Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   G5C61_RS10025 Genome accession   NZ_CP049296
Coordinates   1844094..1845146 (-) Length   350 a.a.
NCBI ID   WP_029320205.1    Uniprot ID   -
Organism   Priestia megaterium strain S188     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1839094..1850146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5C61_RS09985 - 1840110..1840934 (+) 825 WP_013059169.1 YqhG family protein -
  G5C61_RS09990 - 1840958..1841143 (-) 186 WP_013059170.1 YqzE family protein -
  G5C61_RS09995 comGG 1841187..1841561 (-) 375 WP_043976241.1 competence type IV pilus minor pilin ComGG -
  G5C61_RS10000 comGF 1841551..1841988 (-) 438 WP_013059172.1 competence type IV pilus minor pilin ComGF -
  G5C61_RS10005 - 1841985..1842323 (-) 339 WP_028410645.1 type II secretion system protein -
  G5C61_RS10010 comGD 1842307..1842789 (-) 483 WP_223260330.1 competence type IV pilus minor pilin ComGD -
  G5C61_RS10015 comGC 1842731..1843042 (-) 312 WP_164796496.1 competence type IV pilus major pilin ComGC -
  G5C61_RS10020 comGB 1843057..1844103 (-) 1047 WP_028410643.1 competence type IV pilus assembly protein ComGB -
  G5C61_RS10025 comGA 1844094..1845146 (-) 1053 WP_029320205.1 competence type IV pilus ATPase ComGA Machinery gene
  G5C61_RS10030 - 1845416..1845799 (-) 384 WP_013059178.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  G5C61_RS10035 - 1846061..1846297 (+) 237 WP_013059179.1 DUF2626 domain-containing protein -
  G5C61_RS10040 - 1846335..1846967 (-) 633 WP_034648929.1 MBL fold metallo-hydrolase -
  G5C61_RS10045 - 1847176..1847352 (+) 177 WP_013059181.1 DUF2759 domain-containing protein -
  G5C61_RS10050 - 1847388..1848290 (-) 903 WP_075419614.1 M14 family metallocarboxypeptidase -
  G5C61_RS10055 - 1848402..1849370 (-) 969 WP_013059183.1 ROK family glucokinase -
  G5C61_RS10060 - 1849373..1849579 (-) 207 WP_013059184.1 YqgQ family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39651.06 Da        Isoelectric Point: 9.0433

>NTDB_id=425935 G5C61_RS10025 WP_029320205.1 1844094..1845146(-) (comGA) [Priestia megaterium strain S188]
MTEIEQFATYIIRDACECRSSDVHIVPRKKDAVIQLRIDQELILKRVISKEMSERLISHFKFLGGMDIGERRKPQNGALT
TSIDHYQVNLRLSTLPTSHDESLVIRLLPHQQSTSLKHLSLFPEHTKKLISLLKFSHGLIIFTGPTGSGKTTTLYTLLHE
VKHLFNRNVITLEDPIEKPSETVLQVQVNERAGITYTTGLKAILRHDPDIIMVGEIRDSETAKIAVRAALTGHLVLTTMH
TKDTKGSLYRLLEFGVSFQEMEQTLVAVAAQRLVEIRCPLCSGKCHPACKKMRKYRQSSIYELLYGKELSAVMREVKGEN
ADYDYKTLRDVILKGIALGYLYPHSLDGWG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=425935 G5C61_RS10025 WP_029320205.1 1844094..1845146(-) (comGA) [Priestia megaterium strain S188]
TTGACCGAAATTGAACAGTTTGCAACTTACATTATACGAGATGCATGTGAGTGCCGAAGTTCTGATGTGCATATTGTGCC
CCGAAAGAAAGATGCTGTGATTCAGCTGCGAATTGACCAAGAGCTTATTTTAAAGAGGGTTATTTCAAAGGAAATGTCTG
AACGGCTCATTTCGCATTTTAAATTTTTAGGGGGAATGGATATTGGCGAGCGCAGAAAACCTCAAAATGGTGCATTGACC
ACTTCCATTGATCACTATCAAGTAAATTTGCGTTTATCAACTCTTCCTACTTCTCATGATGAAAGTCTTGTTATTCGGCT
TTTGCCTCATCAACAATCTACATCTCTCAAACATTTATCTCTTTTTCCTGAACATACTAAAAAGCTTATTTCTCTCTTGA
AATTCTCTCATGGATTAATTATTTTTACCGGTCCTACAGGATCAGGTAAAACCACCACTCTATATACTCTTTTACACGAA
GTAAAGCACCTTTTTAATCGAAATGTTATTACACTAGAAGATCCTATTGAAAAACCGAGTGAAACCGTGCTTCAAGTACA
GGTAAATGAACGGGCGGGGATTACGTACACGACTGGTTTAAAAGCTATATTGCGCCATGACCCAGACATTATCATGGTGG
GAGAAATAAGAGATAGTGAGACTGCCAAAATTGCTGTGAGAGCAGCGCTTACCGGACACTTGGTTTTGACAACGATGCAT
ACTAAAGATACAAAAGGATCTTTGTACAGGCTGCTTGAATTTGGCGTTAGCTTTCAAGAAATGGAACAGACTCTTGTAGC
AGTAGCTGCACAGCGCCTTGTAGAAATTCGCTGTCCGCTGTGTTCCGGAAAATGCCACCCCGCTTGTAAAAAAATGAGGA
AGTATCGTCAGTCCAGCATATACGAATTGCTTTATGGCAAGGAGCTTAGCGCAGTGATGAGAGAAGTGAAAGGAGAAAAT
GCGGATTATGATTATAAGACGCTCCGAGATGTTATTCTAAAAGGAATCGCGCTAGGCTATTTATATCCGCATTCGCTAGA
CGGATGGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.571

100

0.586