Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MFABIIPG_RS15230 Genome accession   NZ_CP049240
Coordinates   3231420..3232220 (-) Length   266 a.a.
NCBI ID   WP_215824855.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain Ax270     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3226420..3237220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MFABIIPG_RS15210 (MFABIIPG_03030) - 3227485..3228183 (-) 699 WP_000058788.1 YfgM family protein -
  MFABIIPG_RS15215 (MFABIIPG_03031) hisS 3228212..3229504 (-) 1293 WP_000095254.1 histidine--tRNA ligase -
  MFABIIPG_RS15220 (MFABIIPG_03032) ispG 3229501..3230616 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  MFABIIPG_RS15225 (MFABIIPG_03033) - 3230638..3231429 (-) 792 WP_000405163.1 helix-turn-helix domain-containing protein -
  MFABIIPG_RS15230 (MFABIIPG_03034) pilF 3231420..3232220 (-) 801 WP_215824855.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  MFABIIPG_RS15235 (MFABIIPG_03035) rlmN 3232236..3233468 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  MFABIIPG_RS15240 (MFABIIPG_03036) ndk 3233591..3234022 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  MFABIIPG_RS15245 (MFABIIPG_03037) iscX 3234136..3234336 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  MFABIIPG_RS15250 (MFABIIPG_03038) - 3234436..3234987 (-) 552 WP_000669565.1 phosphatase PAP2 family protein -
  MFABIIPG_RS15255 (MFABIIPG_03039) - 3235012..3236310 (-) 1299 WP_001991191.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30011.35 Da        Isoelectric Point: 9.5244

>NTDB_id=425680 MFABIIPG_RS15230 WP_215824855.1 3231420..3232220(-) (pilF) [Acinetobacter baumannii strain Ax270]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPGKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKSNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGNI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=425680 MFABIIPG_RS15230 WP_215824855.1 3231420..3232220(-) (pilF) [Acinetobacter baumannii strain Ax270]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGGAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAATCCAATTTAGAAAAAGCAGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCAATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_16)

(88-134)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

98.872

100

0.989
  pilF Acinetobacter baylyi ADP1

73.462

97.744

0.718