Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   G6543_RS02585 Genome accession   NZ_CP049363
Coordinates   548315..549115 (+) Length   266 a.a.
NCBI ID   WP_002027454.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain ATCC 17978     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 543315..554115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G6543_RS02560 - 544225..545523 (+) 1299 WP_000348500.1 glycosyltransferase family 4 protein -
  G6543_RS02565 - 545548..546099 (+) 552 WP_000669567.1 phosphatase PAP2 family protein -
  G6543_RS02570 iscX 546199..546399 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  G6543_RS02575 ndk 546513..546944 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  G6543_RS02580 rlmN 547067..548299 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  G6543_RS02585 pilF 548315..549115 (+) 801 WP_002027454.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  G6543_RS02590 - 549106..549897 (+) 792 WP_000405164.1 helix-turn-helix domain-containing protein -
  G6543_RS02595 ispG 549919..551034 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  G6543_RS02600 hisS 551031..552323 (+) 1293 WP_000095264.1 histidine--tRNA ligase -
  G6543_RS02605 - 552352..553050 (+) 699 WP_000058788.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30124.47 Da        Isoelectric Point: 9.2307

>NTDB_id=426304 G6543_RS02585 WP_002027454.1 548315..549115(+) (pilF) [Acinetobacter baumannii strain ATCC 17978]
MSTPKLKIMLCMGVAVALLTSGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=426304 G6543_RS02585 WP_002027454.1 548315..549115(+) (pilF) [Acinetobacter baumannii strain ATCC 17978]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGACAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTACTTTAAGCGTGCAATTTCATCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCCTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726