Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   GYO_RS35160 Genome accession   NC_016047
Coordinates   2633478..2633888 (-) Length   136 a.a.
NCBI ID   WP_014114486.1    Uniprot ID   -
Organism   Bacillus spizizenii TU-B-10     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2628478..2638888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GYO_RS35120 (GYO_2839) - 2628490..2628630 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  GYO_RS35130 (GYO_2840) - 2628936..2629667 (-) 732 WP_014114481.1 SGNH/GDSL hydrolase family protein -
  GYO_RS35140 (GYO_2841) - 2629919..2630671 (-) 753 WP_014114482.1 N-acetylmuramoyl-L-alanine amidase -
  GYO_RS35145 (GYO_2842) - 2630866..2631492 (+) 627 WP_014114483.1 TVP38/TMEM64 family protein -
  GYO_RS35150 (GYO_2843) gnd 2631511..2632404 (-) 894 WP_014114484.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  GYO_RS35155 (GYO_2844) - 2632729..2633445 (+) 717 WP_014114485.1 hypothetical protein -
  GYO_RS35160 (GYO_2845) nucA/comI 2633478..2633888 (-) 411 WP_014114486.1 sporulation-specific Dnase NucB Machinery gene
  GYO_RS35165 (GYO_2846) sigK 2634084..2634812 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  GYO_RS44330 - 2634888..2634969 (+) 82 Protein_2623 hypothetical protein -
  GYO_RS43430 - 2634935..2635131 (-) 197 Protein_2624 recombinase family protein -
  GYO_RS44120 - 2635089..2635267 (-) 179 Protein_2625 hypothetical protein -
  GYO_RS35180 (GYO_2848) - 2635834..2637084 (-) 1251 WP_014114487.1 DHHA1 domain-containing protein -
  GYO_RS35185 (GYO_2849) - 2637156..2638262 (-) 1107 WP_014114488.1 D-TA family PLP-dependent enzyme -
  GYO_RS35190 (GYO_2850) - 2638259..2638591 (-) 333 WP_238528878.1 RidA family protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14938.98 Da        Isoelectric Point: 5.1643

>NTDB_id=42601 GYO_RS35160 WP_014114486.1 2633478..2633888(-) (nucA/comI) [Bacillus spizizenii TU-B-10]
MKKWMAGLFLAAAVLLCLMVPQQIQGASLYDKVLYFPLSRYPETGDHIKDAIADGHSDICTIDRDGADKRRQESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSGYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=42601 GYO_RS35160 WP_014114486.1 2633478..2633888(-) (nucA/comI) [Bacillus spizizenii TU-B-10]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
TTCTTTGTATGACAAAGTGTTGTATTTTCCGCTGTCTCGTTACCCGGAAACGGGGGATCATATTAAAGATGCGATTGCAG
ATGGTCATTCTGATATTTGCACCATCGACAGGGATGGAGCGGACAAAAGGCGGCAGGAATCATTAAAAGGAATTCCGACG
AAGCCGGGCTATGATCGGGATGAGTGGCCGATGGCGGTTTGTGAGGAAGGCGGCGCAGGAGCTGATGTCCGATATGTCAC
GCCTTCTGATAATCGCGGGGCCGGCTCGTGGGTAGGGAATCAAATGAGCGGTTATCCTGACGGCACCAGAGTGCTCTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment