHEQMVPGACVLVTQEGTRPLLVEIQALVDTAHIPNPR
RLAVGLEQHRLAMLLAVLHRHAGVACFDQDVFLNAVGGVKITEPAADLAVLFAIQSSIRNRALPKELIVFGEVGLAGEIR
PCPRGQERLKEAAKLGFKLAIIPKANMPKNKIPGLKVIPVERIDQAIAAANELS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=426503 C2753_RS02850 WP_215278978.1 544127..545491(+) (radA) [Polynucleobacter paneuropaeus strain AP-RePozz9-5-G2]
TTGGCTAAAGCTAAAACGATTTATGTCTGTCAATCATGTGGCGGAACCTCTGCTAAATGGCAAGGGCAGTGTCCTTCCTG
TCAGGCTTGGAACACAATGGAGGAAGGACTTCCTGAGTCGAGTACACCATCCCGCTTTCAGGGTTTGGCTCAATCTTTGC
CAAGACAAAAACTTGCAGCAATTACTGCGGAAGATTTACCTCGTTTTAGTACTGGGGTAGAAGAGTTCGATCGCGTTTTA
GGTGGTGGCTTAGTTCCGGGTGGTGTTGTACTTTTGGGTGGTGATCCCGGCATTGGTAAATCCACCTTGTTACTTCAGGC
TTTGGCTGAGATGAGTTCAGCTGGTATGCATGTTCTCTACAGTAGTGGCGAAGAGTCAGCTGCGCAAATTGCATTACGGG
CTAAACGGATTGCTTTAGAAGCCCCTGCGCTTGAAGTATTGGCTGAGATTCAGCTAGAGAAGCTGTTGTCCATCATGGAA
ACCGTGAAGCCTCAAGTGCTCGTGGTGGACTCTATTCAAACTTTGTATTCAGAAGCCTTCAGTTCTGCGCCAGGCTCAGT
AGCCCAGGTGCGTGAGTGTGCCGCACAACTCACGAGGGCCGCCAAGTCCAGTGGTATCTGTGTATTGATGGTTGGGCACG
TCACCAAAGACGGTCATCTTGCTGGTCCTCGTGTCTTAGAACATATTGTGGACACGGTTTTGTATTTTGAGGGCGATACG
CATTCTTCATTTAGATTGGTGCGTTCGATTAAAAACCGCTTTGGCGCTGTCAATGAGCTCGGTGTTTTTGCGATGACTGA
AAAAGGTCTGCGTGGTGTATCGAATCCATCAGCGATTTTCTTATCGCAGCACGAACAGATGGTGCCAGGAGCTTGTGTTC
TGGTAACGCAAGAAGGTACCAGACCACTCTTAGTGGAGATCCAGGCTTTAGTTGATACCGCCCATATCCCCAACCCAAGA
AGATTGGCGGTAGGGTTGGAGCAGCATCGCTTGGCAATGCTGCTGGCGGTCTTACATCGTCATGCTGGGGTCGCTTGCTT
TGATCAAGACGTATTCTTAAACGCAGTAGGCGGCGTCAAAATCACTGAGCCTGCTGCTGACTTAGCTGTTCTATTCGCTA
TTCAATCCTCAATTCGAAATCGTGCACTACCCAAAGAATTGATCGTCTTCGGTGAAGTCGGTTTAGCTGGGGAGATTCGC
CCATGCCCGAGAGGTCAGGAACGATTGAAGGAAGCTGCCAAATTGGGCTTTAAGCTTGCAATCATTCCGAAGGCCAATAT
GCCAAAGAATAAAATTCCGGGACTTAAAGTAATCCCTGTTGAAAGAATTGACCAGGCGATTGCTGCAGCGAATGAGCTGA
GCTAG

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(360-450)

AAA domain (AAA_25)

(79-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.558

99.559

0.493
  radA Streptococcus pneumoniae Rx1

46.222

99.119

0.458
  radA Streptococcus pneumoniae D39

46.222

99.119

0.458
  radA Streptococcus pneumoniae R6

46.222

99.119

0.458
  radA Streptococcus pneumoniae TIGR4

46.222

99.119

0.458
  radA Streptococcus mitis SK321

47.14

96.256

0.454
  radA Streptococcus mitis NCTC 12261

47.14

96.256

0.454