Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   G7L40_RS21260 Genome accession   NZ_CP049598
Coordinates   4630518..4630964 (+) Length   148 a.a.
NCBI ID   WP_019687466.1    Uniprot ID   A0A378Y019
Organism   Paenibacillus polymyxa strain 2020     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4625518..4635964
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7L40_RS21225 (G7L40_21260) - 4625529..4625954 (-) 426 WP_019687474.1 hypothetical protein -
  G7L40_RS21230 (G7L40_21265) - 4626263..4626607 (+) 345 WP_019687473.1 hypothetical protein -
  G7L40_RS21235 (G7L40_21270) - 4626812..4627069 (-) 258 WP_014599905.1 hypothetical protein -
  G7L40_RS21240 (G7L40_21275) - 4627100..4627606 (-) 507 WP_019687471.1 hypothetical protein -
  G7L40_RS21245 (G7L40_21280) nucA/comI 4628219..4628665 (+) 447 WP_019687469.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  G7L40_RS21250 (G7L40_21285) - 4628840..4629550 (+) 711 WP_019687468.1 C39 family peptidase -
  G7L40_RS21255 (G7L40_21290) - 4629673..4630128 (+) 456 WP_019687467.1 hypothetical protein -
  G7L40_RS21260 (G7L40_21295) nucA/comI 4630518..4630964 (+) 447 WP_019687466.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  G7L40_RS21265 (G7L40_21300) - 4631057..4631299 (+) 243 WP_019687465.1 hypothetical protein -
  G7L40_RS27505 - 4631325..4632092 (-) 768 WP_019687464.1 hypothetical protein -
  G7L40_RS21275 (G7L40_21310) - 4632251..4633297 (+) 1047 WP_019687463.1 fibronectin type III domain-containing protein -
  G7L40_RS21280 (G7L40_21315) - 4633551..4634696 (+) 1146 WP_182096049.1 hypothetical protein -
  G7L40_RS21285 (G7L40_21320) - 4634711..4635097 (+) 387 WP_019687461.1 hypothetical protein -
  G7L40_RS28040 - 4635233..4635289 (+) 57 WP_241764007.1 hypothetical protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16168.45 Da        Isoelectric Point: 5.2598

>NTDB_id=426388 G7L40_RS21260 WP_019687466.1 4630518..4630964(+) (nucA/comI) [Paenibacillus polymyxa strain 2020]
MFRWIFTVMITILLAGCSVQQDVVKTTESDPVAVPVTQVSADMIKLEFPSAKYPETAQHIKEAIAAGKSPVCTIDREGAD
HNRELSLKGVPTKKGKDRDEWPMAMCAEGGEGADIKYISPKDNRGAGSWVGHKLDDYADGTKVEFIVN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=426388 G7L40_RS21260 WP_019687466.1 4630518..4630964(+) (nucA/comI) [Paenibacillus polymyxa strain 2020]
ATGTTTAGATGGATATTTACCGTAATGATTACAATCCTATTAGCTGGCTGCAGCGTACAACAAGACGTTGTAAAGACCAC
TGAGAGCGACCCTGTAGCCGTTCCAGTAACGCAGGTGTCGGCAGACATGATTAAGCTGGAATTTCCGTCAGCCAAGTACC
CAGAAACGGCCCAGCACATCAAGGAGGCTATTGCAGCCGGTAAGTCACCAGTATGCACCATAGACCGCGAAGGAGCCGAC
CATAACCGTGAACTATCTCTGAAAGGTGTACCCACTAAAAAAGGCAAAGACCGCGACGAGTGGCCTATGGCGATGTGTGC
AGAAGGTGGAGAAGGTGCAGACATAAAGTACATAAGTCCAAAGGATAATCGTGGAGCTGGATCGTGGGTAGGTCACAAGT
TAGACGACTACGCAGATGGGACAAAAGTTGAATTTATCGTGAATTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378Y019

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.874

69.595

0.493